cDNA Clone WNK2

• Plasmid: pEBG6P-1 WNK2 166-489
• Parent Plasmid: pCMV5
• Expressed: GST-WNK2 166-489
• Genbank: NM_006648
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCGGGCGCACTCGCCGGGACGAGCCCGAAGAGGAGGAGGAC
GACGAGGACGACCTCAAGGCCGTGGCCACCTCTCTGGACGGCCGCTTCCT
CAAGTTCGACATCGAGCTGGGCCGCGGTTCCTTCAAGACGGTCTACAAGG
GGCTGGACACGGAGACCTGGGTGGAGGTGGCCTGGTGTGAGCTGCAGGAC
CGGAAGCTCACCAAGCTGGAGCGGCAGCGGTTCAAGGAAGAGGCTGAGAT
GCTGAAAGGCCTGCAGCACCCCAACATCGTGCGCTTCTACGACTTCTGGG
AGTCCAGCGCCAAGGGCAAGCGGTGCATTGTGCTGGTGACGGAGCTGATG
ACCTCAGGGACGCTGAAGACATACCTGAAGCGGTTCAAGGTGATGAAGCC
CAAGGTTCTCCGCAGCTGGTGCCGGCAGATCCTGAAGGGCCTGCTGTTCC
TGCACACAAGGACGCCACCCATCATCCACCGAGACCTGAAATGTGACAAT
ATTTTCATCACCGGACCAACTGGGTCTGTGAAGATTGGCGACTTGGGCCT
GGCCACTCTGAAAAGAGCGTCATTTGCCAAAAGTGTGATAGGTACTCCCG
AGTTCATGGCGCCCGAGATGTACGAGGAGCACTACGATGAGTCCGTGGAC
GTCTATGCCTTTGGGATGTGCATGCTGGAGATGGCCACCTCGGAGTACCC
CTACTCGGAGTGCCAGAATGCGGCCCAGATCTACCGCAAGGTCACCTGTG
GTATCAAGCCGGCCAGCTTTGAGAAAGTGCACGATCCTGAAATCAAGGAG
ATTATTGGGGAGTGTATCTGCAAAAACAAGGAGGAAAGGTACGAGATCAA
AGACCTGCTGAGCCACGCCTTCTTCGCAGAGGACACAGGCGTGAGGGTGG
AGCTCGCGGAGGAGGACCACGGCAGGAAGTCCACCATCGCCCTGAGGCTC
TGGGTGGAAGACCCCAAGAAACTGAAGGGAAAGTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSGRTRRDEPEEEED
DEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQD
RKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELM
TSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDN
IFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVD
VYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKE
IIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRL
WVEDPKKLKGK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4407
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term GST tag.; ; All WNK plasmids MUST be grown at 30 degrees C or less to prevent recombination.