cDNA Clone USP51

• Plasmid: pEBG6P-1 USP51
• Parent Plasmid: pEBG6P
• Expressed: GST-USP51
• Genbank: NM_201286.3
• Tag: GST

Sequence of Insert

AGATCTATGGCCCAGGTTCGAGAAACTTCTTTGCCCTCCGGCTCTGGGGTCCGCTGGATCTCCGGAGGTGGGGGAGGAGCCTCTCCTGAGGAGGCGGTTGAGAAGGCGGGGAAAATGGAGGAGGCGGCGGCGGGGGCTACGAAGGCGTCTTCGAGACGTGAAGCCGAGGAGATGAAGCTGGAGCCATTACAAGAGCGTGAGCCCGCGCCGGAGGAGAACTTGACGTGGAGCAGCAGCGGCGGCGACGAGAAGGTGCTCCCTTCAATCCCCCTTCGCTGTCACAGCAGCTCCTCGCCCGTTTGCCCGCGCCGCAAGCCCCGCCCTCGGCCCCAGCCCCGGGCCCGCTCCCGCAGCCAGCCTGGGCTCTCGGCCCCACCCCCGCCTCCAGCCCGGCCCCCGCCCCCGCCGCCACCCCCGCCCCCACCCGCACCGCGGCCCAGGGCCTGGCGTGGATCCCGGCGCAGATCCCGGCCTGGGTCCAGGCCTCAGACACGGAGAAGCTGCTCTGGTGACCTAGACGGGTCGGGGGATCCTGGCGGCTTAGGGGACTGGTTGCTGGAGGTCGAGTTTGGTCAGGGTCCCACAGGCTGCTCTCATGTGGAGAGCTTTAAAGTAGGTAAGAACTGGCAGAAGAACCTGAGGTTGATCTACCAGCGTTTCGTTTGGAGTGGGACCCCAGAGACTAGGAAACGTAAAGCAAAGTCATGCATCTGTCACGTATGTAGTACCCATATGAACAGACTCCACTCTTGTCTCTCCTGTGTCTTTTTTGGCTGCTTCACTGAGAAACATATTCACAAACATGCAGAAACAAAGCAGCACCATTTAGCTGTAGACCTTTATCATGGGGTCATATATTGCTTCATGTGTAAGGATTATGTATATGACAAAGACATAGAACAGATTGCCAAAGAAACAAAAGAAAAAATTTTGAGATTATTAACTTCCACCTCAACAGATGTTTCTCATCAACAGTTTATGACATCAGGGTTTGAAGACAAGCAATCAACCTGTGAGACAAAGGAACAGGAGCCAAAATTGGTGAAACCCAAGAAAAAGAGAAGAAAAAAGTCAGTCTATACTGTAGGCCTGAGAGGGCTAATCAATCTTGGGAACACTTGTTTTATGAATTGTATTGTCCAGGCACTTACCCATATTCCTCTACTGAAAGATTTCTTCCTCTCTGACAAGCACAAATGTATAATGACAAGCCCCAGCTTGTGTCTGGTCTGTGAAATGTCTTCGCTTTTTCATGCTATGTACTCTGGGAGCCGAACTCCTCACATTCCCTATAAGTTACTGCATCTGATATGGATCCATGCAGAACATTTAGCAGGGTACAGGCAGCAGGATGCCCATGAGTTCCTTATTGCAATATTAGACGTGCTACATAGACACAGCAAAGATGATAGTGGTGGGCAGGAGGCCAATAACCCCAACTGCTGTAACTGCATCATAGACCAAATCTTTACAGGTGGCCTGCAATCAGATGTCACATGTCAAGCCTGCCATAGTGTTTCTACCACCATAGACCCATGCTGGGACATCAGTTTGGACTTGCCTGGCTCTTGTGCCACATTCGATTCCCAGAACCCAGAGAGGGCTGACAGCACAGTGAGCAGGGATGACCACATACCAGGAATCCCCTCACTTACAGACTGTCTACAGTGGTTTACAAGGCCAGAGCACCTAGGAAGCAGTGCCAAAATCAAATGCAATAGTTGCCAAAGCTACCAGGAGTCTACTAAACAGCTCACAATGAAAAAATTACCCATTGTGGCTTGTTTTCATCTCAAGCGGTTTGAGCACGTAGGCAAACAGAGGCGAAAGATTAATACCTTTATCTCCTTTCCCTTGGAGCTGGACATGACTCCGTTTTTGGCCTCTACTAAAGAGAGCAGAATGAAAGAAGGCCAGCCACCAACAGATTGTGTGCCCAACGAGAATAAGTATTCCTTGTTTGCAGTGATTAATCACCATGGAACTTTGGAAAGTGGCCACTATACCAGCTTCATCCGGCAACAAAAGGACCAGTGGTTCAGCTGTGATGATGCCATCATCACCAAGGCTACCATTGAGGACTTACTCTACAGTGAAGGGTATTTACTGTTCTATCACAAACAGGGTCTAGAGAAAGACTAGGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMAQVRETSLPSGSGVRWISGGGGGASPEEAVEKAGKMEEAAAGATKASSRREAEEMKLEPLQE
REPAPEENLTWSSSGGDEKVLPSIPLRCHSSSSPVCPRRKPRPRPQPRARSRSQPGLSAPPPPPARPPPPPPPPPPPAPRPRAWRGSRRRSRPGSRPQTR
RSCSGDLDGSGDPGGLGDWLLEVEFGQGPTGCSHVESFKVGKNWQKNLRLIYQRFVWSGTPETRKRKAKSCICHVCSTHMNRLHSCLSCVFFGCFTEKHI
HKHAETKQHHLAVDLYHGVIYCFMCKDYVYDKDIEQIAKETKEKILRLLTSTSTDVSHQQFMTSGFEDKQSTCETKEQEPKLVKPKKKRRKKSVYTVGLR
GLINLGNTCFMNCIVQALTHIPLLKDFFLSDKHKCIMTSPSLCLVCEMSSLFHAMYSGSRTPHIPYKLLHLIWIHAEHLAGYRQQDAHEFLIAILDVLHR
HSKDDSGGQEANNPNCCNCIIDQIFTGGLQSDVTCQACHSVSTTIDPCWDISLDLPGSCATFDSQNPERADSTVSRDDHIPGIPSLTDCLQWFTRPEHLG
SSAKIKCNSCQSYQESTKQLTMKKLPIVACFHLKRFEHVGKQRRKINTFISFPLELDMTPFLASTKESRMKEGQPPTDCVPNENKYSLFAVINHHGTLES
GHYTSFIRQQKDQWFSCDDAIITKATIEDLLYSEGYLLFYHKQGLEKD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU36621
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Two silent changes compared with reference sequence: t1803c (silent at His601) and t1935c (silent at Asn645)
• Low Temp: No