cDNA Clone UBCH5b

• Plasmid: pET28a(+) UBE2D2 (UBCH5b)
• Parent Plasmid: pET28a original
• Expressed: His-UBE2D2 (UBCH5b)
• Genbank: NM_003339
• Tag: 6His
• Species: Human

Sequence of Insert

ATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTGCCGCG
CGGCAGCCATATGGCTAGCATGACTGGTGGACAGCAAATGGGTCGCGGAT
CCGCTCTGAAGAGAATCCACAAGGAATTGAATGATCTGGCACGGGACCCT
CCAGCACAGTGTTCAGCAGGTCCTGTTGGAGATGATATGTTCCATTGGCA
AGCTACAATAATGGGGCCAAATGACAGTCCCTATCAGGGTGGAGTATTTT
TCTTGACAATTCATTTCCCAACAGATTACCCCTTCAAACCACCTAAGGTT
GCATTTACAACAAGAATTTATCATCCAAATATTAACAGTAATGGCAGCAT
TTGTCTTGATATTCTACGATCACAGTGGTCTCCAGCACTAACTATTTCAA
AAGTACTCTTGTCCATCTGTTCTCTGTTGTGTGATCCCAATCCAGATGAT
CCTTTAGTGCCTGAGATTGCTCGGATCTACAAAACAGATAGAGAAAAGTA
CAACAGAATAGCTCGGGAATGGACTCAGAAGTATGCGATGTAAGCGGCCG
C

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSALKRIHKELNDLARDP
PAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKV
AFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDD
PLVPEIARIYKTDREKYNRIAREWTQKYAM*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU4316
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term 6H.