cDNA Clone VASP

• Plasmid: pEBG6P-1 VASP
• Parent Plasmid: pEBG6P
• Expressed: GST-VASP
• Genbank: NM_003370
• Tag: GST
• Species: Human

Sequence of Insert

ggatccACTAGTAACGGCCGCCAGTGTGCTGGAATTCGCCCTTCATACGA
TGTGCCAGATTACGCCAGCGAGACGGTCATCTGTTCCAGCCGGGCCACTGTGATGCTTTATGATGATGGCAACAAGCGATGGCTCCCTGCTGGCACGGGTCCCCAGGCCTTCAGCCGCGTCCAGATCTACCACAACCCCACGGCCAATTCCTTTCGCGTCGTGGGCCGGAAGATGCAGCCCGACCAGCAGGTGGTCATCAACTGTGCCATCGTCCGGGGTGTCAAGTATAACCAGGCCACCCCCAACTTCCATCAGTGGCGCGACGCTCGCCAGGTCTGGGGCCTCAACTTCGGCAGCAAGGAGGATGCGGCCCAGTTTGCCGCCGGCATGGCCAGTGCCCTAGAGGCGTTGGAAGGAGGTGGGCCCCCTCCACCCCCAGCACTTCCCACCTGGTCGGTCCCGAACGGCCCCTCCCCGGAGGAGGTGGAGCAGCAGAAAAGGCAGCAGCCCGGCCCGTCGGAGCACATAGAGCGCCGGGTCTCCAATGCAGGAGGCCCACCTGCTCCCCCCGCTGGGGGTCCACCCCCACCACCAGGACCTCCCCCTCCTCCAGGTCCCCCCCCACCCCCAGGTTTGCCCCCTTCGGGGGTCCCAGCTGCAGCGCACGGAGCAGGGGGAGGACCACCCCCTGCACCCCCTCTCCCGGCAGCACAGGGCCCTGG
TGGTGGGGGAGCTGGGGCCCCAGGCCTGGCCGCAGCTATTGCTGGAGCCA
AACTCAGGAAAGTCAGCAAGCAGGAGGAGGCCTCAGGGGGGCCCACAGCC
CCCAAAGCTGAGAGTGGTCGAAGCGGAGGTGGGGGACTCATGGAAGAGAT
GAACGCCATGCTGGCCCGGAGAAGGAAAGCCACGCAAGTTGGGGAGAAAA
CCCCCAAGGATGAATCTGCCAATCAGGAGGAGCCAGAGGCCAGAGTCCCG
GCCCAGAGTGAATCTGTGCGGAGACCCTGGGAGAAGAACAGCACAACCTT
GCCAAGGATGAAGTCGTCTTCTTCGGTGACCACTTCCGAGACCCAACCCT
GCACGCCCAGCTCCAGTGATTACTCGGACCTACAGAGGGTGAAACAGGAG
CTTCTGGAAGAGGTGAAGAAGGAATTGCAGAAAGTGAAAGAGGAAATCAT
TGAAGCCTTCGTCCAGGAGCTGAGGAAGCGGGGTTCTCCCTGAggatcc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSNGRQCAGIRPS
YDVPDYASETVICSSRATVMLYDDGNKRWLPAGTGPQAFSRVQIYHNPTA
NSFRVVGRKMQPDQQVVINCAIVRGVKYNQATPNFHQWRDARQVWGLNFG
SKEDAAQFAAGMASALEALEGGGPPPPPALPTWSVPNGPSPEEVEQQKRQ
QPGPSEHIERRVSNAGGPPAPPAGGPPPPPGPPPPPGPPPPPGLPPSGVP
AAAHGAGGGPPPAPPLPAAQGPGGGGAGAPGLAAAIAGAKLRKVSKQEEA
SGGPTAPKAESGRSGGGGLMEEMNAMLARRRKATQVGEKTPKDESANQEE
PEARVPAQSESVRRPWEKNSTTLPRMKSSSSVTTSETQPCTPSSSDYSDL
QRVKQELLEEVKKELQKVKEEIIEAFVQELRKRGSP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5170
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express VASP with N-terminal GST (contains precission protease site); ; Estimated MW of expressed protein : 69241 Daltons