cDNA Clone WNK3

• Plasmid: pGEX6P-1-WNK3 1-434
• Parent Plasmid: pGEX6P1
• Expressed: GST-WNK3 1-434
• Genbank: NP_020922
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCGGAAT
TCATGGCCACTGATTCAGGGGATCCAGCCAGCACAGAAGATTCTGAGAAA
CCTGATGGAATTTCATTTGAAAACAGAGTTCCCCAGGTCGCTGCAACTTT
GACAGTAGAAGCTAGACTAAAGGAGAAAAACAGTACCTTCTCTGCTTCTG
GGGAAACTGTAGAAAGGAAGAGATTTTTCCGAAAGAGTGTTGAAATGACG
GAAGATGACAAAGTTGCCGAATCATCCCCCAAAGATGAGAGAATTAAGGC
TGCAATGAATATTCCAAGAGTAGATAAGCTTCCTTCAAATGTGTTGAGAG
GTGGACAAGAAGTTAAATATGAACAGTGTTCAAAGTCAACCTCAGAAATC
TCAAAAGATTGTTTCAAGGAGAAAAATGAAAAGGAAATGGAAGAAGAAGC
AGAAATGAAGGCTGTAGCTACTTCTCCTAGTGGCAGATTCCTGAAATTTG
ACATAGAACTAGGAAGAGGAGCATTTAAAACAGTATATAAAGGACTGGAC
ACTGAAACATGGGTTGAGGTTGCTTGGTGTGAGCTGCAGGACCGAAAGTT
AACCAAAGCTGAGCAGCAAAGATTCAAGGAAGAAGCAGAGATGTTGAAGG
GTCTCCAGCACCCCAATATAGTTCGATTTTATGATTCCTGGGAATCTATA
TTAAAAGGAAAGAAATGTATTGTATTAGTGACTGAACTAATGACATCTGG
GACCTTAAAGACGTACTTAAAACGATTTAAAGTCATGAAACCAAAGGTCT
TAAGGAGCTGGTGCAGGCAAATTTTAAAGGGGTTGCAGTTCTTGCACACT
AGGACTCCTCCTATTATTCACCGGGATCTGAAGTGTGACAATATTTTCAT
CACGGGACCCACTGGATCTGTGAAGATTGGTGATCTAGGATTAGCCACCT
TAATGCGTACCTCATTTGCTAAGAGTGTCATTGGAACTCCTGAGTTTATG
GCTCCAGAGATGTATGAAGAACACTATGATGAATCCGTAGATGTTTATGC
TTTTGGAATGTGTATGCTGGAAATGGCCACATCGGAGTATCCTTATTCTG
AGTGTCAGAATGCAGCTCAAATATACCGGAAAGTAACTAGTGGCATAAAA
CCAGCCAGCTTCAATAAAGTCACTGATCCTGAAGTCAAAGAAATCATTGA
AGGATGTATTCGTCAAAACAAATCTGAAAGGTTGTCTATCAGGGACCTAT
TAAACCATGCATTTTTTGCTGAGGATACAGGACTGAGGGTGGAGTTAGCA
GAAGAAGATGATTGCTCAAATTCATCCCTTGCTTTAAGACTCTGGGTTGA
AGACTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMATDSGDPASTEDSEK
PDGISFENRVPQVAATLTVEARLKEKNSTFSASGETVERKRFFRKSVEMT
EDDKVAESSPKDERIKAAMNIPRVDKLPSNVLRGGQEVKYEQCSKSTSEI
SKDCFKEKNEKEMEEEAEMKAVATSPSGRFLKFDIELGRGAFKTVYKGLD
TETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSWESI
LKGKKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHT
RTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFM
APEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGIK
PASFNKVTDPEVKEIIEGCIRQNKSERLSIRDLLNHAFFAEDTGLRVELA
EEDDCSNSSLALRLWVED*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4409
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term GST tag.; ; All WNK plasmids MUST be grown at 30 degrees C or less to prevent recombination.