cDNA Clone UBE2J1

• Plasmid: pGEX6P-1-UBE2J1
• Parent Plasmid: pGEX6P1
• Expressed: GST-UBE2J1
• Genbank: NM_016021
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGAGACCCGCTACAACCTGAAGAGTCCGGCTGTTAAACGTTT
AATGAAAGAAGCGGCAGAATTGAAAGATCCAACAGATCATTACCATGCGC
AGCCTTTAGAGGATAACCTTTTTGAATGGCACTTCACGGTTAGAGGGCCC
CCAGACTCCGATTTTGATGGAGGAGTTTATCACGGGCGGATAGTACTGCC
ACCAGAGTATCCCATGAAACCACCAAGCATTATTCTCCTAACGGCTAATG
GTCGATTTGAAGTGGGCAAGAAAATCTGTTTGAGCATCTCAGGCCATCAT
CCTGAAACTTGGCAGCCTTCGTGGAGTATAAGGACAGCATTATTAGCCAT
CATTGGGTTTATGCCAACAAAAGGAGAGGGAGCCATAGGTTCTCTAGATT
ACACTCCTGAGGAAAGAAGAGCACTTGCCAAAAAATCACAAGATTTCTGT
TGTGAAGGATGTGGCTCTGCCATGAAGGATGTCCTGTTGCCTTTAAAATC
TGGAAGCGATTCAAGCCAAGCTGACCAAGAAGCCAAAGAACTGGCTAGGC
AAATAAGCTTTAAGGCAGAAGTCAATTCATCTGGAAAGACTATCTCTGAG
TCAGACTTAAACCACTCTTTTTCACTAACTGATTTACAAGATGATATACC
TACAACATTCCAGGGTGCTACGGCCAGTACATCGTACGGACTCCAGAATT
CCTCAGCAGCATCCTTTCATCAACCTACCCAACCTGTAGCTAAGAATACC
TCCATGAGCCCTCGACAGCGCCGGGCCCAGCAGCAGAGTCAGAGAAGGTT
GTCTACTTCACCAGATGTAATCCAGGGCCACCAGCCAAGAGACAACCACA
CTGATCATGGTGGGTCAGCTGTACTGATTGTCATCCTGACTTTGGCATTG
GCAGCTCTTATATTCCGACGAATATATCTGGCAAACGAATACATATTTGA
CTTTGAGTTATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMETRYNLKSPAVKRLMKEA
AELKDPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHGRIVLPPEYP
MKPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAIIGFM
PTKGEGAIGSLDYTPEERRALAKKSQDFCCEGCGSAMKDVLLPLKSGSDS
SQADQEAKELARQISFKAEVNSSGKTISESDLNHSFSLTDLQDDIPTTFQ
GATASTSYGLQNSSAASFHQPTQPVAKNTSMSPRQRRAQQQSQRRLSTSP
DVIQGHQPRDNHTDHGGSAVLIVILTLALAALIFRRIYLANEYIFDFEL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU14057
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 62020 Daltons