cDNA Clone AS160

• Synonyms: TBC1D4
• Plasmid: pGFP.TAP AS160A
• Parent Plasmid: pEGFP TAP
• Expressed: GFP-TAP-AS160A
• Tag: GFP
• Tag: TAP
• Species: Human

Sequence of Insert

ggatccATGGAGCCGCCCAGCTGCATTCAGGATGAGCCGTTCCCGCACCC
CCTGGAGCCCGAGCCGGGCGTCTCAGCTCAGCCCGGCCCCGGGAAGCCAA
GCGATAAGCGGTTCCGGCTGTGGTACGTTGGGGGGTCGTGCCTGGACCAC
AGGACCACGCTGCCTATGCTGCCCTGGCTCATGGCCGAGATCCGCAGGCG
CAGCCAGAAGCCCGAGGCGGGCGGCTGCGGGGCGCCGGCGGCCCGAGAGG
TGATCCTGGTGCTCAGCGCGCCCTTCCTGCGTTGCGTCCCCGCGCCGGGC
GCTGGGGCCTCGGGGGGCACTAGTCCGTCGGCCACGCAGCCCAACCCGGC
GGTATTCATCTTCGAGCACAAGGCGCAGCATATCTCGCGCTTCATCCACA
ACAGCCACGACCTCACCTACTTTGCCTACCTGATCAAGGCGCAGCCCGAC
GACCCCGAGTCGCAGATGGCCTGCCACGTTTTCCGCGCCACAGACCCCAG
CCAGGTTCCTGATGTTATTAGCAGCATAAGGCAATTATCTAAAGCGGCCA
TGAAAGAGGATGCCAAACCCAGCAAAGATAATGAGGACGCCTTTTACAAC
TCTCAGAAGTTCGAAGTCCTGTACTGTGGAAAGGTGACCGTGACCCACAA
GAAGGCCCCCTCAAGCCTCATCGATGACTGCATGGAGAAGTTCAGCCTGC
ACGAACAGCAGCGCCTGAAGATCCAAGGCGAGCAGCGCGGTCCGGACCCA
GGAGAGGACCTGGCTGACTTGGAGGTGGTGGTGCCCGGGTCCCCCGGAGA
CTGCCTGCCGGAGGAGGCTGACGGCACCGACACCCACCTTGGCTTACCTG
CCGGGGCCAGCCAGCCTGCCCTGACCAGCTCTCGGGTCTGCTTCCCTGAG
CGGATTTTGGAAGATTCTGGCTTTGATGAGCAGCAGGAGTTTCGGTCTCG
GTGCAGCAGTGTCACCGGCGTGCAACGGAGAGTTCACGAGGGCAGCCAGA
AATCCCAGCCGCGACGGAGACACGCGAGCGCACCCAGTCACGTCCAGCCC
TCGGACTCGGAGAAGAACAGGACCATGCTCTTCCAGGTTGGGCGATTTGA
GATTAACCTTATCAGTCCAGACACTAAATCAGTTGTGCTAGAAAAGAATT
TTAAAGATATCTCCCCTTGTTCTCAGGGTATAAAGCATGTGGATCACTTT
GGCTTTATCTGCCGGGAGTCTCCAGAGCCTGGACTTAGCCAGTATATTTG
TTATGTATTCCAGTGTGCCAGCGAATCTCTGGTTGATGAGGTAATGCTGA
CTCTGAAACAGGCCTTCAGTACGGCGGCTGCCCTGCAGAGTGCCAAGACG
CAGATTAAACTGTGTGAGGCCTGCCCGATGCACTCTTTGCATAAGCTCTG
TGAAAGGATTGAAGGTCTCTACCCACCAAGAGCCAAGCTGGTGATACAGA
GGCATCTCTCATCACTGACAGATAATGAGCAAGCTGACATCTTTGAAAGA
GTTCAGAAAATGAAGCCAGTCAGTGACCAGGAAGAAAATGAACTTGTGAT
TTTACACCTGAGGCAGCTGTGTGAAGCCAAGCAGAAGACACACGTGCACA
TCGGGGAAGGCCCTGCTACTATTTCAAATAGTACAATCCCAGAAAATGCA
ACAAGCAGTGGAAGGTTCAAACTTGACATTCTGAAAAATAAAGCTAAGAG
ATCCTTAACTAGCTCCCTGGAAAATATCTTCTCAAGGGGAGCTAACAGAA
TGAGAGGTCGGCTTGGAAGTGTGGACAGTTTTGAACGGTCCAACAGTCTT
GCTTCAGAGAAGGACTACTCACCAGGGGATTCTCCACCAGGGACACCGCC
AGCGTCCCCACCGTCCTCAGCTTGGCAAACGTTTCCCGAAGAGGATTCCG
ACTCCCCGCAGTTTCGAAGACGGGCACACACGTTCAGCCACCCACCTTCA
AGCACAAAGAGAAAGCTGAATTTGCAGGATGGGAGGGCTCAGGGTGTGCG
TTCCCCTCTGCTGAGGCAGAGCTCCAGTGAACAGTGCAGTGATGGAGAAG
GGAGAAAAAGGACCTCATCTACCTGCAGCAATGAGTCCCTAAGTGTGGGA
GGAACCTCTGTCACTCCTCGCCGGATCTCCTGGCGGCAGCGCATTTTCCT
CAGGGTTGCTTCTCCCATGAACAAATCTCCCTCAGCAATGCAACAGCAAG
ATGGATTGGACAGGAACGAGCTGCTGCCACTGTCCCCCCTCTCTCCAACC
ATGGAGGAGGAACCGCTGGTTATATTCCTGTCTGGGGAGGATGACCCAGA
AAAGATTGAAGAAAGAAAGAAATCAAAAGAACTGAGGAGCTTGTGGAGAA
AAGCTATACACCAACAAATCTTGTTACTTCGAATGGAAAAAGAAAACCAG
AAACTTGAAGCAAGCAGAGATGAACTCCAGTCCAGAAAAGTTAAATTAGA
CTATGAAGAAGTTGGTGCATGTCAGAAAGAGGTCTTAATAACTTGGGATA
AGAAGTTGTTAAACTGCAGAGCTAAAATCAGATGTGATATGGAAGATATT
CATACTCTTCTTAAAGAAGGAGTTCCCAAAAGTCGACGAGGAGAAATTTG
GCAGTTTCTGGCTTTACAGTACCGACTCAGACACAGATTGCCTAATAAAC
AACAGCCTCCTGACATATCCTATAAGGAACTTTTGAGGCAGCTCACTGCT
CAGCAGCATGCGATTCTTGTGGATTTAGGAAGGACGTTTCCTACTCACCC
TTACTTTTCAGTACAGCTTGGGCCAGGACAGCTGTCACTGTTTAACCTCC
TGAAAGCCTATTCTTTGCTGGACAAAGAAGTGGGATACTGTCAGGGGATC
AGCTTTGTGGCTGGAGTCCTGCTTCTGCACATGAGTGAAGAGCAAGCCTT
TGAAATGCTGAAATTCCTCATGTATGACCTCGGCTTCCGCAAGCAGTACA
GACCTGACATGATGTCGCTGCAGATTCAAATGTACCAGCTGTCCAGGCTC
CTTCATGACTATCACAGAGATCTCTACAATCACCTTGAAGAAAATGAAAT
CAGCCCCAGTCTTTATGCTGCCCCCTGGTTCCTCACATTGTTTGCCTCTC
AGTTTTCATTAGGATTTGTAGCCAGAGTTTTTGATATTATTTTTCTTCAG
GGAACTGAAGTTATATTCAAGGTTGCACTCAGCCTACTGAGCAGCCAAGA
GACACTTATAATGGAATGTGAGAGCTTTGAAAATATTGTTGAGTTTCTTA
AAAACACGCTACCTGATATGAATACCTCTGAAATGGAAAAAATTATTACC
CAGGTTTTTGAGATGGATATTTCTAAGCAGTTGCATGCCTATGAGGTGGA
ATATCATGTGCTACAGGATGAGCTTCAGGAATCTTCATATTCCTGTGAGG
ATAGTGAAACTTTGGAGAAGCTGGAGAGGGCCAATAGCCAACTGAAAAGA
CAAAACATGGACCTCCTAGAAAAATTACAGGTAGCTCATACTAAAATCCA
GGCCTTGGAATCAAACCTGGAAAATCTTTTGACGAGAGAGACCAAAATGA
AGTCTTTAATCCGGACCCTGGAACAAGAAAAAATGGCTTATCAAAAGACA
GTGGAGCAACTCCGGAAGCTGCTGCCCGCGGATGCTCTAGCCAATTGTGA
CCTGTTGCTGAGAGACCTAAACTGCAACCCTAACAACAAAGCCAAGATAG
GAAATAAGCCATAAggatcc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
DIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK
DDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQR
NAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGR
SMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPLGSMEP
PSCIQDEPFPHPLEPEPGVSAQPGPGKPSDKRFRLWYVGGSCLDHRTTLP
MLPWLMAEIRRRSQKPEAGGCGAPAAREVILVLSAPFLRCVPAPGAGASG
GTSPSATQPNPAVFIFEHKAQHISRFIHNSHDLTYFAYLIKAQPDDPESQ
MACHVFRATDPSQVPDVISSIRQLSKAAMKEDAKPSKDNEDAFYNSQKFE
VLYCGKVTVTHKKAPSSLIDDCMEKFSLHEQQRLKIQGEQRGPDPGEDLA
DLEVVVPGSPGDCLPEEADGTDTHLGLPAGASQPALTSSRVCFPERILED
SGFDEQQEFRSRCSSVTGVQRRVHEGSQKSQPRRRHASAPSHVQPSDSEK
NRTMLFQVGRFEINLISPDTKSVVLEKNFKDISPCSQGIKHVDHFGFICR
ESPEPGLSQYICYVFQCASESLVDEVMLTLKQAFSTAAALQSAKTQIKLC
EACPMHSLHKLCERIEGLYPPRAKLVIQRHLSSLTDNEQADIFERVQKMK
PVSDQEENELVILHLRQLCEAKQKTHVHIGEGPATISNSTIPENATSSGR
FKLDILKNKAKRSLTSSLENIFSRGANRMRGRLGSVDSFERSNSLASEKD
YSPGDSPPGTPPASPPSSAWQTFPEEDSDSPQFRRRAHTFSHPPSSTKRK
LNLQDGRAQGVRSPLLRQSSSEQCSDGEGRKRTSSTCSNESLSVGGTSVT
PRRISWRQRIFLRVASPMNKSPSAMQQQDGLDRNELLPLSPLSPTMEEEP
LVIFLSGEDDPEKIEERKKSKELRSLWRKAIHQQILLLRMEKENQKLEAS
RDELQSRKVKLDYEEVGACQKEVLITWDKKLLNCRAKIRCDMEDIHTLLK
EGVPKSRRGEIWQFLALQYRLRHRLPNKQQPPDISYKELLRQLTAQQHAI
LVDLGRTFPTHPYFSVQLGPGQLSLFNLLKAYSLLDKEVGYCQGISFVAG
VLLLHMSEEQAFEMLKFLMYDLGFRKQYRPDMMSLQIQMYQLSRLLHDYH
RDLYNHLEENEISPSLYAAPWFLTLFASQFSLGFVARVFDIIFLQGTEVI
FKVALSLLSSQETLIMECESFENIVEFLKNTLPDMNTSEMEKIITQVFEM
DISKQLHAYEVEYHVLQDELQESSYSCEDSETLEKLERANSQLKRQNMDL
LEKLQVAHTKIQALESNLENLLTRETKMKSLIRTLEQEKMAYQKTVEQLR
KLLPADALANCDLLLRDLNCNPNNKAKIGNKP

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU7059
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Protein sequence has the following mutations compared to NM_014832 : ; M1K, S387P and K958R.; The cloned sequence represents a splice variant lacking amino acids 679-741. The majority of ESTs in the NCBI database represent this form.; A further single amino acid deletion (G865) is also documented in the NCBI EST database.