cDNA Clone CDC34

• Synonyms: UBCH3, UBC3, UBE2R1
• Plasmid: pET28a(+) CDC34
• Parent Plasmid: pET28a original
• Expressed: 6His-CDC34
• Genbank: NM_004359
• Tag: 6His
• Species: Human

Sequence of Insert

ATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTGCCGCG
CGGCAGCCATATGGCTAGCATGACTGGTGGACAGCAAATGGGTCGCGGAT
CCGCTCGGCCGCTAGTGCCCAGCTCGCAGAAGGCGCTGCTGCTGGAGCTC
AAGGGGCTGCAGGAAGAGCCGGTCGAGGGATTCCGCGTGACACTGGTGGA
CGAGGGCGATCTATACAACTGGGAGGTGGCCATCTTCGGGCCCCCCAACA
CCTACTACGAGGGCGGCTACTTCAAGGCGCGCCTCAAGTTCCCCATCGAC
TACCCATACTCTCCACCAGCCTTTCGGTTCCTGACCAAGATGTGGCACCC
TAACATCTACGAGACGGGGGACGTGTGTATCTCCATCCTCCACCCGCCGG
TGGACGACCCCCAGAGCGGGGAGCTGCCCTCAGAGAGGTGGAACCCCACG
CAGAACGTCAGGACCATTCTCCTGAGTGTGATCTCCCTCCTGAACGAGCC
CAACACCTTCTCGCCCGCAAACGTGGACGCCTCCGTGATGTACAGGAAGT
GGAAAGAGAGCAAGGGGAAGGATCGGGAGTACACAGACATCATCCGGAAG
CAGGTCCTGGGGACCAAGGTGGACGCGGAGCGTGACGGCGTGAAGGTGCC
CACCACGCTGGCCGAGTACTGCGTGAAGACCAAGGCGCCGGCGCCCGACG
AGGGCTCAGACCTCTTCTACGACGACTACTACGAGGACGGCGAGGTGGAG
GAGGAGGCCGACAGCTGCTTCGGGGACGATGAGGATGACTCTGGCACGGA
GGAGTCCTGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSARPLVPSSQKALLLEL
KGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPID
YPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPT
QNVRTILLSVISLLNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRK
QVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVE
EEADSCFGDDEDDSGTEES*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU4317
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term 6H. Ala35 of fusion product = Ala2 of coding sequence