cDNA Clone CLK3

• Plasmid: pEBG6P-1 CLK3
• Parent Plasmid: pEBG6P
• Expressed: GST-CLK3
• Genbank: NM_003992.4 GI:194097434
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGCATCACTGTAAGCGATACCGCTCCCCTGAACCAGACCCGTA
CCTGAGCTACCGATGGAAGAGGAGGAGGTCCTACAGTCGGGAACATGAAG
GGAGACTGCGATACCCGTCCCGAAGGGAGCCTCCCCCACGAAGATCTCGG
TCCAGAAGCCATGACCGCCTGCCCTACCAGAGGAGGTACCGGGAGCGCCG
TGACAGCGATACATACCGGTGTGAAGAGCGGAGCCCATCCTTTGGAGAGG
ACTACTATGGACCTTCACGTTCTCGTCATCGTCGGCGATCGCGGGAGAGG
GGGCCATACCGGACCCGCAAGCATGCCCACCACTGCCACAAACGCCGCAC
CAGGTCTTGTAGCAGCGCCTCCTCGAGAAGCCAACAGAGCAGTAAGCGCA
GCAGCCGGAGTGTGGAAGATGACAAGGAGGGTCACCTGGTGTGCCGGATC
GGCGATTGGCTCCAAGAGCGATATGAGATTGTGGGGAACCTGGGTGAAGG
CACCTTTGGCAAGGTGGTGGAGTGCTTGGACCATGCCAGAGGGAAGTCTC
AGGTTGCCCTGAAGATCATCCGCAACGTGGGCAAGTACCGGGAGGCTGCC
CGGCTAGAAATCAACGTGCTCAAAAAAATCAAGGAGAAGGACAAAGAAAA
CAAGTTCCTGTGTGTCTTGATGTCTGACTGGTTCAACTTCCACGGTCACA
TGTGCATCGCCTTTGAGCTCCTGGGCAAGAACACCTTTGAGTTCCTGAAG
GAGAATAACTTCCAGCCTTACCCCCTACCACATGTCCGGCACATGGCCTA
CCAGCTCTGCCACGCCCTTAGATTTCTGCATGAGAATCAGCTGACCCATA
CAGACTTGAAACCAGAGAACATCCTGTTTGTGAATTCTGAGTTTGAAACC
CTCTACAATGAGCACAAGAGCTGTGAGGAGAAGTCAGTGAAGAACACCAG
CATCCGAGTGGCTGACTTTGGCAGTGCCACATTTGACCATGAGCACCACA
CCACCATTGTGGCCACCCGTCACTATCGCCCGCCTGAGGTGATCCTTGAG
CTGGGCTGGGCACAGCCCTGTGACGTCTGGAGCATTGGCTGCATTCTCTT
TGAGTACTACCGGGGCTTCACACTCTTCCAGACCCACGAAAACCGAGAGC
ACCTGGTGATGATGGAGAAGATCCTAGGGCCCATCCCATCACACATGATC
CACCGTACCAGGAAGCAGAAATATTTCTACAAAGGGGGCCTAGTTTGGGA
TGAGAACAGCTCTGACGGCCGGTATGTGAAGGAGAACTGCAAACCTCTGA
AGAGTTACATGCTCCAAGACTCCCTGGAGCACGTGCAGCTGTTTGACCTG
ATGAGGAGGATGTTAGAATTTGACCCTGCCCAGCGCATCACACTGGCCGA
GGCCCTGCTGCACCCCTTCTTTGCTGGCCTGACCCCTGAGGAGCGGTCCT
TCCACACCAGCCGCAACCCAAGCAGATGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMHHCKRYRSPEPD
PYLSYRWKRRRSYSREHEGRLRYPSRREPPPRRSRSRSHDRLPYQRRYRE
RRDSDTYRCEERSPSFGEDYYGPSRSRHRRRSRERGPYRTRKHAHHCHKR
RTRSCSSASSRSQQSSKRSSRSVEDDKEGHLVCRIGDWLQERYEIVGNLG
EGTFGKVVECLDHARGKSQVALKIIRNVGKYREAARLEINVLKKIKEKDK
ENKFLCVLMSDWFNFHGHMCIAFELLGKNTFEFLKENNFQPYPLPHVRHM
AYQLCHALRFLHENQLTHTDLKPENILFVNSEFETLYNEHKSCEEKSVKN
TSIRVADFGSATFDHEHHTTIVATRHYRPPEVILELGWAQPCDVWSIGCI
LFEYYRGFTLFQTHENREHLVMMEKILGPIPSHMIHRTRKQKYFYKGGLV
WDENSSDGRYVKENCKPLKSYMLQDSLEHVQLFDLMRRMLEFDPAQRITL
AEALLHPFFAGLTPEERSFHTSRNPSR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31451
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express CLK3 with N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 86004 Daltons