cDNA Clone UBCH5a

• Plasmid: pCMV5-HA UBE2D1/UBCH5a C85A
• Parent Plasmid: pCMV5
• Expressed: HA-UBE2D1/UBCH5a C85A
• Genbank: NM_003338
• Tag: HA
• Species: Human

Sequence of Insert

GAATTGGCCACCATGTACCCATACGATGTGCCAGATTACGCCGGATCCGC
GCTGAAGAGGATTCAGAAAGAATTGAGTGATCTACAGCGCGATCCACCTG
CTCACTGTTCAGCTGGACCTGTGGGAGATGACTTGTTCCACTGGCAAGCC
ACTATTATGGGGCCTCCTGATAGCGCATATCAAGGTGGAGTCTTCTTTCT
CACTGTACATTTTCCGACAGATTATCCTTTTAAACCACCAAAGATTGCTT
TCACAACAAAAATTTACCATCCAAACATAAACAGTAATGGAAGTATTGCT
CTCGATATTCTGAGGTCACAATGGTCACCAGCTCTGACTGTATCAAAAGT
TTTATTGTCCATATGTTCTCTACTTTGTGATCCTAATCCAGATGACCCCT
TAGTACCAGATATTGCACAAATCTATAAATCAGACAAAGAAAAATACAAC
AGACATGCAAGAGAATGGACTCAGAAATATGCAATGTAAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSALKRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMG
PPDSAYQGGVFFLTVHFPTDYPFKPPKIAFTTKIYHPNINSNGSIALDIL
RSQWSPALTVSKVLLSICSLLCDPNPDDPLVPDIAQIYKSDKEKYNRHAR
EWTQKYAM*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4221
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal HA tag.