cDNA Clone WNK3

• Plasmid: pEBG6P-1 WNK3 123-579
• Parent Plasmid: pCR2.1
• Expressed: GST-WNK3 123-579
• Genbank: NP_020922
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCACTAGTGGTACCGAGAAAAATGAAAAGGAAATGGAAGAA
GAAGCAGAAATGAAGGCTGTAGCTACTTCTCCTAGTGGCAGATTCCTGAA
ATTTGACATAGAACTAGGAAGAGGAGCATTTAAAACAGTATATAAAGGAC
TGGACACTGAAACATGGGTTGAGGTTGCTTGGTGTGAGCTGCAGGACCGA
AAGTTAACCAAAGCTGAGCAGCAAAGATTCAAGGAAGAAGCAGAGATGTT
GAAGGGTCTCCAGCACCCCAATATAGTTCGATTTTATGATTCCTGGGAAT
CTATATTAAAAGGAAAGAAATGTATTGTATTAGTGACTGAACTAATGACA
TCTGGGACCTTAAAGACGTACTTAAAACGATTTAAAGTCATGAAACCAAA
GGTCTTAAGGAGCTGGTGCAGGCAAATTTTAAAGGGGTTGCAGTTCTTGC
ACACTAGGACTCCTCCTATTATTCACCGGGATCTGAAGTGTGACAATATT
TTCATCACGGGACCCACTGGATCTGTGAAGATTGGTGATCTAGGATTAGC
CACCTTAATGCGTACCTCATTTGCTAAGAGTGTCATTGGAACTCCTGAGT
TTATGGCTCCAGAGATGTATGAAGAACACTATGATGAATCCGTAGATGTT
TATGCTTTTGGAATGTGTATGCTGGAAATGGCCACATCGGAGTATCCTTA
TTCTGAGTGTCAGAATGCAGCTCAAATATACCGGAAAGTAACTAGTGGCA
TAAAACCAGCCAGCTTCAATAAAGTCACTGATCCTGAAGTCAAAGAAATC
ATTGAAGGATGTATTCGTCAAAACAAATCTGAAAGGTTGTCTATCAGGGA
CCTATTAAACCATGCATTTTTTGCTGAGGATACAGGACTGAGGGTGGAGT
TAGCAGAAGAAGATGATTGCTCAAATTCATCCCTTGCTTTAAGACTCTGG
GTTGAAGACCCTAAAAAATTGAAAGGCAAACACAAAGACAATGAAGCTAT
TGAATTTAGTTTCAACTTAGAAACAGATACACCTGAGGAAGTAGCATATG
AAATGGTCAAGTCTGGGTTCTTCCATGAAAGTGATTCCAAAGCTGTTGCT
AAATCCATTAGAGACCGGGTGACGCCAATAAAGAAGACAAGAGAGAAGAA
GCCTGCTGGCTGTTTGGAAGAACGCAGGGATTCTCAGTGCAAGTCTATGG
GGAATGTATTCCCTCAGCCCCAGAATACAACTTTACCCCTTGCTCCCGCT
CAGCAAACTGGGGCTGAATGTGAAGAAACTGAAGTTGATCAACATGTTAG
ACAACAGCTTCTACAAAGAAAACCACAGCAGCACTGCTCCTCTGTTACAG
GTGACAATTTGTCTGAGGCAGGAGCTGCATCAGTTATACATTCATGAGCG
GCCGCG

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTEKNEKEMEE
EAEMKAVATSPSGRFLKFDIELGRGAFKTVYKGLDTETWVEVAWCELQDR
KLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSWESILKGKKCIVLVTELMT
SGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNI
FITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFMAPEMYEEHYDESVDV
YAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGIKPASFNKVTDPEVKEI
IEGCIRQNKSERLSIRDLLNHAFFAEDTGLRVELAEEDDCSNSSLALRLW
VEDPKKLKGKHKDNEAIEFSFNLETDTPEEVAYEMVKSGFFHESDSKAVA
KSIRDRVTPIKKTREKKPAGCLEERRDSQCKSMGNVFPQPQNTTLPLAPA
QQTGAECEETEVDQHVRQQLLQRKPQQHCSSVTGDNLSEAGAASVIHS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4617
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term GST.; ; All WNK plasmids MUST be grown at 30 degrees C or less to prevent recombination.