cDNA Clone UBE2E3

• Plasmid: pET28a(+) UBE2E3
• Parent Plasmid: pET28a original
• Expressed: 6H-UBE2E3
• Genbank: NM_006357
• Tag: 6His
• Species: Human

Sequence of Insert

AGATCTATGTCCAGTGATAGGCAAAGGTCCGATGATGAGAGCCCCAGCAC
CAGCAGTGGCAGTTCAGATGCGGACCAGCGAGACCCAGCCGCTCCAGAGC
CTGAAGAACAAGAGGAAAGAAAACCTTCTGCCACCCAGCAGAAGAAAAAC
ACCAAACTCTCTAGCAAAACCACTGCTAAGTTATCCACTAGTGCTAAAAG
AATTCAGAAGGAGCTAGCTGAAATAACCCTTGATCCTCCTCCTAATTGCA
GTGCTGGGCCTAAAGGAGATAACATTTATGAATGGAGATCAACTATACTT
GGTCCACCGGGTTCTGTATATGAAGGTGGTGTGTTTTTTCTGGATATCAC
ATTTTCATCAGATTATCCATTTAAGCCACCAAAGGTTACTTTCCGCACCA
GAATCTATCACTGCAACATCAACAGTCAGGGAGTCATCTGTCTGGACATC
CTTAAAGACAACTGGAGTCCCGCTTTGACTATTTCAAAGGTTTTGCTGTC
TATTTGTTCCCTTTTGACAGACTGCAACCCTGCGGATCCTCTGGTTGGAA
GCATAGCCACTCAGTATTTGACCAACAGAGCAGAACACGACAGGATAGCC
AGACAGTGGACCAAGAGATACGCAACATAAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRRSMSSDRQRSDDESPSTS
SGSSDADQRDPAAPEPEEQEERKPSATQQKKNTKLSSKTTAKLSTSAKRI
QKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITF
SSDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSI
CSLLTDCNPADPLVGSIATQYLTNRAEHDRIARQWTKRYAT*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU14049
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for bacterial expression.; ; Estimated MW of expressed protein : 26554 Daltons