cDNA Clone USP11

• Plasmid: pMalP USP11
• Parent Plasmid: pMAL6P
• Expressed: MBP-USP11
• Genbank: NM_004651
• Tag: MBP
• Species: Human

Sequence of Insert

GCGGCCGCTATGGCAGTAGCCCCGCGACTGTTTGGGGGGCTCTGCTTCCGT
TTCCGGGACCAGAATCCGGAAGTGGCTGTTGAGGGGCGTCTTCCAATCTC
GCACAGCTGCGTTGGCTGTAGAAGAGAACGGACGGCGATGGCGACGGTCG
CAGCAAATCCAGCTGCTGCTGCGGCGGCTGTGGCGGCGGCAGCGGCGGTG
ACTGAGGATAGAGAGCCACAGCACGAGGAGCTGCCAGGCCTGGACAGCCA
GTGGCGCCAGATAGAAAACGGCGAGAGTGGGCGAGAACGTCCACTGCGGG
CCGGCGAAAGCTGGTTCCTTGTGGAGAAGCACTGGTATAAGCAGTGGGAG
GCATACGTGCAGGGAGGGGACCAGGACTCCAGCACCTTCCCTGGCTGCAT
CAACAATGCCACACTCTTTCAAGATGAGATAAACTGGCGCCTCAAGGAGG
GACTGGTGGAAGGCGAGGATTATGTGCTGCTCCCAGCAGCTGCTTGGCAT
TACCTGGTCAGCTGGTATGGTCTAGAGCATGGCCAGCCACCCATTGAACG
CAAGGTCATAGAGCTGCCCAACATCCAGAAGGTCGAAGTGTACCCAGTAG
AACTGCTGCTTGTCCGGCACAATGATTTGGGCAAATCTCACACTGTTCAG
TTCAGCCATACCGATTCTATTGGCCTAGTATTGCGCACAGCTCGGGAGCG
GTTTCTGGTGGAGCCCCAGGAAGACACTCGGCTTTGGGCCAAGAACTCAG
AAGGCTCTTTGGATAGGTTGTATGACACACACATCACGGTTCTCGATGCG
GCCCTTGAGACTGGGCAGTTGATCATCATGGAGACCCGCAAGAAAGATGG
CACTTGGCCCAGCGCACAGCTGCATGTCATGAACAACAACATGTCGGAAG
AGGATGAGGACTTCAAGGGTCAGCCAGGCATCTGTGGCCTCACCAATCTG
GGCAACACGTGCTTCATGAACTCGGCCCTGCAGTGCCTCAGCAATGTGCC
ACAGCTCACCGAGTACTTCCTCAACAACTGCTACCTGGAGGAGCTCAACT
TCCGCAACCCACTGGGCATGAAGGGTGAGATCGCAGAGGCCTATGCAGAC
CTGGTGAAGCAGGCGTGGTCTGGCCACCACCGCTCCATTGTGCCACATGT
GTTCAAGAACAAGGTTGGCCATTTTGCATCCCAATTTCTGGGCTACCAGC
AGCATGACTCTCAGGAGCTGCTGTCATTCCTCCTGGACGGGCTGCATGAG
GACCTTAATCGGGTGAAGAAGAAGGAGTATGTGGAGCTGTGCGATGCTGC
TGGGCGACCGGATCAGGAGGTGGCACAGGAGGCATGGCAAAACCACAAAC
GGCGGAACGATTCTGTGATCGTGGACACTTTCCACGGCCTCTTCAAGTCC
ACGCTGGTGTGCCCCGATTGTGGCAATGTATCTGTGACCTTCGACCCCTT
CTGCTACCTCAGTGTTCCACTGCCTATCAGCCACAAGAGGGTCTTGGAGG
TCTTCTTTATCCCCATGGATCCGCGCCGCAAGCCAGAGCAGCACCGGCTC
GTGGTCCCCAAGAAAGGCAAGATCTCGGATCTATGTGTGGCTCTGTCCAA
ACACACGGGCATCTCGCCAGAGAGGATGATGGTGGCTGATGTCTTCAGTC
ACCGCTTCTATAAGCTCTATCAGCTAGAGGAGCCTCTGAGCAGCATCTTG
GACCGTGATGATATCTTCGTCTATGAGGTGTCAGGTCGCATTGAGGCCAT
TGAGGGCTCAAGAGAGGACATCGTGGTTCCTGTCTACCTGCGGGAGCGCA
CCCCTGCCCGTGACTACAACAACTCCTACTACGGCCTGATGCTTTTTGGA
CACCCCCTCCTGGTATCAGTGCCCCGGGACCGCTTCACCTGGGAGGGCCT
GTATAACGTCCTGATGTACCGGCTCTCACGCTACGTGACCAAACCCAACT
CAGATGATGAGGACGATGGGGATGAGAAAGAAGATGACGAGGAGGATAAA
GATGACGTCCCTGGGCCCTCAACTGGGGGCAGCCTCCGAGACCCTGAGCC
AGAGCAGGCTGGGCCCAGCTCTGGAGTCACGAACAGGTGCCCGTTCCTCC
TGGACAATTGCCTTGGCACATCTCAGTGGCCCCCAAGGCGACGACGCAAG
CAGCTGTTCACCCTGCAGACGGTGAACTCCAATGGGACCAGCGACCGCAC
AACCTCCCCTGAAGAAGTCCATGCCCAGCCGTACATTGCTATCGACTGGG
AGCCAGAGATGAAGAAGCGTTACTATGACGAGGTAGAGGCTGAGGGCTAC
GTGAAGCATGACTGCGTCGGGTACGTGATGAAGAAGGCTCCCGTGCGGCT
GCAGGAGTGCATTGAGCTCTTCACCACTGTGGAGACCCTGGAGAAGGAAA
ACCCCTGGTACTGCCCTTCCTGCAAGCAGCACCAGCTGGCAACCAAGAAG
CTGGACCTGTGGATGCTGCCGGAGATTCTCATCATCCACCTGAAACGCTT
TTCCTACACCAAGTTCTCCCGAGAGAAGCTGGACACCCTCGTGGAGTTTC
CTATCCGGGACCTGGACTTCTCTGAGTTTGTCATCCAGCCACAGAATGAG
TCGAATCCGGAGCTGTACAAATATGACCTCATCGCGGTTTCCAACCATTA
TGGGGGCATGCGTGATGGACACTACACAACATTTGCCTGCAACAAGGACA
GCGGCCAGTGGCACTACTTTGATGACAACAGCGTCTCCCCTGTCAATGAG
AATCAGATCGAGTCCAAGGCAGCCTATGTCCTCTTCTACCAACGCCAGGA
CGTGGCGCGACGCCTGCTGTCCCCGGCCGGCTCATCTGGCGCCCCAGCCT
CCCCTGCCTGCAGCTCCCCACCCAGCTCTGAGTTCATGGATGTTAATTGA
GCGGCCGC

Amino Acid Sequence

MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVG
KKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSG
LLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPN
PPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENG
KYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAM
TINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENA
QKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNN
NNNNNNLGIEGRISELALEVLFQGPWGSPEFPGVDSAAAMAVAPRLFGGL
CFRFRDQNPEVAVEGRLPISHSCVGCRRERTAMATVAANPAAAAAAVAAA
AAVTEDREPQHEELPGLDSQWRQIENGESGRERPLRAGESWFLVEKHWYK
QWEAYVQGGDQDSSTFPGCINNATLFQDEINWRLKEGLVEGEDYVLLPAA
AWHYLVSWYGLEHGQPPIERKVIELPNIQKVEVYPVELLLVRHNDLGKSH
TVQFSHTDSIGLVLRTARERFLVEPQEDTRLWAKNSEGSLDRLYDTHITV
LDAALETGQLIIMETRKKDGTWPSAQLHVMNNNMSEEDEDFKGQPGICGL
TNLGNTCFMNSALQCLSNVPQLTEYFLNNCYLEELNFRNPLGMKGEIAEA
YADLVKQAWSGHHRSIVPHVFKNKVGHFASQFLGYQQHDSQELLSFLLDG
LHEDLNRVKKKEYVELCDAAGRPDQEVAQEAWQNHKRRNDSVIVDTFHGL
FKSTLVCPDCGNVSVTFDPFCYLSVPLPISHKRVLEVFFIPMDPRRKPEQ
HRLVVPKKGKISDLCVALSKHTGISPERMMVADVFSHRFYKLYQLEEPLS
SILDRDDIFVYEVSGRIEAIEGSREDIVVPVYLRERTPARDYNNSYYGLM
LFGHPLLVSVPRDRFTWEGLYNVLMYRLSRYVTKPNSDDEDDGDEKEDDE
EDKDDVPGPSTGGSLRDPEPEQAGPSSGVTNRCPFLLDNCLGTSQWPPRR
RRKQLFTLQTVNSNGTSDRTTSPEEVHAQPYIAIDWEPEMKKRYYDEVEA
EGYVKHDCVGYVMKKAPVRLQECIELFTTVETLEKENPWYCPSCKQHQLA
TKKLDLWMLPEILIIHLKRFSYTKFSREKLDTLVEFPIRDLDFSEFVIQP
QNESNPELYKYDLIAVSNHYGGMRDGHYTTFACNKDSGQWHYFDDNSVSP
VNENQIESKAAYVLFYQRQDVARRLLSPAGSSGAPASPACSSPPSSEFMD
VN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20009
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid to express USP11 with N-terminal MBP tag (contains preScission protease site); ; Estimated MW of expressed protein : 157601 Daltons