cDNA Clone ATM

• Plasmid: pEBG6P-1 ATM 2670-3056(end) (catalytic domain)
• Parent Plasmid: pEBG6P
• Expressed: GST-ATM 2670-3056(end) (catalytic domain)
• Genbank: NM_000051
• Tag: GST
• Species: Human

Sequence of Insert

actagtAAGGTGGACCACACAGGAGAATATGGAAATCTGGTGACTATACAGTCATTTAAAGCAGAATTTCGCTTAGCAGGAGGTGTAAATTTACCAAAAATAATAGATTGTGTAGGTTCCGATGGCAAGGAGAGGAGACAGCTTGTTAAGGGCCGTGATGACCTGAGACAAGATGCTGTCATGCAACAGGTCTTCCAGATGTGTAATACATTACTGCAGAGAAACACGGAAACTAGGAAGAGGAAATTAACTATCTGTACTTATAAGGTGGTTCCCCTCTCTCAGCGAAGTGGTGTTCTTGAATGG
TGCACAGGAACTGTCCCCATTGGTGAATTTCTTGTTAACAATGAAGATGG
TGCTCATAAAAGATACAGGCCAAATGATTTCAGTGCCTTTCAGTGCCAAA
AGAAAATGATGGAGGTGCAAAAAAAGTCTTTTGAAGAGAAATATGAAGTC
TTCATGGATGTTTGCCAAAATTTTCAACCAGTTTTCCGTTACTTCTGCAT
GGAAAAATTCTTGGATCCAGCTATTTGGTTTGAGAAGCGATTGGCTTATA
CGCGCAGTGTAGCTACTTCTTCTATTGTTGGTTACATACTTGGACTTGGT
GATAGACATGTACAGAATATCTTGATAAATGAGCAGTCAGCAGAACTTGT
ACATATAGATCTAGGTGTTGCTTTTGAACAGGGCAAAATCCTTCCTACTC
CTGAGACAGTTCCTTTTAGACTCACCAGAGATATTGTGGATGGCATGGGC
ATTACGGGTGTTGAAGGTGTCTTCAGAAGATGCTGTGAGAAAACCATGGA
AGTGATGAGAAACTCTCAGGAAACTCTGTTAACCATTGTAGAGGTCCTTC
TATATGATCCACTCTTTGACTGGACCATGAATCCTTTGAAAGCTTTGTAT
TTACAGCAGAGGCCGGAAGATGAAACTGAGCTTCACCCTACTCTGAATGC
AGATGACCAAGAATGCAAACGAAATCTCAGTGATATTGACCAGAGTTTCA
ACAAAGTAGCTGAACGTGTCTTAATGAGACTACAAGAGAAACTGAAAGGA
GTGGAAGAAGGCACTGTGCTCAGTGTTGGTGGACAAGTGAATTTGCTCAT
ACAGCAGGCCATAGACCCCAAAAATCTCAGCCGACTTTTCCCAGGATGGA
AAGCTTGGGTGTGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAA
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSKVDHTGEYGNL
VTIQSFKAEFRLAGGVNLPKIIDCVGSDGKERRQLVKGRDDLRQDAVMQQ
VFQMCNTLLQRNTETRKRKLTICTYKVVPLSQRSGVLEWCTGTVPIGEFL
VNNEDGAHKRYRPNDFSAFQCQKKMMEVQKKSFEEKYEVFMDVCQNFQPV
FRYFCMEKFLDPAIWFEKRLAYTRSVATSSIVGYILGLGDRHVQNILINE
QSAELVHIDLGVAFEQGKILPTPETVPFRLTRDIVDGMGITGVEGVFRRC
CEKTMEVMRNSQETLLTIVEVLLYDPLFDWTMNPLKALYLQQRPEDETEL
HPTLNADDQECKRNLSDIDQSFNKVAERVLMRLQEKLKGVEEGTVLSVGG
QVNLLIQQAIDPKNLSRLFPGWKAWV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5043
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express ATM CAT with N-terminal GST (contains precission protease site); Estimated MW of expressed protein : 72006 Daltons