cDNA Clone VACAMKL

• Synonyms: 1G5, MGC8407, CAMKV
• Plasmid: pGEX6P-3-VACAMKL
• Parent Plasmid: pGEX6P3
• Expressed: GST-VACAMKL
• Genbank: NM_024046.3 GI:83776595*
• Tag: GST
• Species: Human

Sequence of Insert

AGATCTATGCCGTTTGGGTGTGTGACTCTGGGCGACAAGAAGAACTATAA
CCAGCCATCGGAGGTGACTGACAGATATGATTTGGGACAGGTCATCAAGA
CTGAGGAGTTTTGTGAAATCTTCCGGGCCAAGGACAAGACGACAGGCAAG
CTGCACACCTGCAAGAAGTTCCAGAAGCGGGACGGCCGCAAGGTGCGGAA
AGCTGCCAAGAACGAGATAGGCATCCTCAAGATGGTGAAGCATCCCAACA
TCCTACAGCTGGTGGATGTGTTTGTGACCCGCAAGGAGTACTTTATCTTC
CTGGAGCTGGCCACGGGGAGGGAGGTGTTTGACTGGATCCTGGACCAGGG
CTACTACTCGGAGCGAGACACAAGCAACGTGGTACGGCAAGTCCTGGAGG
CCGTGGCCTATTTGCACTCACTCAAGATCGTGCACAGGAATCTCAAGCTG
GAGAACCTGGTTTACTACAACCGGCTGAAGAACTCGAAGATTGTCATCAG
TGACTTCCATCTGGCTAAGCTAGAAAATGGCCTCATCAAGGAGCCCTGTG
GGACCCCCGAGTATCTGGCCCCAGAGGTGGTAGGCCGGCAGCGGTATGGA
CGCCCTGTGGACTGCTGGGCCATTGGAGTCATCATGTACATCCTGCTTTC
AGGCAATCCACCTTTCTATGAGGAGGTGGAAGAAGATGATTATGAGAACC
ATGATAAGAATCTCTTCCGCAAGATCCTGGCTGGTGACTATGAGTTTGAC
TCTCCATATTGGGATGATATTTCGCAGGCAGCCAAAGACCTGGTCACAAG
GCTGATGGAGGTGGAGCAAGACCAGCGGATCACTGCAGAAGAGGCCATCT
CCCATGAGTGGATTTCTGGCAATGCTGCTTCTGATAAGAACATCAAGGAT
GGTGTCTGTGCCCAGATTGAAAAGAACTTTGCCAGGGCCAAGTGGAAGAA
GGCTGTCCGAGTGACCACCCTCATGAAACGGCTCCGGGCACCAGAGCAGT
CCAGCACGGCTGCAGCCCAGTCGGCCTCAGCCACAGACACTGCCACCCCC
GGGGCTGCAGGTGGGGCCACAGCTGCAGCTGCGAGTGGAGCTACCTCAGC
CCCTGAGGGTGATGCTGCTCGTGCTGCAAAGAGTGATAATGTGGCCCCCG
CAGACCGTAGTGCCACCCCAGCCACAGATGGAAGTGCCACCCCAGCCACT
GATGGCAGTGTCACCCCAGCCACCGATGGAAGCATCACTCCAGCCACTGA
TGGGAGTGTCACCCCAGCCACTGACAGGAGCGCTACTCCAGCCACTGATG
GGAGAGCCACACCAGCCACAGAAGAGAGCACTGTGCCCACCACCCAAAGC
AGTGCCATGCTGGCCACCAAGGCAGCTGCCACCCCTGAGCCGGCTATGGC
CCAGCCGGACAGCACAGCCCCAGAGGGCGCCACAGGCCAGGCTCCACCCT
CTAGTAAAGGGGAAGAGGCTGCTGGTTGTGCCCAGGAGTCTCAAAGGGAG
GAGGCCAGCTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMPFGCVTLGDKKNYNQPSE
VTDRYDLGQVIKTEEFCEIFRAKDKTTGKLHTCKKFQKRDGRKVRKAAKN
EIGILKMVKHPNILQLVDVFVTRKEYFIFLELATGREVFDWILDQGYYSE
RDTSNVVRQVLEAVAYLHSLKIVHRNLKLENLVYYNRLKNSKIVISDFHL
AKLENGLIKEPCGTPEYLAPEVVGRQRYGRPVDCWAIGVIMYILLSGNPP
FYEEVEEDDYENHDKNLFRKILAGDYEFDSPYWDDISQAAKDLVTRLMEV
EQDQRITAEEAISHEWISGNAASDKNIKDGVCAQIEKNFARAKWKKAVRV
TTLMKRLRAPEQSSTAAAQSASATDTATPGAAGGATAAAASGATSAPEGD
AARAAKSDNVAPADRSATPATDGSATPATDGSVTPATDGSITPATDGSVT
PATDRSATPATDGRATPATEESTVPTTQSSAMLATKAAATPEPAMAQPDS
TAPEGATGQAPPSSKGEEAAGCAQESQREEAS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36103
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence difference compared with NM_024046.3 CDS:; a1472g (Y491C - from EST, and present in several published seqs, including other species); Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 81113 Daltons