cDNA Clone 14-3-3 zeta

• Synonyms: YWHAZ
• Plasmid: pIRES2-mCherry 14-3-3 zeta/pEGFP
• Parent Plasmid: pIRES2 mCherry/C-GFP
• Expressed: mCherry-14-3-3 zeta/EGFP
• Genbank: NM_145690
• Tag: GFP

Sequence of Insert

gaattctATGGATAAAAATGAGCTGGTTCAGAAGGCCAAACTGGCCGAGC
AGGCTGAGCGATATGATGACATGGCAGCCTGCATGAAGTCTGTAACTGAG
CAAGGAGCTGAATTATCCAATGAGGAGAGGAATCTTCTCTCAGTTGCTTA
TAAAAATGTTGTAGGAGCCCGTAGGTCATCTTGGAGGGTCGTCTCAAGTA
TTGAACAAAAGACGGAAGGTGCTGAGAAAAAACAGCAGATGGCTCGAGAA
TACAGAGAGAAAATTGAGACGGAGCTAAGAGATATCTGCAATGATGTACT
GTCTCTTTTGGAAAAGTTCTTGATCCCCAATGCTTCACAAGCAGAGAGCA
AAGTCTTCTATTTGAAAATGAAAGGAGATTACTACCGTTACTTGGCTGAG
GTTGCCGCTGGTGATGACAAGAAAGGGATTGTCGATCAGTCACAACAAGC
ATACCAAGAAGCTTTTGAAATCAGCAAAAAGGAAATGCAACCAACACATC
CTATCAGACTGGGTCTGGCCCTTAACTTCTCTGTGTTCTATTATGAGATT
CTGAACTCCCCAGAGAAAGCCTGCTCTCTTGCAAAGACAGCTTTTGATGA
AGCCATTGCTGAACTTGATACATTAAGTGAAGAGTCATACAAAGACAGCA
CGCTAATAATGCAATTACTGAGAGACAACTTGACATTGTGGACATCGGAT
ACCCAAGGAGACGAAGCTGAAGCAGGAGAAGGAGGGGAAAATTAAggatc
c

Amino Acid Sequence

Amino acid sequence of mCherry-14-3-3 zeta.
MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWER
VMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEA
SSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNV
NIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKGSRAQASNSMDKNE
LVQKAKLAEQAERYDDMAACMKSVTEQGAELSNEERNLLSVAYKNVVGAR
RSSWRVVSSIEQKTEGAEKKQQMAREYREKIETELRDICNDVLSLLEKFL
IPNASQAESKVFYLKMKGDYYRYLAEVAAGDDKKGIVDQSQQAYQEAFEI
SKKEMQPTHPIRLGLALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDT
LSEESYKDSTLIMQLLRDNLTLWTSDTQGDEAEAGEGGEN*

Amino acid sequence of GFP
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU31613
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee