cDNA Clone UBC1

• Plasmid: pGEX6P-UBC1
• Parent Plasmid: pGEX6P1
• Expressed: GST-UBC1
• Genbank: NM_005339
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCCAACATCGCGGTGCAGCGAATCAAGCGGGAGTTCAAGGA
GGTGCTGAAGAGCGAGGAGACGAGCAAAAATCAAATTAAAGTAGATCTTG
TAGATGAGAATTTTACAGAATTAAGAGGAGAAATAGCAGGACCTCCAGAC
ACACCATATGAAGGAGGAAGATACCAACTAGAGATAAAAATACCAGAAAC
ATACCCATTTAATCCCCCTAAGGTCCGGTTTATCACTAAAATATGGCATC
CTAATATTAGTTCCGTCACAGGGGCTATTTGTTTGGATATCCTGAAAGAT
CAATGGGCAGCTGCAATGACTCTCCGCACGGTATTATTGTCATTGCAAGC
ACTATTGGCAGCTGCAGAGCCAGATGATCCACAGGATGCTGTAGTAGCAA
ATCAGTACAAACAAAATCCCGAAATGTTCAAACAGACAGCTCGACTTTGG
GCACATGTGTATGCTGGAGCACCAGTTTCTAGTCCAGAATACACCAAAAA
AATAGAAAACCTATGTGCTATGGGCTTTGATAGGAATGCAGTAATAGTGG
CCTTGTCTTCAAAATCATGGGATGTAGAGACTGCAACAGAATTGCTTCTG
AGTAACTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMANIAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRGEIAGPPDTPYEGGRYQLEIKIPETYPFNPPKVRFITKIWHPNISSVTGAICLDILKDQWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYAGAPVSSPEYTKKIENLCAMGFDRNAVIVALSSKSWDVETATELLLSN

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20013
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for Ubc1 (E2-25K) with N-terminal GST tag (contains preScission protease site); ; Estimated MW of expressed protein : 49231 Daltons