cDNA Clone WNK3

• Plasmid: pGEX6P-1-WNK3 1-579
• Parent Plasmid: pGEX6P1
• Expressed: GST-WNK3 1-579
• Genbank: NM_020922
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGGCCACTGATTCAGGGGATCCAGCCAGCACAGAAGATTCTGAGAAACCTGATGGAATTTCATTTGAAAACAGAGTTCCCCAGGTCGCTGCAACTTTGACAGTAGAAGCTAGACTAAAGGAGAAAAACAGTACCTTCTCTGCTTCTGGGGAAACTGTAGAAAGGAAGAGATTTTTCCGAAAGAGTGTTGAAATGACGGAAGATGACAAAGTTGCCGAATCATCCCCCAAAGATGAGAGAATTAAGGCCGCAATGAATATTCCAAGAGTAGATAAGCTTCCTTCAAATGTGTTGAGAGGTGGACAAGAAGTTAAATATGAACAGTGTTCAAAGTCAACCTCAGAAATCTCAAAAGATTGTTTCAAGGAGAAAAATGAAAAGGAAATGGAAGAAGAAGCAGAAATGAAGGCTGTAGCTACTTCTCCTAGTGGCAGATTCCTGAAATTTGACATAGAACTAGGAAGAGGAGCATTTAAAACAGTATATAAAGGACTGGACACTGAAACATGGGTTGAGGTTGCTTGGTGTGAGCTGCAGGACCGAAAGTTAACCAAAGCTGAGCAGCAAAGATTCAAGGAAGAAGCAGAGATGTTGAAGGGTCTCCAGCACCCCAATATAGTTCGATTTTATGATTCCTGGGAATCTATATTAAAAGGAAAGAAATGTATTGTATTAGTGACTGAACTAATGACATCTGGGACCTTAAAGACGTACTTAAAACGATTTAAAGTCATGAAACCAAAGGTCTTAAGGAGCTGGTGCAGGCAAATTTTAAAGGGGTTGCAGTTCTTGCACACTAGGACTCCTCCTATTATTCACCGGGACCTGAAGTGTGACAATATTTTCATCACGGGACCCACTGGATCTGTGAAGATTGGTGATCTAGGATTAGCCACCTTAATGCGTACCTCATTTGCTAAGAGTGTCATTGGAACTCCTGAGTTTATGGCTCCAGAGATGTATGAAGAACACTATGATGAATCCGTAGATGTTTATGCTTTTGGAATGTGTATGCTGGAAATGGCCACATCGGAGTATCCTTATTCTGAGTGTCAGAATGCAGCTCAAATATACCGGAAAGTAACTAGTGGCATAAAACCAGCCAGCTTCAATAAAGTCACTGATCCTGAAGTCAAGGAAATCATTGAAGGATGTATTCGTCAAAACAAATCTGAAAGGTTGTCTATCAGGGACCTATTAAACCATGCATTTTTTGCTGAGGATACAGGACTGAGGGTGGAGTTAGCAGAAGAAGATGATTGCTCAAATTCATCCCTTGCTTTAAGACTCTGGGTTGAAGACCCTAAAAAATTGAAAGGCAAACACAAAGACAATGAAGCTATTGAATTTAGTTTCAACCTAGAAACAGATACACCTGAGGAAGTAGCATATGAAATGGTCAAGTCTGGGTTCTTCCATGAAAGTGATTCCAAAGCTGTTGCTAAATCCATTAGAGACCGGGTGACGCCAATAAAGAAGACAAGAGAGAAGAAGCCTGCTGGCTGTTTGGAAGAACGCAGGGATTCTCAGTGCAAGTCTATGGGGAATGTATTCCCTCAGCCCCAGAATACAACTTTACCCCTTGCTCCCGCTCAGCAAACTGGGGCTGAATGTGAAGAAACTGAAGTTGATCAACATGTTAGACAACAGCTTCTACAAAGAAAACCACAGCAGCACTGCTCCTCTGTTACAGGTGACAATTTGTCTGAGGCAGGAGCTGCATCAGTTATACATTCATGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMATDSGDPASTEDSEK
PDGISFENRVPQVAATLTVEARLKEKNSTFSASGETVERKRFFRKSVEMT
EDDKVAESSPKDERIKAAMNIPRVDKLPSNVLRGGQEVKYEQCSKSTSEI
SKDCFKEKNEKEMEEEAEMKAVATSPSGRFLKFDIELGRGAFKTVYKGLD
TETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSWESI
LKGKKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHT
RTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFM
APEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGIK
PASFNKVTDPEVKEIIEGCIRQNKSERLSIRDLLNHAFFAEDTGLRVELA
EEDDCSNSSLALRLWVEDPKKLKGKHKDNEAIEFSFNLETDTPEEVAYEM
VKSGFFHESDSKAVAKSIRDRVTPIKKTREKKPAGCLEERRDSQCKSMGN
VFPQPQNTTLPLAPAQQTGAECEETEVDQHVRQQLLQRKPQQHCSSVTGD
NLSEAGAASVIHS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4631
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term GST.; ; All WNK plasmids MUST be grown at 30 degrees C or less to prevent recombination.