cDNA Clone USP22

• Plasmid: pEBG6P-1 USP22
• Parent Plasmid: pEBG6P
• Expressed: GST USP22
• Genbank: NM_015276.1 GI:150010638
• Tag: GST
• Species: Human

Sequence of Insert

ggatccatggtgtcccggccagagcccgagggcgaggccatggacgccga
gctggcggtagcgccgccgggctgctcgcacctgggcagcttcaaggtgg
acaactggaagcagaacctgcgggccatctaccagtgcttcgtgtggagc
ggcacggctgaggcccgcaagcgcaaggccaagtcctgtatctgccatgt
ctgtggcgtccacctcaacaggctgcattcctgcctctactgtgtcttct
tcggctgtttcacaaagaagcatattcacgagcatgcgaaggcgaagcgg
cacaacctggccattgatctgatgtacggaggcatctactgttttctgtg
ccaggactacatctatgacaaagacatggaaataatcgccaaggaggagc
agcgaaaagcttggaaaatgcaaggcgttggagagaagttttcaacttgg
gaaccaaccaaacgggagcttgaactgctgaagcacaacccgaaaaggag
aaagatcacctcgaactgcaccataggtctgcgtgggctgatcaaccttg
ggaacacatgcttcatgaactgcatcgtgcaggccctgacccacacgcca
cttctgcgggacttcttcctgtctgacaggcaccgctgtgagatgcagag
ccccagctcctgtctggtctgtgagatgtcctcactgtttcaggagtttt
actctggacaccggtcccctcacatcccgtataagttgctgcacctggtg
tggacccacgcgaggcacctagcaggctacgagcagcaggacgcccacga
gttcctcatcgcggccctggacgtgctccaccgacactgcaaaggtgatg
acaatgggaagaaggccaacaaccccaaccactgcaactgcatcatagac
cagatcttcacaggcgggttgcagtcagacgtcacctgccaagtctgcca
tggagtctccaccaccatcgaccccttctgggacatcagcttggatctcc
ccggctcttccaccccattctggcccctgagcccagggagcgagggcaac
gtggtaaacggggaaagccacgtgtcgggaaccaccacgctcacggactg
cctgcgacgattcaccagaccagagcacttgggcagcagcgccaagatca
agtgcagcggttgccatagctaccaggagtccacaaagcagctcactatg
aagaaactgcccatcgtagcctgttttcatctcaaacgatttgaacactc
agccaagctgcggcggaagatcaccacgtatgtgtccttccccctggagc
tggacatgacccctttcatggcctccagcaaagagagcaggatgaatgga
cagtaccagcagcccacggacagtctcaacaatgacaacaagtattccct
gtttgctgttgttaaccatcaagggaccttggagagtggccactacacca
gctttatccggcagcacaaagaccagtggttcaagtgtgacgatgccatc
atcaccaaggccagcatcaaggacgtcctggacagcgaagggtacttgct
gttctatcacaaacagttcctggaatacgagtaggtcgactcgagcggcc
gc

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMVSRPEPEGEAMD
AELAVAPPGCSHLGSFKVDNWKQNLRAIYQCFVWSGTAEARKRKAKSCIC
HVCGVHLNRLHSCLYCVFFGCFTKKHIHEHAKAKRHNLAIDLMYGGIYCF
LCQDYIYDKDMEIIAKEEQRKAWKMQGVGEKFSTWEPTKRELELLKHNPK
RRKITSNCTIGLRGLINLGNTCFMNCIVQALTHTPLLRDFFLSDRHRCEM
QSPSSCLVCEMSSLFQEFYSGHRSPHIPYKLLHLVWTHARHLAGYEQQDA
HEFLIAALDVLHRHCKGDDNGKKANNPNHCNCIIDQIFTGGLQSDVTCQV
CHGVSTTIDPFWDISLDLPGSSTPFWPLSPGSEGNVVNGESHVSGTTTLT
DCLRRFTRPEHLGSSAKIKCSGCHSYQESTKQLTMKKLPIVACFHLKRFE
HSAKLRRKITTYVSFPLELDMTPFMASSKESRMNGQYQQPTDSLNNDNKY
SLFAVVNHQGTLESGHYTSFIRQHKDQWFKCDDAIITKASIKDVLDSEGY
LLFYHKQFLEYE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU36275
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express USP22 with N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 87376 Daltons