cDNA Clone USP30

• Plasmid: pFastbac GST USP30 (includes transmembrane domain)
• Parent Plasmid: pFastbac GST
• Expressed: GST-USP30
• Genbank: NM_032663.3 GI:164607166
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGCTGAGCTCCCGGGCCGAGGCGGCGATGACCGCGGCCGACAG
GGCCATCCAGCGCTTCCTGCGGACCGGGGCGGCCGTCAGATATAAAGTCA
TGAAGAACTGGGGAGTTATAGGTGGAATTGCTGCTGCTCTTGCAGCAGGA
ATATATGTTATTTGGGGTCCCATTACAGAAAGAAAGAAGCGTAGAAAAGG
GCTTGTGCCTGGCCTTGTTAATTTAGGGAACACCTGCTTCATGAACTCCC
TGCTACAAGGCCTGTCTGCCTGTCCTGCTTTCATCAGGTGGCTGGAAGAG
TTCACCTCCCAGTACTCCAGGGATCAGAAGGAGCCCCCCTCACACCAGTA
TTTATCCTTAACACTCTTGCACCTTCTGAAAGCCTTGTCCTGCCAAGAAG
TTACTGATGATGAGGTCTTAGATGCAAGCTGCTTGTTGGATGTCTTAAGA
ATGTACAGATGGCAGATCTCATCATTTGAAGAACAGGATGCTCACGAATT
ATTCCATGTCATTACCTCGTCATTGGAAGATGAGCGAGACCGCCAGCCTC
GGGTCACACATTTGTTTGATGTGCATTCCCTGGAGCAGCAGTCAGAAATA
ACTCCCAAACAAATTACCTGCCGCACAAGAGGGTCACCTCACCCTACATC
CAATCACTGGAAGTCTCAACATCCTTTTCATGGAAGACTCACTAGTAATA
TGGTCTGCAAACACTGTGAACACCAGAGTCCTGTTCGATTTGATACCTTT
GATAGCCTTTCACTAAGTATTCCAGCCGCCACATGGGGTCACCCATTGAC
CCTGGACCACTGCCTTCACCACTTCATCTCATCAGAATCAGTGCGGGATG
TTGTGTGTGACAACTGTACAAAGATTGAAGCCAAGGGAACGTTGAACGGG
GAAAAGGTGGAACACCAGAGGACCACTTTTGTTAAACAGTTAAAACTAGG
GAAGCTCCCTCAGTGTCTCTGCATCCACCTACAGCGGCTGAGCTGGTCCA
GCCACGGCACGCCTCTGAAGCGGCATGAGCACGTGCAGTTCAATGAGTTC
CTGATGATGGACATTTACAAGTACCACCTCCTTGGACATAAACCTAGTCA
ACACAACCCTAAACTGAACAAGAACCCAGGGCCTACACTGGAGCTGCAGG
ATGGGCCGGGAGCCCCCACACCAGTTCTGAATCAGCCAGGGGCCCCCAAA
ACACAGATTTTTATGAATGGCGCCTGCTCCCCATCTTTATTGCCAACGCT
GTCAGCGCCGATGCCCTTCCCTCTCCCAGTTGTTCCCGACTACAGCTCCT
CCACATACCTCTTCCGGCTGATGGCAGTTGTCGTCCACCATGGAGACATG
CACTCTGGACACTTTGTCACTTACCGACGGTCCCCACCTTCTGCCAGGAA
CCCTCTCTCAACTAGCAATCAGTGGCTGTGGGTCTCCGATGACACTGTCC
GCAAGGCCAGCCTGCAGGAGGTCCTGTCCTCCAGCGCCTACCTGCTGTTC
TACGAGCGCGTCCTTTCCAGGATGCAGCACCAGAGCCAGGAGTGCAAGTC
TGAAGAATGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMLSSRAEAAMTAADRAIQR
FLRTGAAVRYKVMKNWGVIGGIAAALAAGIYVIWGPITERKKRRKGLVPG
LVNLGNTCFMNSLLQGLSACPAFIRWLEEFTSQYSRDQKEPPSHQYLSLT
LLHLLKALSCQEVTDDEVLDASCLLDVLRMYRWQISSFEEQDAHELFHVI
TSSLEDERDRQPRVTHLFDVHSLEQQSEITPKQITCRTRGSPHPTSNHWK
SQHPFHGRLTSNMVCKHCEHQSPVRFDTFDSLSLSIPAATWGHPLTLDHC
LHHFISSESVRDVVCDNCTKIEAKGTLNGEKVEHQRTTFVKQLKLGKLPQ
CLCIHLQRLSWSSHGTPLKRHEHVQFNEFLMMDIYKYHLLGHKPSQHNPK
LNKNPGPTLELQDGPGAPTPVLNQPGAPKTQIFMNGACSPSLLPTLSAPM
PFPLPVVPDYSSSTYLFRLMAVVVHHGDMHSGHFVTYRRSPPSARNPLST
SNQWLWVSDDTVRKASLQEVLSSSAYLLFYERVLSRMQHQSQECKSEE*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU36244
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 85324 Daltons