cDNA Clone WNK3

• Plasmid: pFBHTa WNK3 1-434
• Parent Plasmid: pSC-A
• Expressed: His-WNK3 1-434
• Genbank: NP_020922
• Tag: 6His
• Species: Human

Sequence of Insert

ATGTCGTACTACCATCACCATCACCATCACGATTACGATATCCCAACGAC
CGAAAACCTGTATTTTCAGGGCGCCATGGATCCGGAATTCATGGCCACTG
ATTCAGGGGATCCAGCCAGCACAGAAGATTCTGAGAAACCTGATGGAATT
TCATTTGAAAACAGAGTTCCCCAGGTCGCTGCAACTTTGACAGTAGAAGC
TAGACTAAAGGAGAAAAACAGTACCTTCTCTGCTTCTGGGGAAACTGTAG
AAAGGAAGAGATTTTTCCGAAAGAGTGTTGAAATGACGGAAGATGACAAA
GTTGCCGAATCATCCCCCAAAGATGAGAGAATTAAGGCTGCAATGAATAT
TCCAAGAGTAGATAAGCTTCCTTCAAATGTGTTGAGAGGTGGACAAGAAG
TTAAATATGAACAGTGTTCAAAGTCAACCTCAGAAATCTCAAAAGATTGT
TTCAAGGAGAAAAATGAAAAGGAAATGGAAGAAGAAGCAGAAATGAAGGC
TGTAGCTACTTCTCCTAGTGGCAGATTCCTGAAATTTGACATAGAACTAG
GAAGAGGAGCATTTAAAACAGTATATAAAGGACTGGACACTGAAACATGG
GTTGAGGTTGCTTGGTGTGAGCTGCAGGACCGAAAGTTAACCAAAGCTGA
GCAGCAAAGATTCAAGGAAGAAGCAGAGATGTTGAAGGGTCTCCAGCACC
CCAATATAGTTCGATTTTATGATTCCTGGGAATCTATATTAAAAGGAAAG
AAATGTATTGTATTAGTGACTGAACTAATGACATCTGGGACCTTAAAGAC
GTACTTAAAACGATTTAAAGTCATGAAACCAAAGGTCTTAAGGAGCTGGT
GCAGGCAAATTTTAAAGGGGTTGCAGTTCTTGCACACTAGGACTCCTCCT
ATTATTCACCGGGATCTGAAGTGTGACAATATTTTCATCACGGGACCCAC
TGGATCTGTGAAGATTGGTGATCTAGGATTAGCCACCTTAATGCGTACCT
CATTTGCTAAGAGTGTCATTGGAACTCCTGAGTTTATGGCTCCAGAGATG
TATGAAGAACACTATGATGAATCCGTAGATGTTTATGCTTTTGGAATGTG
TATGCTGGAAATGGCCACATCGGAGTATCCTTATTCTGAGTGTCAGAATG
CAGCTCAAATATACCGGAAAGTAACTAGTGGCATAAAACCAGCCAGCTTC
AATAAAGTCACTGATCCTGAAGTCAAAGAAATCATTGAAGGATGTATTCG
TCAAAACAAATCTGAAAGGTTGTCTATCAGGGACCTATTAAACCATGCAT
TTTTTGCTGAGGATACAGGACTGAGGGTGGAGTTAGCAGAAGAAGATGAT
TGCTCAAATTCATCCCTTGCTTTAAGACTCTGGGTTGAAGACTGAGCGGC
CGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFMATDSGDPASTEDSEKPDGI
SFENRVPQVAATLTVEARLKEKNSTFSASGETVERKRFFRKSVEMTEDDK
VAESSPKDERIKAAMNIPRVDKLPSNVLRGGQEVKYEQCSKSTSEISKDC
FKEKNEKEMEEEAEMKAVATSPSGRFLKFDIELGRGAFKTVYKGLDTETW
VEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSWESILKGK
KCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHTRTPP
IIHRDLKCDNIFITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFMAPEM
YEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGIKPASF
NKVTDPEVKEIIEGCIRQNKSERLSIRDLLNHAFFAEDTGLRVELAEEDD
CSNSSLALRLWVED*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU4408
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression with N term 6H tag.; ; All WNK plasmids MUST be grown at 30 degrees C or less to prevent recombination.