cDNA Clone WNK2

• Plasmid: pFBHTb WNK2 166-489
• Parent Plasmid: pFastBac HTb
• Expressed: His-WNK2 166-489
• Genbank: NM_006648
• Tag: 6His
• Species: Human

Sequence of Insert

ATGTCGTACTACCATCACCATCACCATCACGATTACGATATCCCAACGAC
CGAAAACCTGTATTTTCAGGGCGCCATGGGATCCGGGCGCACTCGCCGGG
ACGAGCCCGAAGAGGAGGAGGACGACGAGGACGACCTCAAGGCCGTGGCC
ACCTCTCTGGACGGCCGCTTCCTCAAGTTCGACATCGAGCTGGGCCGCGG
TTCCTTCAAGACGGTCTACAAGGGGCTGGACACGGAGACCTGGGTGGAGG
TGGCCTGGTGTGAGCTGCAGGACCGGAAGCTCACCAAGCTGGAGCGGCAG
CGGTTCAAGGAAGAGGCTGAGATGCTGAAAGGCCTGCAGCACCCCAACAT
CGTGCGCTTCTACGACTTCTGGGAGTCCAGCGCCAAGGGCAAGCGGTGCA
TTGTGCTGGTGACGGAGCTGATGACCTCAGGGACGCTGAAGACATACCTG
AAGCGGTTCAAGGTGATGAAGCCCAAGGTTCTCCGCAGCTGGTGCCGGCA
GATCCTGAAGGGCCTGCTGTTCCTGCACACAAGGACGCCACCCATCATCC
ACCGAGACCTGAAATGTGACAATATTTTCATCACCGGACCAACTGGGTCT
GTGAAGATTGGCGACTTGGGCCTGGCCACTCTGAAAAGAGCGTCATTTGC
CAAAAGTGTGATAGGTACTCCCGAGTTCATGGCGCCCGAGATGTACGAGG
AGCACTACGATGAGTCCGTGGACGTCTATGCCTTTGGGATGTGCATGCTG
GAGATGGCCACCTCGGAGTACCCCTACTCGGAGTGCCAGAATGCGGCCCA
GATCTACCGCAAGGTCACCTGTGGTATCAAGCCGGCCAGCTTTGAGAAAG
TGCACGATCCTGAAATCAAGGAGATTATTGGGGAGTGTATCTGCAAAAAC
AAGGAGGAAAGGTACGAGATCAAAGACCTGCTGAGCCACGCCTTCTTCGC
AGAGGACACAGGCGTGAGGGTGGAGCTCGCGGAGGAGGACCACGGCAGGA
AGTCCACCATCGCCCTGAGGCTCTGGGTGGAAGACCCCAAGAAACTGAAG
GGAAAGTGAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSGRTRRDEPEEEEDDEDDLKAVA
TSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQ
RFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYL
KRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGS
VKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCML
EMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKN
KEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLK
GK*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU4404
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression with N term 6H tag.; ; All WNK plasmids MUST be grown at 30 degrees C or less to prevent recombination.