cDNA Clone USP27X

• Synonyms: USP22L, USP27
• Plasmid: pEBG6P-USP27X
• Parent Plasmid: pEBG6P
• Expressed: GST-USP27X
• Genbank: NM_001145073.1 GI:223005905
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTGTAAGGACTATGTATATGACAAAGACATTGAGCAAATTGC
CAAAGAAGAGCAAGGAGAAGCTTTGAAATTACAAGCCTCCACCTCAACAG
AGGTTTCTCACCAGCAGTGTTCAGTGCCAGGCCTTGGTGAGAAATTCCCA
ACCTGGGAAACAACCAAACCAGAATTAGAACTGCTGGGGCACAACCCGAG
GAGAAGAAGAATCACCTCCAGCTTTACGATCGGTTTAAGAGGACTCATCA
ATCTTGGCAACACGTGCTTTATGAACTGCATTGTCCAGGCCCTCACCCAC
ACGCCGATACTGAGAGATTTCTTTCTCTCTGACAGGCACCGATGTGAGAT
GCCGAGTCCCGAGTTGTGTCTGGTCTGTGAGATGTCGTCGCTGTTTCGGG
AGTTGTATTCTGGAAACCCGTCTCCTCATGTGCCCTATAAGTTACTGCAC
CTGGTGTGGATACATGCCCGCCATTTAGCAGGGTACAGGCAACAGGATGC
CCACGAGTTCCTCATTGCAGCGTTAGATGTCCTGCACAGGCACTGCAAAG
GTGATGATGTCGGGAAGGCGGCCAACAATCCCAACCACTGTAACTGCATC
ATAGACCAAATCTTCACAGGTGGCCTGCAGTCTGATGTCACCTGTCAAGC
CTGCCATGGCGTCTCCACCACGATAGACCCATGCTGGGACATTAGTTTGG
ACTTGCCTGGCTCTTGCACCTCCTTCTGGCCCATGAGCCCAGGGAGGGAG
AGCAGTGTGAACGGGGAAAGCCACATACCAGGAATCACCACCCTCACGGA
CTGCTTGCGGAGGTTTACGAGGCCAGAGCACTTAGGAAGCAGTGCCAAAA
TCAAATGTGGTAGTTGCCAAAGCTACCAGGAATCTACCAAACAGCTCACA
ATGAATAAATTACCTGTCGTTGCCTGTTTTCATTTCAAACGGTTTGAACA
TTCAGCGAAACAGAGGCGCAAGATCACTACATACATTTCCTTTCCTCTGG
AGCTGGATATGACGCCGTTTATGGCCTCAAGTAAAGAGAGCAGAATGAAT
GGACAATTGCAGCTGCCAACCAATAGTGGAAACAACGAAAATAAGTATTC
CTTGTTTGCTGTGGTTAATCACCAAGGAACCTTGGAGAGTGGCCACTATA
CCAGCTTCATCCGGCACCACAAGGACCAGTGGTTCAAGTGTGATGATGCC
GTCATCACTAAGGCCAGTATTAAGGACGTACTGGACAGTGAAGGGTATTT
ACTGTTCTATCACAAACAGGTGCTAGAACATGAGTCAGAAAAAGTGAAAG
AAATGAACACACAAGCCTACTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMCKDYVYDKDIEQ
IAKEEQGEALKLQASTSTEVSHQQCSVPGLGEKFPTWETTKPELELLGHN
PRRRRITSSFTIGLRGLINLGNTCFMNCIVQALTHTPILRDFFLSDRHRC
EMPSPELCLVCEMSSLFRELYSGNPSPHVPYKLLHLVWIHARHLAGYRQQ
DAHEFLIAALDVLHRHCKGDDVGKAANNPNHCNCIIDQIFTGGLQSDVTC
QACHGVSTTIDPCWDISLDLPGSCTSFWPMSPGRESSVNGESHIPGITTL
TDCLRRFTRPEHLGSSAKIKCGSCQSYQESTKQLTMNKLPVVACFHFKRF
EHSAKQRRKITTYISFPLELDMTPFMASSKESRMNGQLQLPTNSGNNENK
YSLFAVVNHQGTLESGHYTSFIRHHKDQWFKCDDAVITKASIKDVLDSEG
YLLFYHKQVLEHESEKVKEMNTQAY*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU36342
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express USP27X with N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 77046 Daltons