cDNA Clone CLK2

• Plasmid: pGEX6P-3-CLK2
• Parent Plasmid: pGEX6P3
• Expressed: GST-CLK2
• Genbank: P49760
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGCCGCATCCTCGAAGGTACCACTCCTCAGAGCGAGGCAGCCGGGGGAGTTACCGTGAACACTATCGGAGCCGAAAGCATAAGCGACGAAGAAGTCGCTCCTGGTCAAGTAGTAGTGACCGGACACGACGGCGTCGGCGAGAGGACAGCTACCATGTCCGTTCTCGAAGCAGTTATGATGATCGTTCGTCCGACCGGAGGGTGTATGACCGGCGATACTGTGGCAGCTACAGACGCAACGATTATAGCCGGGATCGGGGAGATGCCTACTATGACACAGACTATCGGCATTCCTATGAA
TATCAGCGGGAGAACAGCAGTTACCGCAGCCAGCGCAGCAGCCGGAGGAA
GCACAGACGGCGGAGGAGGCGCAGCCGGACATTTAGCCGCTCATCTTCGC
AGCACAGCAGCCGGAGAGCCAAGAGTGTAGAGGACGACGCTGAGGGCCAC
CTCATCTACCACGTCGGGGACTGGCTACAAGAGCGATATGAAATCGTTAG
CACCTTAGGAGAGGGGACCTTCGGCCGAGTTGTACAATGTGTTGACCATC
GCAGGGGTGGGGCTCGAGTTGCCCTGAAGATCATTAAGAATGTGGAGAAG
TACAAGGAAGCAGCTCGACTTGAGATCAACGTGCTAGAGAAAATCAATGA
GAAAGACCCTGACAACAAGAACCTCTGTGTCCAGATGTTTGACTGGTTTG
ACTACCATGGCCACATGTGTATCTCCTTTGAGCTTCTGGGCCTTAGCACC
TTCGATTTCCTCAAAGACAACAACTACCTGCCCTACCCCATCCACCAAGT
GCGCCACATGGCCTTCCAGCTGTGCCAGGCTGTCAAGTTCCTCCATGATA
ACAAGCTGACACATACAGACCTCAAGCCTGAAAATATTCTGTTTGTGAAT
TCAGACTATGAGCTCACCTACAACCTAGAGAAGAAGCGAGATGAGCGCAG
TGTGAAGAGCACAGCTGTGCGGGTGGTAGACTTTGGCAGTGCCACCTTTG
ACCATGAGCACCATAGCACCATTGTCTCCACTCGCCATTACCGAGCACCA
GAAGTCATCCTTGAGTTGGGCTGGTCACAGCCTTGTGATGTGTGGAGTAT
AGGCTGCATCATCTTTGAATACTATGTGGGATTCACCCTCTTCCAGACCC
ATGACAACAGAGAGCATCTAGCCATGATGGAAAGGATCTTGGGTCCTATC
CCTTCCCGGATGATCCGAAAGACAAGAAAGCAGAAATATTTTTACCGGGG
TCGCCTGGATTGGGATGAGAACACATCAGCTGGGCGCTATGTTCGTGAGA
ACTGCAAACCGCTGCGGCGGTATCTGACCTCAGAGGCAGAGGAACACCAC
CAGCTCTTCGATCTGATTGAAAGCATGCTAGAGTATGAACCAGCTAAGCG
GCTGACCTTGGGTGAAGCCCTTCAGCATCCTTTCTTCGCCCGCCTTCGGG
CTGAGCCGCCCAACAAGTTGTGGGACTCCAGTCGGGATATCAGTCGGTGA
gcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMPHPRRYHSSERGSRGSYR
EHYRSRKHKRRRSRSWSSSSDRTRRRRREDSYHVRSRSSYDDRSSDRRVY
DRRYCGSYRRNDYSRDRGDAYYDTDYRHSYEYQRENSSYRSQRSSRRKHR
RRRRRSRTFSRSSSQHSSRRAKSVEDDAEGHLIYHVGDWLQERYEIVSTL
GEGTFGRVVQCVDHRRGGARVALKIIKNVEKYKEAARLEINVLEKINEKD
PDNKNLCVQMFDWFDYHGHMCISFELLGLSTFDFLKDNNYLPYPIHQVRH
MAFQLCQAVKFLHDNKLTHTDLKPENILFVNSDYELTYNLEKKRDERSVK
STAVRVVDFGSATFDHEHHSTIVSTRHYRAPEVILELGWSQPCDVWSIGC
IIFEYYVGFTLFQTHDNREHLAMMERILGPIPSRMIRKTRKQKYFYRGRL
DWDENTSAGRYVRENCKPLRRYLTSEAEEHHQLFDLIESMLEYEPAKRLT
LGEALQHPFFARLRAEPPNKLWDSSRDISR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5188
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 86859 Daltons