cDNA Clone CLK2

• Plasmid: pEBG6P-1 CLK2
• Parent Plasmid: pEBG6P
• Expressed: GST-CLK2
• Genbank: P49760
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGCCGCATCCTCGAAGGTACCACTCCTCAGAGCGAGGCAGCCGGGGGAGTTACCGTGAACACTATCGGAGCCGAAAGCATAAGCGACGAAGAAGTCGCTCCTGGTCAAGTAGTAGTGACCGGACACGACGGCGTCGGCGAGAGGACAGCTACCATGTCCGTTCTCGAAGCAGTTATGATGATCGTTCGTCCGACCGGAGGGTGTATGACCGGCGATACTGTGGCAGCTACAGACGCAACGATTATAGCCGGGATCGGGGAGATGCCTACTATGACACAGACTATCGGCATTCCTATGAA
TATCAGCGGGAGAACAGCAGTTACCGCAGCCAGCGCAGCAGCCGGAGGAA
GCACAGACGGCGGAGGAGGCGCAGCCGGACATTTAGCCGCTCATCTTCGC
AGCACAGCAGCCGGAGAGCCAAGAGTGTAGAGGACGACGCTGAGGGCCAC
CTCATCTACCACGTCGGGGACTGGCTACAAGAGCGATATGAAATCGTTAG
CACCTTAGGAGAGGGGACCTTCGGCCGAGTTGTACAATGTGTTGACCATC
GCAGGGGTGGGGCTCGAGTTGCCCTGAAGATCATTAAGAATGTGGAGAAG
TACAAGGAAGCAGCTCGACTTGAGATCAACGTGCTAGAGAAAATCAATGA
GAAAGACCCTGACAACAAGAACCTCTGTGTCCAGATGTTTGACTGGTTTG
ACTACCATGGCCACATGTGTATCTCCTTTGAGCTTCTGGGCCTTAGCACC
TTCGATTTCCTCAAAGACAACAACTACCTGCCCTACCCCATCCACCAAGT
GCGCCACATGGCCTTCCAGCTGTGCCAGGCTGTCAAGTTCCTCCATGATA
ACAAGCTGACACATACAGACCTCAAGCCTGAAAATATTCTGTTTGTGAAT
TCAGACTATGAGCTCACCTACAACCTAGAGAAGAAGCGAGATGAGCGCAG
TGTGAAGAGCACAGCTGTGCGGGTGGTAGACTTTGGCAGTGCCACCTTTG
ACCATGAGCACCATAGCACCATTGTCTCCACTCGCCATTACCGAGCACCA
GAAGTCATCCTTGAGTTGGGCTGGTCACAGCCTTGTGATGTGTGGAGTAT
AGGCTGCATCATCTTTGAATACTATGTGGGATTCACCCTCTTCCAGACCC
ATGACAACAGAGAGCATCTAGCCATGATGGAAAGGATCTTGGGTCCTATC
CCTTCCCGGATGATCCGAAAGACAAGAAAGCAGAAATATTTTTACCGGGG
TCGCCTGGATTGGGATGAGAACACATCAGCTGGGCGCTATGTTCGTGAGA
ACTGCAAACCGCTGCGGCGGTATCTGACCTCAGAGGCAGAGGAACACCAC
CAGCTCTTCGATCTGATTGAAAGCATGCTAGAGTATGAACCAGCTAAGCG
GCTGACCTTGGGTGAAGCCCTTCAGCATCCTTTCTTCGCCCGCCTTCGGG
CTGAGCCGCCCAACAAGTTGTGGGACTCCAGTCGGGATATCAGTCGGTGA
gcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMPHPRRYHSSERG
SRGSYREHYRSRKHKRRRSRSWSSSSDRTRRRRREDSYHVRSRSSYDDRS
SDRRVYDRRYCGSYRRNDYSRDRGDAYYDTDYRHSYEYQRENSSYRSQRS
SRRKHRRRRRRSRTFSRSSSQHSSRRAKSVEDDAEGHLIYHVGDWLQERY
EIVSTLGEGTFGRVVQCVDHRRGGARVALKIIKNVEKYKEAARLEINVLE
KINEKDPDNKNLCVQMFDWFDYHGHMCISFELLGLSTFDFLKDNNYLPYP
IHQVRHMAFQLCQAVKFLHDNKLTHTDLKPENILFVNSDYELTYNLEKKR
DERSVKSTAVRVVDFGSATFDHEHHSTIVSTRHYRAPEVILELGWSQPCD
VWSIGCIIFEYYVGFTLFQTHDNREHLAMMERILGPIPSRMIRKTRKQKY
FYRGRLDWDENTSAGRYVRENCKPLRRYLTSEAEEHHQLFDLIESMLEYE
PAKRLTLGEALQHPFFARLRAEPPNKLWDSSRDISR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5180
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express with N-terminal GST (contains precission protease site); ; Estimated MW of expressed protein : 87468 Daltons