cDNA Clone AS160

• Synonyms: TBC1D4
• Plasmid: pGBKT7 AS160(A)
• Parent Plasmid: pGBKT7
• Expressed: GAL4-DNA-BD AS160(A)
• Genbank: NM_014832
• Tag: GAL4 DNA-BD
• Species: Human

Sequence of Insert

GAATTCATGGAGCCGCCCAGCTGCATTCAGGATGAGCCGTTCCCGCACCCCCTGGAGCCCGAGCCGGGCGTCTCAGCTCAGCCCGGCCCCGGGAAGCCAAGCGATAAGCGGTTCCGGCTGTGGTACGTTGGGGGGTCGTGCCTGGACCACAGGACCACGCTGCCTATGCTGCCCTGGCTCATGGCCGAGATCCGCAGGCGCAGCCAGAAGCCCGAGGCGGGCGGCTGCGGGGCGCCGGCGGCCCGAGAGGTGATCCTGGTGCTCAGCGCGCCCTTCCTGCGTTGCGTCCCCGCGCCGGGCGCTGGG
GCCTCGGGGGGCACTAGTCCGTCGGCCACGCAGCCCAACCCGGCGGTATT
CATCTTCGAGCACAAGGCGCAGCATATCTCGCGCTTCATCCACAACAGCC
ACGACCTCACCTACTTTGCCTACCTGATCAAGGCGCAGCCCGACGACCCC
GAGTCGCAGATGGCCTGCCACGTTTTCCGCGCCACAGACCCCAGCCAGGT
TCCTGATGTTATTAGCAGCATAAGGCAATTATCTAAAGCGGCCATGAAAG
AGGATGCCAAACCCAGCAAAGATAATGAGGACGCCTTTTACAACTCTCAG
AAGTTCGAAGTCCTGTACTGTGGAAAGGTGACCGTGACCCACAAGAAGGC
CCCCTCAAGCCTCATCGATGACTGCATGGAGAAGTTCAGCCTGCACGAAC
AGCAGCGCCTGAAGATCCAAGGCGAGCAGCGCGGTCCGGACCCAGGAGAG
GACCTGGCTGACTTGGAGGTGGTGGTGCCCGGGTCCCCCGGAGACTGCCT
GCCGGAGGAGGCTGACGGCACCGACACCCACCTTGGCTTACCTGCCGGGG
CCAGCCAGCCTGCCCTGACCAGCTCTCGGGTCTGCTTCCCTGAGCGGATT
TTGGAAGATTCTGGCTTTGATGAGCAGCAGGAGTTTCGGTCTCGGTGCAG
CAGTGTCACCGGCGTGCAACGGAGAGTTCACGAGGGCAGCCAGAAATCCC
AGCCGCGACGGAGACACGCGAGCGCACCCAGTCACGTCCAGCCCTCGGAC
TCGGAGAAGAACAGGACCATGCTCTTCCAGGTTGGGCGATTTGAGATTAA
CCTTATCAGTCCAGACACTAAATCAGTTGTGCTAGAAAAGAATTTTAAAG
ATATCTCCCCTTGTTCTCAGGGTATAAAGCATGTGGATCACTTTGGCTTT
ATCTGCCGGGAGTCTCCAGAGCCTGGACTTAGCCAGTATATTTGTTATGT
ATTCCAGTGTGCCAGCGAATCTCTGGTTGATGAGGTAATGCTGACTCTGA
AACAGGCCTTCAGTACGGCGGCTGCCCTGCAGAGTGCCAAGACGCAGATT
AAACTGTGTGAGGCCTGCCCGATGCACTCTTTGCATAAGCTCTGTGAAAG
GATTGAAGGTCTCTACCCACCAAGAGCCAAGCTGGTGATACAGAGGCATC
TCTCATCACTGACAGATAATGAGCAAGCTGACATCTTTGAAAGAGTTCAG
AAAATGAAGCCAGTCAGTGACCAGGAAGAAAATGAACTTGTGATTTTACA
CCTGAGGCAGCTGTGTGAAGCCAAGCAGAAGACACACGTGCACATCGGGG
AAGGCCCTGCTACTATTTCAAATAGTACAATCCCAGAAAATGCAACAAGC
AGTGGAAGGTTCAAACTTGACATTCTGAAAAATAAAGCTAAGAGATCCTT
AACTAGCTCCCTGGAAAATATCTTCTCAAGGGGAGCTAACAGAATGAGAG
GTCGGCTTGGAAGTGTGGACAGTTTTGAACGGTCCAACAGTCTTGCTTCA
GAGAAGGACTACTCACCAGGGGATTCTCCACCAGGGACACCGCCAGCGTC
CCCACCGTCCTCAGCTTGGCAAACGTTTCCCGAAGAGGATTCCGACTCCC
CGCAGTTTCGAAGACGGGCACACACGTTCAGCCACCCACCTTCAAGCACA
AAGAGAAAGCTGAATTTGCAGGATGGGAGGGCTCAGGGTGTGCGTTCCCC
TCTGCTGAGGCAGAGCTCCAGTGAACAGTGCAGTGATGGAGAAGGGAGAA
AAAGGACCTCATCTACCTGCAGCAATGAGTCCCTAAGTGTGGGAGGAACC
TCTGTCACTCCTCGCCGGATCTCCTGGCGGCAGCGCATTTTCCTCAGGGT
TGCTTCTCCCATGAACAAATCTCCCTCAGCAATGCAACAGCAAGATGGAT
TGGACAGGAACGAGCTGCTGCCACTGTCCCCCCTCTCTCCAACCATGGAG
GAGGAACCGCTGGTTATATTCCTGTCTGGGGAGGATGACCCAGAAAAGAT
TGAAGAAAGAAAGAAATCAAAAGAACTGAGGAGCTTGTGGAGAAAAGCTA
TACACCAACAAATCTTGTTACTTCGAATGGAAAAAGAAAACCAGAAACTT
GAAGCAAGCAGAGATGAACTCCAGTCCAGAAAAGTTAAATTAGACTATGA
AGAAGTTGGTGCATGTCAGAAAGAGGTCTTAATAACTTGGGATAAGAAGT
TGTTAAACTGCAGAGCTAAAATCAGATGTGATATGGAAGATATTCATACT
CTTCTTAAAGAAGGAGTTCCCAAAAGTCGACGAGGAGAAATTTGGCAGTT
TCTGGCTTTACAGTACCGACTCAGACACAGATTGCCTAATAAACAACAGC
CTCCTGACATATCCTATAAGGAACTTTTGAGGCAGCTCACTGCTCAGCAG
CATGCGATTCTTGTGGATTTAGGAAGGACGTTTCCTACTCACCCTTACTT
TTCAGTACAGCTTGGGCCAGGACAGCTGTCACTGTTTAACCTCCTGAAAG
CCTATTCTTTGCTGGACAAAGAAGTGGGATACTGTCAGGGGATCAGCTTT
GTGGCTGGAGTCCTGCTTCTGCACATGAGTGAAGAGCAAGCCTTTGAAAT
GCTGAAATTCCTCATGTATGACCTCGGCTTCCGCAAGCAGTACAGACCTG
ACATGATGTCGCTGCAGATTCAAATGTACCAGCTGTCCAGGCTCCTTCAT
GACTATCACAGAGATCTCTACAATCACCTTGAAGAAAATGAAATCAGCCC
CAGTCTTTATGCTGCCCCCTGGTTCCTCACATTGTTTGCCTCTCAGTTTT
CATTAGGATTTGTAGCCAGAGTTTTTGATATTATTTTTCTTCAGGGAACT
GAAGTTATATTCAAGGTTGCACTCAGCCTACTGAGCAGCCAAGAGACACT
TATAATGGAATGTGAGAGCTTTGAAAATATTGTTGAGTTTCTTAAAAACA
CGCTACCTGATATGAATACCTCTGAAATGGAAAAAATTATTACCCAGGTT
TTTGAGATGGATATTTCTAAGCAGTTGCATGCCTATGAGGTGGAATATCA
TGTGCTACAGGATGAGCTTCAGGAATCTTCATATTCCTGTGAGGATAGTG
AAACTTTGGAGAAGCTGGAGAGGGCCAATAGCCAACTGAAAAGACAAAAC
ATGGACCTCCTAGAAAAATTACAGGTAGCTCATACTAAAATCCAGGCCTT
GGAATCAAACCTGGAAAATCTTTTGACGAGAGAGACCAAAATGAAGTCTT
TAATCCGGACCCTGGAACAAGAAAAAATGGCTTATCAAAAGACAGTGGAG
CAACTCCGGAAGCTGCTGCCCGCGGATGCTCTAGCCAATTGTGACCTGTT
GCTGAGAGACCTAAACTGCAACCCTAACAACAAAGCCAAGATAGGAAATA
AGCCATAAGGATCC

Amino Acid Sequence

MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKRSPLT
RAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQD
NVNKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPEF
VIRLTIGRAAIMEEQKLISEEDLHMAMEAEFMEPPSCIQDEPFPHPLEPEPGVSAQPGPGKPSDKRFRLWYVGGSCLDHRTTLPMLPWLMAEIRRRSQKPEAGGCGAPAAREVILVLSAPFLRCVPAPGAGASGGTSPSATQPNPAVFIFEHKAQHISRFIHNSHDLTYFAYLIKAQPDDPESQMACHVFRATDPSQVPDVISSIRQLSKAAMKEDAKPSKDNEDAFYNSQKFEVLYCGKVTVTHKKAPSSLIDDCMEKFSLHEQQRLKIQGEQRGPDPGEDLADLEVVVPGSPGDCLPEEADGTDTHLGLPAGASQPALTSSRVCFPERILEDSGFDEQQEFRSRCSSVTGVQRRVHEGSQKSQPRRRHASAPSHVQPSDSEKNRTMLFQVGRFEINLISPDTKSVVLEKNFKDISPCSQGIKHVDHFGFICRESPEPGLSQYICYVFQCASESLVDEVMLTLKQAFSTAAALQSAKTQIKLCEACPMHSLHKLCERIEGLYPPRAKLVIQRHLSSLTDNEQADIFERVQKMKPVSDQEENELVILHLRQLCEAKQKTHVHIGEGPATISNSTIPENATSSGRFKLDILKNKAKRSLTSSLENIFSRGANRMRGRLGSVDSFERSNSLASEKDYSPGDSPPGTPPASPPSSAWQTFPEEDSDSPQFRRRAHTFSHPPSSTKRKLNLQDGRAQGVRSPLLRQSSSEQCSDGEGRKRTSSTCSNESLSVGGTSVTPRRISWRQRIFLRVASPMNKSPSAMQQQDGLDRNELLPLSPLSPTMEEEPLVIFLSGEDDPEKIEERKKSKELRSLWRKAIHQQILLLRMEKENQKLEASRDELQSRKVKLDYEEVGACQKEVLITWDKKLLNCRAKIRCDMEDIHTLLKEGVPKSRRGEIWQFLALQYRLRHRLPNKQQPPDISYKELLRQLTAQQHAILVDLGRTFPTHPYFSVQLGPGQLSLFNLLKAYSLLDKEVGYCQGISFVAGVLLLHMSEEQAFEMLKFLMYDLGFRKQYRPDMMSLQIQMYQLSRLLHDYHRDLYNHLEENEISPSLYAAPWFLTLFASQFSLGFVARVFDIIFLQGTEVIFKVALSLLSSQETLIMECESFENIVEFLKNTLPDMNTSEMEKIITQVFEMDISKQLHAYEVEYHVLQDELQESSYSCEDSETLEKLERANSQLKRQNMDLLEKLQVAHTKIQALESNLENLLTRETKMKSLIRTLEQEKMAYQKTVEQLRKLLPADALANCDLLLRDLNCNPNNKAKIGNKP*

• Vector Type: Other
• Antibiotic: Kan
• DU Number: DU5799
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: MATCHMAKER yeast Two-Hybrid vector.; ; N.B. sequence differences in AS160 CDS compared with NM_014832:; T1159c (S387P); T1609g (S537A); Delta nt 2034-2222 (delta aa N679-S741) (The cloned sequence represents a splice variant lacking amino acids 679-741. The majority of ESTs in the NCBI database represent this form.); Delta nt 2594-2596 (delta aa G865); A2873g (K958R); C2904t (silemt L968)