cDNA Clone Ubiquitin (yeast)

• Plasmid: pGEX-4T-1 C-8Gly Uniquitin (Yeast)
• Parent Plasmid: pGEX4T1 C8Gly
• Expressed: GST C-8Gly
• Tag: GST
• Species: Saccharomyces cerevisiae

Sequence of Insert

GCGGATCCATGCAGATTTTCGTCAAGACTTTGACCGGTAAAACCATAACATTGGAAGTTGAATCTTCCGATACCATCGACAACGTTAAGTCGAAAATTCAAGACAAGGAAGGTATCCCTCCAGATCAACAAAGATTGATCTTTGCCGGTAAGCAGCTAGAAGACGGTAGAACGCTGTCTGATTACAACATTCAGAAGGAGTCCACCTTACATCTTGTGCTAAGGCTAAGAGGTGGTTAAGCGGCCGCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSCGGGGGGGGSMQIFVKTLTGKTIT
LEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKE
STLHLVLRLRGG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU21306
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: GST – Thrombin – C 8Gly – Ubiquitin ; ; Estimated MW of expressed protein : 35496 Daltons