cDNA Clone WNK3

• Plasmid: pEBG6P-1 WNK3 1-579 (destroyed BamHI to allow BN cloning)
• Parent Plasmid: pEBG6P
• Expressed: GST-WNK3 1-579
• Genbank: NM_020922
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCATGGCCACTGATTCAGGCGATCCAGCCAGCACAGAAGAT
TCTGAGAAACCTGATGGAATTTCATTTGAAAACAGAGTTCCCCAGGTCGC
TGCAACTTTGACAGTAGAAGCTAGACTAAAGGAGAAAAACAGTACCTTCT
CTGCTTCTGGGGAAACTGTAGAAAGGAAGAGATTTTTCCGAAAGAGTGTT
GAAATGACGGAAGATGACAAAGTTGCCGAATCATCCCCCAAAGATGAGAG
AATTAAGGCCGCAATGAATATTCCAAGAGTAGATAAGCTTCCTTCAAATG
TGTTGAGAGGTGGACAAGAAGTTAAATATGAACAGTGTTCAAAGTCAACC
TCAGAAATCTCAAAAGATTGTTTCAAGGAGAAAAATGAAAAGGAAATGGA
AGAAGAAGCAGAAATGAAGGCTGTAGCTACTTCTCCTAGTGGCAGATTCC
TGAAATTTGACATAGAACTAGGAAGAGGAGCATTTAAAACAGTATATAAA
GGACTGGACACTGAAACATGGGTTGAGGTTGCTTGGTGTGAGCTGCAGGA
CCGAAAGTTAACCAAAGCTGAGCAGCAAAGATTCAAGGAAGAAGCAGAGA
TGTTGAAGGGTCTCCAGCACCCCAATATAGTTCGATTTTATGATTCCTGG
GAATCTATATTAAAAGGAAAGAAATGTATTGTATTAGTGACTGAACTAAT
GACATCTGGGACCTTAAAGACGTACTTAAAACGATTTAAAGTCATGAAAC
CAAAGGTCTTAAGGAGCTGGTGCAGGCAAATTTTAAAGGGGTTGCAGTTC
TTGCACACTAGGACTCCTCCTATTATTCACCGGGACCTGAAGTGTGACAA
TATTTTCATCACGGGACCCACTGGATCTGTGAAGATTGGTGATCTAGGAT
TAGCCACCTTAATGCGTACCTCATTTGCTAAGAGTGTCATTGGAACTCCT
GAGTTTATGGCTCCAGAGATGTATGAAGAACACTATGATGAATCCGTAGA
TGTTTATGCTTTTGGAATGTGTATGCTGGAAATGGCCACATCGGAGTATC
CTTATTCTGAGTGTCAGAATGCAGCTCAAATATACCGGAAAGTAACTAGT
GGCATAAAACCAGCCAGCTTCAATAAAGTCACTGATCCTGAAGTCAAGGA
AATCATTGAAGGATGTATTCGTCAAAACAAATCTGAAAGGTTGTCTATCA
GGGACCTATTAAACCATGCATTTTTTGCTGAGGATACAGGACTGAGGGTG
GAGTTAGCAGAAGAAGATGATTGCTCAAATTCATCCCTTGCTTTAAGACT
CTGGGTTGAAGACCCTAAAAAATTGAAAGGCAAACACAAAGACAATGAAG
CTATTGAATTTAGTTTCAACCTAGAAACAGATACACCTGAGGAAGTAGCA
TATGAAATGGTCAAGTCTGGGTTCTTCCATGAAAGTGATTCCAAAGCTGT
TGCTAAATCCATTAGAGACCGGGTGACGCCAATAAAGAAGACAAGAGAGA
AGAAGCCTGCTGGCTGTTTGGAAGAACGCAGGGATTCTCAGTGCAAGTCT
ATGGGGAATGTATTCCCTCAGCCCCAGAATACAACTTTACCCCTTGCTCC
CGCTCAGCAAACTGGGGCTGAATGTGAAGAAACTGAAGTTGATCAACATG
TTAGACAACAGCTTCTACAAAGAAAACCACAGCAGCACTGCTCCTCTGTT
ACAGGTGACAATTTGTCTGAGGCAGGAGCTGCATCAGTTATACATTCATG
AGCGGCCGCG

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMATDSGDPASTED
SEKPDGISFENRVPQVAATLTVEARLKEKNSTFSASGETVERKRFFRKSV
EMTEDDKVAESSPKDERIKAAMNIPRVDKLPSNVLRGGQEVKYEQCSKST
SEISKDCFKEKNEKEMEEEAEMKAVATSPSGRFLKFDIELGRGAFKTVYK
GLDTETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSW
ESILKGKKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQF
LHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLMRTSFAKSVIGTP
EFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTS
GIKPASFNKVTDPEVKEIIEGCIRQNKSERLSIRDLLNHAFFAEDTGLRV
ELAEEDDCSNSSLALRLWVEDPKKLKGKHKDNEAIEFSFNLETDTPEEVA
YEMVKSGFFHESDSKAVAKSIRDRVTPIKKTREKKPAGCLEERRDSQCKS
MGNVFPQPQNTTLPLAPAQQTGAECEETEVDQHVRQQLLQRKPQQHCSSV
TGDNLSEAGAASVIHS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4633
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term GST.; ; All WNK plasmids MUST be grown at 30 degrees C or less to prevent recombination.