cDNA Clone CDK4

• Plasmid: pGEX6P-1-CDK4.6His
• Parent Plasmid: pGEX6P1
• Expressed: GST-CDK4-6His
• Genbank: NM_000075
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGGCTACCTCTCGATATGAGCCAGTGGCTGAAATTGGTGTCGGTGCCTATGGGACAGTGTACAAGGCCCGTGATCCCCACAGTGGCCACTTTGTGGCCCTCAAGAGTGTGAGAGTCCCCAATGGAGGAGGAGGTGGAGGAGGCCTTCCCATCAGCACAGTTCGTGAGGTGGCTTTACTGAGGCGACTGGAGGCTTTTGAGCATCCCAATGTTGTCCGGCTGATGGACGTCTGTGCCACATCCCGAACTGACCGGGAGATCAAGGTAACCCTGGTGTTTGAGCATGTAGACCAGGACCTA
AGGACATATCTGGACAAGGCACCCCCACCAGGCTTGCCAGCCGAAACGAT
CAAGGATCTGATGCGCCAGTTTCTAAGAGGCCTAGATTTCCTTCATGCCA
ATTGCATCGTTCACCGAGATCTGAAGCCAGAGAACATTCTGGTGACAAGT
GGTGGAACAGTCAAGCTGGCTGACTTTGGCCTGGCCAGAATCTACAGCTA
CCAGATGGCACTTACACCCGTGGTTGTTACACTCTGGTACCGAGCTCCCG
AAGTTCTTCTGCAGTCCACATATGCAACACCTGTGGACATGTGGAGTGTT
GGCTGTATCTTTGCAGAGATGTTTCGTCGAAAGCCTCTCTTCTGTGGAAA
CTCTGAAGCCGACCAGTTGGGCAAAATCTTTGACCTGATTGGGCTGCCTC
CAGAGGATGACTGGCCTCGAGATGTATCCCTGCCCCGTGGAGCCTTTCCC
CCCAGAGGGCCCCGCCCAGTGCAGTCGGTGGTACCTGAGATGGAGGAGTC
GGGAGCACAGCTGCTGCTGGAAATGCTGACTTTTAACCCACACAAGCGAA
TCTCTGCCTTTCGAGCTCTGCAGCACTCTTATCTACATAAGGATGAAGGT
AATCCGGAGCACCATCACCATCACCATTGAggatcc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMATSRYEPVAEIGVGAYGT
VYKARDPHSGHFVALKSVRVPNGGGGGGGLPISTVREVALLRRLEAFEHP
NVVRLMDVCATSRTDREIKVTLVFEHVDQDLRTYLDKAPPPGLPAETIKD
LMRQFLRGLDFLHANCIVHRDLKPENILVTSGGTVKLADFGLARIYSYQM
ALTPVVVTLWYRAPEVLLQSTYATPVDMWSVGCIFAEMFRRKPLFCGNSE
ADQLGKIFDLIGLPPEDDWPRDVSLPRGAFPPRGPRPVQSVVPEMEESGA
QLLLEMLTFNPHKRISAFRALQHSYLHKDEGNPEHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5103
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; Estimated MW of expressed protein : 61337 Daltons