cDNA Clone Bul1

• Plasmid: pCMV5-FLAG-1 Bul1
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG-1 Bul1
• Tag: FLAG
• Species: Human

Sequence of Insert

ATGCTGCTGCACCTGTGTAGTGTGAAGAATCTGTACCAGAACAGGTTTTT
AGGCCTGGCCGCCATGGCGTCTCCTTCTAGAAACTCCCAGAGCCGACGCC
GGTGCAAGGAGCCGCTCCGATACAGCTACAACCCCGACCAGTTCCACAAC
ATGGACCTCAGGGGCGGCCCCCACGATGGCGTCACCATTCCCCGCTCCAC
CAGCGACACTGACCTGGTCACCTCGGACAGCCGCTCCACGCTCATGGTCA
GCAGCTCCTACTATTCCATCGGGCACTCTCAGGACCTGGTCATCCACTGG
GACATAAAGGAGGAAGTGGACGCTGGGGACTGGATTGGCATGTACCTCAT
TGATGAGGTCTTGTCCGAAAACTTTCTGGACTATAAAAACCGTGGAGTCA
ATGGTTCTCATCGGGGCCAGATCATCTGGAAGATCGATGCCAGCTCGTAC
TTTGTGGAACCTGAAACTAAGATCTGCTTCAAATACTACCATGGAGTGAG
TGGGGCCCTGCGAGCAACCACCCCCAGTGTCACGGTCAAAAACTCGGCAG
CTCCTATTTTTAAAAGCATTGGTGCTGATGAGACCGTCCAAGGACAAGGA
AGTCGGAGGCTGATCAGCTTCTCTCTCTCAGATTTCCAAGCCATGGGGTT
GAAGAAAGGGATGTTTTTCAACCCAGACCCTTATCTGAAGATTTCCATTC
AGCCTGGGAAACACAGCATCTTCCCCGCCCTCCCTCACCATGGACAGGAG
AGGAGATCCAAGATCATAGGCAACACCGTGAACCCCATCTGGCAGGCCGA
GCAATTCAGTTTTGTGTCCTTGCCCACTGACGTGCTGGAAATTGAGGTGA
AGGACAAGTTTGCCAAGAGCCGCCCCATCATCAAGCGCTTCTTGGGAAAG
CTGTCGATGCCCGTTCAAAGACTCCTGGAGAGACACGCCATAGGGGATAG
GGTGGTCAGCTACACACTTGGCCGCAGGCTTCCAACAGATCATGTGAGTG
GACAGCTGCAATTCCGATTTGAGATCACTTCCTCCATCCACCCAGATGAT
GAGGAGATTTCCCTGAGTACCGAGCCTGAGTCAGCCCAAATTCAGGACAG
CCCCATGAACAACCTGATGGAAAGCGGCAGTGGGGAACCTCGGTCTGAGG
CACCAGAGTCCTCTGAGAGCTGGAAGCCAGAGCAGCTGGGTGAGGGCAGT
GTCCCCGATGGTCCAGGGAACCAAAGCATAGAGCTTTCCAGACCAGCTGA
GGAAGCAGCAGTCATCACGGAGGCAGGAGACCAGGGCATGGTCTCTGTGG
GACCTGAAGGGGCTGGGGAGCTCCTGGCCCAGGTGCAAAAGGACATCCAG
CCTGCCCCCAGTGCAGAAGAGCTGGCCGAGCAGCTGGACCTGGGTGAGGA
GGCATCAGCACTGCTGCTGGAAGACGGTGAAGCCCCAGCCAGCACCAAGG
AGGAGCCCTTGGAGGAGGAAGCAACGACCCAGAGCCGGGCTGGAAGGGAA
GAAGAGGAGAAGGAGCAGGAGGAGGAGGGAGATGTGTCTACCCTGGAGCA
GGGAGAGGGCAGGCTGCAGCTGCGGGCCTCGGTGAAGAGAAAAAGCAGGC
CCTGCTCCTTGCCTGTGTCCGAGCTGGAGACGGTGATCGCGTCAGCCTGC
GGGGACCCCGAGACCCCGCGGACACACTACATCCGCATCCACACCCTGCT
GCACAGCATGCCCTCCGCCCAGGGCGGCAGCGCGGCAGAGGAGGAGGACG
GCGCGGAGGAGGAGTCCACCCTCAAGGACTCCTCGGAGAAGGATGGGCTC
AGCGAGGTGGACACGGTGGCCGCTGACCCGTCTGCCCTGGAAGAGGACAG
AGAAGAGCCCGAGGGGGCTACTCCAGGCACGGCGCACCCTGGCCACTCCG
GGGGCCACTTCCCCAGCCTGGCCAATGGCGCGGCCCAGGATGGCGACACG
CACCCCAGCACCGGGAGCGAGAGCGACTCCAGCCCCAGGCAAGGCGGGGA
CCACAGTTGCGAGGGCTGTGACGCGTCCTGCTGCAGCCCCTCGTGCTACA
GCTCCTCGTGCTACAGCACGTCCTGCTACAGCAGCTCGTGCTACAGCGCC
TCGTGCTACAGCCCCTCCTGCTACAACGGCAACAGGTTCGCCAGCCACAC
GCGCTTCTCCTCCGTGGACAGCGCCAAGATCTCCGAGAGCACGGTCTTCT
CCTCGCAAGACGACGAGGAGGAGGAGAACAGCGCGTTCGAGTCGGTACCC
GACTCCATGCAGAGCCCTGAGCTGGACCCGGAGTCCACGAACGGCGCTGG
GCCGTGGCAAGACGAGCTGGCCGCCCCTAGCGGGCACGTGGAAAGAAGCC
CGGAAGGTCTGGAATCCCCCGTGGCAGGTCCAAGCAATCGGAGAGAAGGT
GAATGTCCTATACTCCATAATTCCCAGCCAGTAAGCCAGCTTCCTTCCCT
GAGGCCTGAACATCATCACTACCCAACAATCGATGAGCCTCTTCCACCAA
ACTGGGAAGCTCGAATTGACAGCCACGGGCGGGTCTTTTATGTGGACCAC
GTGAACCGCACAACCACCTGGCAGCGTCCGACGGCAGCAGCCACCCCGGA
TGGCATGCGGAGATCGGGGTCCATCCAGCAGATGGAGCAACTCAACAGGC
GGTATCAAAACATTCAGCGAACCATTGCAACAGAGAGGTCCGAAGAAGAT
TCTGGCAGCCAAAGCTGCGAGCAAGCCCCAGCAGGAGGAGGCGGAGGTGG
AGGGAGTGACTCAGAAGCCGAATCTTCCCAGTCCAGCTTAGATCTAAGGA
GAGAGGGGTCACTTTCTCCAGTGAACTCACAAAAAATCACCTTGCTGCTG
CAGTCCCCAGCGGTCAAGTTCATCACCAACCCCGAGTTCTTCACTGTGCT
ACATGCCAATTATAGTGCCTACCGAGTCTTCACCAGTAGCACCTGCTTAA
AGCACATGATTCTGAAAGTCCGACGGGATGCTCGCAATTTTGAACGCTAC
CAGCACAACCGGGACTTGGTGAATTTCATCAACATGTTCGCAGACACTCG
GCTGGAACTGCCCCGGGGCTGGGAGATCAAAACGGACCAGCAGGGAAAGT
CTTTTTTCGTGGACCACAACAGTCGAGCTACCACTTTCATTGACCCCCGA
ATCCCTCTTCAGAACGGTCGTCTTCCCAATCATCTAACTCACCGACAGCA
CCTCCAGAGGCTCCGAAGTTACAGCGCTGGAGAGGCCTCAGAAGTTTCTA
GAAACAGAGGAGCCTCTTTACTGGCCAGGCCAGGACACAGCTTAGTAGCT
GCTATTCGAAGCCAACATCAACATGAGTCATTGCCACTGGCATATAATGA
CAAGATTGTGGCATTTCTTCGCCAGCCAAACATTTTTGAAATGCTGCAAG
AGCGTCAGCCAAGCTTAGCAAGAAACCACACACTCAGGGAGAAAATCCAT
TACATTCGGACTGAGGGTAATCACGGGCTTGAGAAGTTGTCCTGTGATGC
GGATCTGGTCATTTTGCTGAGTCTCTTTGAAGAAGAGATTATGTCCTACG
TCCCCCTGCAGGCTGCCTTCCACCCTGGGTATAGCTTCTCTCCCCGATGT
TCACCCTGTTCTTCACCTCAGAACTCCCCAGGTTTACAGAGAGCCAGTGC
AAGAGCCCCTTCCCCCTACCGAAGAGACTTTGAGGCCAAGCTCCGCAATT
TCTACAGAAAACTGGAAGCCAAAGGATTTGGTCAGGGTCCGGGGAAAATT
AAGCTCATTATTCGCCGGGATCATTTGTTGGAGGGAACCTTCAATCAGGT
GATGGCCTATTCGCGGAAAGAGCTCCAGCGAAACAAGCTCTACGTCACCT
TTGTTGGAGAGGAGGGCCTGGACTACAGTGGCCCCTCGCGGGAGTTCTTC
TTCCTTCTGTCTCAGGAGCTCTTCAACCCTTACTATGGACTCTTTGAGTA
CTCGGCAAATGATACTTACACGGTGCAGATCAGCCCCATGTCCGCATTTG
TAGAAAACCATCTTGAGTGGTTCAGGTTTAGCGGTCGCATCCTGGGTCTG
GCTCTGATCCATCAGTACCTTCTTGACGCTTTCTTCACGAGGCCCTTCTA
CAAGGCACTCCTGAGACTGCCCTGTGATTTGAGTGACCTGGAATATTTGG
ATGAGGAATTCCACCAGAGTTTGCAGTGGATGAAGGACAACAACATCACA
GACATCTTAGACCTCACTTTCACTGTTAATGAAGAGGTTTTTGGACAGGT
CACGGAAAGGGAGTTGAAGTCTGGAGGAGCCAACACACAGGTGACGGAGA
AAAACAAGAAGGAGTACATCGAGCGCATGGTGAAGTGGCGGGTGGAGCGC
GGCGTGGTACAGCAGACCGAGGCGCTGGTGCGCGGCTTCTACGAGGTTGT
AGACTCGAGGCTGGTGTCCGTGTTTGATGCCAGGGAGCTGGAGCTGGTGA
TAGCTGGCACCGCGGAAATCGACCTAAATGACTGGCGGAATAACACTGAG
TACCGGGGAGGTTACCACGATGGGCATCTTGTGATCCGCTGGTTCTGGGC
TGCGGTGGAGCGCTTCAATAATGAGCAGAGGCTGAGATTACTGCAGTTTG
TCACGGGAACATCCAGCGTGCCCTACGAAGGCTTCGCAGCCCTCCGTGGG
AGCAATGGGCTTCGGCGCTTCTGCATAGAGAAATGGGGGAAAATTACTTC
TCTCCCCAGGGCACACACATGCTTCAACCGACTGGATCTTCCACCGTATC
CCTCGTACTCCATGTTGTATGAAAAGCTGTTAACAGCAGTAGAGGAAACC
AGCACCTTTGGACTTGAGTGA

Amino Acid Sequence

DYKDDDDKGSMLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSIELSRPAEEAAVITEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20221
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: g1210c (confers Gly404Arg) - this appears in other sequences / ESTs on Unigene; t1539c (silent mutation, Ser513); a3597c (silent mutation); ; Estimated MW of expressed protein : 180694.82 Daltons