cDNA Clone USP30

• Synonyms: FLJ40511, MGC10702
• Plasmid: pGEX6P-USP30 57-end (lacks transmembrane domain)
• Parent Plasmid: pGEX6P3
• Expressed: GST-USP30 57-end
• Genbank: NM_032663.3 GI:164607166
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCACAGAAAGAAAGAAGCGTAGAAAAGGGCTTGTGCCTGGCCTTGT
TAATTTAGGGAACACCTGCTTCATGAACTCCCTGCTACAAGGCCTGTCTG
CCTGTCCTGCTTTCATCAGGTGGCTGGAAGAGTTCACCTCCCAGTACTCC
AGGGATCAGAAGGAGCCCCCCTCACACCAGTATTTATCCTTAACACTCTT
GCACCTTCTGAAAGCCTTGTCCTGCCAAGAAGTTACTGATGATGAGGTCT
TAGATGCAAGCTGCTTGTTGGATGTCTTAAGAATGTACAGATGGCAGATC
TCATCATTTGAAGAACAGGATGCTCACGAATTATTCCATGTCATTACCTC
GTCATTGGAAGATGAGCGAGACCGCCAGCCTCGGGTCACACATTTGTTTG
ATGTGCATTCCCTGGAGCAGCAGTCAGAAATAACTCCCAAACAAATTACC
TGCCGCACAAGAGGGTCACCTCACCCTACATCCAATCACTGGAAGTCTCA
ACATCCTTTTCATGGAAGACTCACTAGTAATATGGTCTGCAAACACTGTG
AACACCAGAGTCCTGTTCGATTTGATACCTTTGATAGCCTTTCACTAAGT
ATTCCAGCCGCCACATGGGGTCACCCATTGACCCTGGACCACTGCCTTCA
CCACTTCATCTCATCAGAATCAGTGCGGGATGTTGTGTGTGACAACTGTA
CAAAGATTGAAGCCAAGGGAACGTTGAACGGGGAAAAGGTGGAACACCAG
AGGACCACTTTTGTTAAACAGTTAAAACTAGGGAAGCTCCCTCAGTGTCT
CTGCATCCACCTACAGCGGCTGAGCTGGTCCAGCCACGGCACGCCTCTGA
AGCGGCATGAGCACGTGCAGTTCAATGAGTTCCTGATGATGGACATTTAC
AAGTACCACCTCCTTGGACATAAACCTAGTCAACACAACCCTAAACTGAA
CAAGAACCCAGGGCCTACACTGGAGCTGCAGGATGGGCCGGGAGCCCCCA
CACCAGTTCTGAATCAGCCAGGGGCCCCCAAAACACAGATTTTTATGAAT
GGCGCCTGCTCCCCATCTTTATTGCCAACGCTGTCAGCGCCGATGCCCTT
CCCTCTCCCAGTTGTTCCCGACTACAGCTCCTCCACATACCTCTTCCGGC
TGATGGCAGTTGTCGTCCACCATGGAGACATGCACTCTGGACACTTTGTC
ACTTACCGACGGTCCCCACCTTCTGCCAGGAACCCTCTCTCAACTAGCAA
TCAGTGGCTGTGGGTCTCCGATGACACTGTCCGCAAGGCCAGCCTGCAGG
AGGTCCTGTCCTCCAGCGCCTACCTGCTGTTCTACGAGCGCGTCCTTTCC
AGGATGCAGCACCAGAGCCAGGAGTGCAAGTCTGAAGAATGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSTERKKRRKGLVPGLVNLGN
TCFMNSLLQGLSACPAFIRWLEEFTSQYSRDQKEPPSHQYLSLTLLHLLK
ALSCQEVTDDEVLDASCLLDVLRMYRWQISSFEEQDAHELFHVITSSLED
ERDRQPRVTHLFDVHSLEQQSEITPKQITCRTRGSPHPTSNHWKSQHPFH
GRLTSNMVCKHCEHQSPVRFDTFDSLSLSIPAATWGHPLTLDHCLHHFIS
SESVRDVVCDNCTKIEAKGTLNGEKVEHQRTTFVKQLKLGKLPQCLCIHL
QRLSWSSHGTPLKRHEHVQFNEFLMMDIYKYHLLGHKPSQHNPKLNKNPG
PTLELQDGPGAPTPVLNQPGAPKTQIFMNGACSPSLLPTLSAPMPFPLPV
VPDYSSSTYLFRLMAVVVHHGDMHSGHFVTYRRSPPSARNPLSTSNQWLW
VSDDTVRKASLQEVLSSSAYLLFYERVLSRMQHQSQECKSEE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36294
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 79407 Daltons