cDNA Clone WNK2

• Plasmid: pCMV5-HA-1 WNK2 166-489
• Parent Plasmid: pCMV5
• Expressed: HA-WNK2 166-489
• Genbank: NM_006648
• Tag: HA
• Species: Human

Sequence of Insert

GCCACCATGTACCCATACGATGTGCCAGATTACGCCGGATCCGGGCGCAC
TCGCCGGGACGAGCCCGAAGAGGAGGAGGACGACGAGGACGACCTCAAGG
CCGTGGCCACCTCTCTGGACGGCCGCTTCCTCAAGTTCGACATCGAGCTG
GGCCGCGGTTCCTTCAAGACGGTCTACAAGGGGCTGGACACGGAGACCTG
GGTGGAGGTGGCCTGGTGTGAGCTGCAGGACCGGAAGCTCACCAAGCTGG
AGCGGCAGCGGTTCAAGGAAGAGGCTGAGATGCTGAAAGGCCTGCAGCAC
CCCAACATCGTGCGCTTCTACGACTTCTGGGAGTCCAGCGCCAAGGGCAA
GCGGTGCATTGTGCTGGTGACGGAGCTGATGACCTCAGGGACGCTGAAGA
CATACCTGAAGCGGTTCAAGGTGATGAAGCCCAAGGTTCTCCGCAGCTGG
TGCCGGCAGATCCTGAAGGGCCTGCTGTTCCTGCACACAAGGACGCCACC
CATCATCCACCGAGACCTGAAATGTGACAATATTTTCATCACCGGACCAA
CTGGGTCTGTGAAGATTGGCGACTTGGGCCTGGCCACTCTGAAAAGAGCG
TCATTTGCCAAAAGTGTGATAGGTACTCCCGAGTTCATGGCGCCCGAGAT
GTACGAGGAGCACTACGATGAGTCCGTGGACGTCTATGCCTTTGGGATGT
GCATGCTGGAGATGGCCACCTCGGAGTACCCCTACTCGGAGTGCCAGAAT
GCGGCCCAGATCTACCGCAAGGTCACCTGTGGTATCAAGCCGGCCAGCTT
TGAGAAAGTGCACGATCCTGAAATCAAGGAGATTATTGGGGAGTGTATCT
GCAAAAACAAGGAGGAAAGGTACGAGATCAAAGACCTGCTGAGCCACGCC
TTCTTCGCAGAGGACACAGGCGTGAGGGTGGAGCTCGCGGAGGAGGACCA
CGGCAGGAAGTCCACCATCGCCCTGAGGCTCTGGGTGGAAGACCCCAAGA
AACTGAAGGGAAAGTGAGCGGCCGCG

Amino Acid Sequence

MYPYDVPDYAGSGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGR
GSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPN
IVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCR
QILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASF
AKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAA
QIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFF
AEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4405
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term HA tag.; ; All WNK plasmids MUST be grown at 30 degrees C or less to prevent recombination.