cDNA Clone UBE2V1

• Plasmid: pGEX6P-1-UBE2V1
• Parent Plasmid: pGEX6P1
• Expressed: GST-UBE2V1
• Genbank: NP_954580
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCGCGGGCG
CCGAGGACTGGCCGGGCCAGCAGCTGGAGCTGGACGAGGACGAGGCGTCT
TGTTGCCGCTGGGGCGCGCAGCACGCCGGGGCCCGCGAGCTGGCTGCGCT
CTACTCGCCAGGCAAGCGCCTCCAGGAGTGGTGCTCTGTGATCCTGTGCT
TCAGCCTCATCGCCCACAACCTGGTCCATCTCCTGCTGCTGGCCCGCTGG
GAGGACACACCCCTCGTCATACTCGGTGTTGTTGCAGGGGCTCTCATTGC
TGACTTCTTGTCTGGCCTGGTACACTGGGGTGCTGACACATGGGGCTCTG
TGGAGCTGCCCATTGTGGGGAAGGCTTTCATCCGACCCTTCCGGGAGCAC
CACATTGACCCGACAGCTATCACACGGCACGACTTCATCGAGACCAACGG
GGACAACTGCCTGGTGACACTGCTGCCGCTGCTAAACATGGCCTACAAGT
TCCGCACCCACAGCCCTGAAGCCCTGGAGCAGCTATACCCCTGGGAGTGC
TTCGTCTTCTGCCTGATCATCTTCGGCACCTTCACCAACCAGATCCACAA
GTGGTCGCACACGTACTTTGGGCTGCCACGCTGGGTCACCCTCCTGCAGG
ACTGGCATGTCATCCTGCCACGTAAACACCATCGCATCCACCACGTCTCA
CCCCACGAGACCTACTTCTGCATCACCACAGGCTGGCTCAACTACCCTCT
GGAGAAGATAGGCTTCTGGCGACGCCTGGAGGACCTCATCCAGGGCCTGA
CGGGCGAGAAGCCTCGGGCAGATGACATGAAATGGGCCCAGAAGATCAAA
TAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAGAEDWPGQQLELDEDEAS
CCRWGAQHAGARELAALYSPGKRLQEWCSVILCFSLIAHNLVHLLLLARW
EDTPLVILGVVAGALIADFLSGLVHWGADTWGSVELPIVGKAFIRPFREH
HIDPTAITRHDFIETNGDNCLVTLLPLLNMAYKFRTHSPEALEQLYPWEC
FVFCLIIFGTFTNQIHKWSHTYFGLPRWVTLLQDWHVILPRKHHRIHHVS
PHETYFCITTGWLNYPLEKIGFWRRLEDLIQGLTGEKPRADDMKWAQKIK
*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4206
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GST.