cDNA Clone USP22

• Plasmid: pGEX6P-1-USP22
• Parent Plasmid: pGEX6P1
• Expressed: GST USP22
• Genbank: NM_015276.1 GI:150010638
• Tag: GST
• Species: Human

Sequence of Insert

ggatccatggtgtcccggccagagcccgagggcgaggccatggacgccga
gctggcggtagcgccgccgggctgctcgcacctgggcagcttcaaggtgg
acaactggaagcagaacctgcgggccatctaccagtgcttcgtgtggagc
ggcacggctgaggcccgcaagcgcaaggccaagtcctgtatctgccatgt
ctgtggcgtccacctcaacaggctgcattcctgcctctactgtgtcttct
tcggctgtttcacaaagaagcatattcacgagcatgcgaaggcgaagcgg
cacaacctggccattgatctgatgtacggaggcatctactgttttctgtg
ccaggactacatctatgacaaagacatggaaataatcgccaaggaggagc
agcgaaaagcttggaaaatgcaaggcgttggagagaagttttcaacttgg
gaaccaaccaaacgggagcttgaactgctgaagcacaacccgaaaaggag
aaagatcacctcgaactgcaccataggtctgcgtgggctgatcaaccttg
ggaacacatgcttcatgaactgcatcgtgcaggccctgacccacacgcca
cttctgcgggacttcttcctgtctgacaggcaccgctgtgagatgcagag
ccccagctcctgtctggtctgtgagatgtcctcactgtttcaggagtttt
actctggacaccggtcccctcacatcccgtataagttgctgcacctggtg
tggacccacgcgaggcacctagcaggctacgagcagcaggacgcccacga
gttcctcatcgcggccctggacgtgctccaccgacactgcaaaggtgatg
acaatgggaagaaggccaacaaccccaaccactgcaactgcatcatagac
cagatcttcacaggcgggttgcagtcagacgtcacctgccaagtctgcca
tggagtctccaccaccatcgaccccttctgggacatcagcttggatctcc
ccggctcttccaccccattctggcccctgagcccagggagcgagggcaac
gtggtaaacggggaaagccacgtgtcgggaaccaccacgctcacggactg
cctgcgacgattcaccagaccagagcacttgggcagcagcgccaagatca
agtgcagcggttgccatagctaccaggagtccacaaagcagctcactatg
aagaaactgcccatcgtagcctgttttcatctcaaacgatttgaacactc
agccaagctgcggcggaagatcaccacgtatgtgtccttccccctggagc
tggacatgacccctttcatggcctccagcaaagagagcaggatgaatgga
cagtaccagcagcccacggacagtctcaacaatgacaacaagtattccct
gtttgctgttgttaaccatcaagggaccttggagagtggccactacacca
gctttatccggcagcacaaagaccagtggttcaagtgtgacgatgccatc
atcaccaaggccagcatcaaggacgtcctggacagcgaagggtacttgct
gttctatcacaaacagttcctggaatacgagtaggtcgac

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRGSMVSRPEPEGEAMD
AELAVAPPGCSHLGSFKVDNWKQNLRAIYQCFVWSGTAEARKRKAKSCIC
HVCGVHLNRLHSCLYCVFFGCFTKKHIHEHAKAKRHNLAIDLMYGGIYCF
LCQDYIYDKDMEIIAKEEQRKAWKMQGVGEKFSTWEPTKRELELLKHNPK
RRKITSNCTIGLRGLINLGNTCFMNCIVQALTHTPLLRDFFLSDRHRCEM
QSPSSCLVCEMSSLFQEFYSGHRSPHIPYKLLHLVWTHARHLAGYEQQDA
HEFLIAALDVLHRHCKGDDNGKKANNPNHCNCIIDQIFTGGLQSDVTCQV
CHGVSTTIDPFWDISLDLPGSSTPFWPLSPGSEGNVVNGESHVSGTTTLT
DCLRRFTRPEHLGSSAKIKCSGCHSYQESTKQLTMKKLPIVACFHLKRFE
HSAKLRRKITTYVSFPLELDMTPFMASSKESRMNGQYQQPTDSLNNDNKY
SLFAVVNHQGTLESGHYTSFIRQHKDQWFKCDDAIITKASIKDVLDSEGY
LLFYHKQFLEYE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU21096
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated Mw of expressed protein : 87381 Daltons