cDNA Clone UBXD7

• Plasmid: pGEX-TEV-FLAG UBXD7 del. UAS Domain No ATG
• Parent Plasmid: pGEX TEV TEV
• Expressed: GST-FLAG UBXD7 Del. UAS Domain
• Genbank: NM_015562
• Tag: GST
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCGCTGCCCACGGGGGCTCCGCGGCGTCCTCGGCGCTGAAGGGGTT
AATTCAACAGTTCACCACCATTACCGGTGCAAGTGAAAGTGTAGGAAAAC
ATATGCTTGAAGCGTGCAACAATAATCTGGAAATGGCAGTCACTATGTTT
TTGGATGGTGGAGGAATCGCTGAAGAGCCCAGTACCAGTTCAGCAAGTGT
CTCTACTGTCAGACCACACACAGAAGAAGAAGTTCGTGCCCCAATTCCTC
AAAAGCAGGAAATACTGGTGGAACCAGAACCATTATTTGGTGCTCCTAAA
AGACGACGGCCTGCACGTTCAATTTTTGATGGTTTCCGGGATTTTCAGAC
TGAAACTATTCGGCAAGAACAAGAATTAAGAAATGGAGGAGCTATCGATA
AGAAATTAACTCATGGACAACTGGATGGACTTTCTAGCAGTCCCCCCAAA
AAATGTGCCCGTTCCGAGAGCCTTATAGATGCAAGTGAAGACAGCCAGCT
AGAAGCTGCCATCAGAGCCTCCTTACAAGAAACACATTTTGATTCAACAC
AGACAAAACAGGATAGCCGCTCAGATGAAGAATCTGAATCTGAACTTTTT
TCTGGCAGTGAGGAGTTCATATCCGTTTGTGGCTCTGATGAAGAAGAAGA
GGTAGAGAATCTTGCCAAGTCCAGAAAGTCTCCCCACAAAGATTTGGGGC
ATAGAAAAGAGGAGAATAGAAGGCCGCTGACTGAGCCACCAGTCAGAACT
GATCCTGGAACAGCCACAAACCACCAAGGATTGCCAGCTGTGGATTCAGA
GATACTGGAGATGCCACCTGAAAAAGCAGATGGAGTAGTGGAGGGGATAG
ATGTAAATGGACCAAAAGCACAGCTGATGTTGCGGTATCCAGATGGAAAA
AGGGAACAGATCACTCTTCCAGAGCAAGCTAAACTGCTAGCTTTGGTGAA
GCACGTGCAGTCTAAAGGATACCCAAATGAACGTTTTGAACTTCTCACCA
ACTTTCCTCGAAGGAAATTATCTCATCTGGACTATGATATTACATTGCAA
GAGGCAGGCCTTTGTCCTCAAGAGACTGTCTTTGTACAGGAAAGAAATTA
AGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQDHPPKSDENLYFQGGSDYKDDDDKGSAAHGGSAASSALKGLIQQFTTITGASESVGKHMLEACNNNLEMAVTMFLDGGGIAEEPSTSSASVSTVRPHTEEEVRAPIPQKQEILVEPEPLFGAPKRRRPARSIFDGFRDFQTETIRQEQELRNGGAIDKKLTHGQLDGLSSSPPKKCARSESLIDASEDSQLEAAIRASLQETHFDSTQTKQDSRSDEESESELFSGSEEFISVCGSDEEEEVENLAKSRKSPHKDLGHRKEENRRPLTEPPVRTDPGTATNHQGLPAVDSEILEMPPEKADGVVEGIDVNGPKAQLMLRYPDGKREQITLPEQAKLLALVKHVQSKGYPNERFELLTNFPRRKLSHLDYDITLQEAGLCPQETVFVQERN

• Vector Type: Bacterial
• DU Number: DU21295
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: UAS domain spans from amino acid 137 to 260 inclusive.; Differs from reference sequence in a single silent mutation only – a834c; ; ; Estimated MW of expressed protein : 69471 Daltons