cDNA Clone 14-3-3 zeta

• Synonyms: YWHAZ
• Plasmid: pETBlue2.6His 14-3-3 zeta R56A R60A
• Parent Plasmid: pETBlue2 6His
• Expressed: 6His-14-3-3 zeta R56A R60A
• Genbank: NM_145690
• Tag: 6His
• Species: Human

Sequence of Insert

agatctGATAAAAATGAGCTGGTTCAGAAGGCCAAACTGGCCGAGCAGGCTGAGCGATATGATGACATGGCAGCCTGCATGAAGTCTGTAACTGAGCAAGGAGCTGAATTATCCAATGAGGAGAGGAATCTTCTCTCAGTTGCTTATAAAAAT
GTTGTAGGAGCCCGTGCGTCATCTTGGGCGGTCGTCTCAAGTATTGAACA
AAAGACGGAAGGTGCTGAGAAAAAACAGCAGATGGCTCGAGAATACAGAG
AGAAAATTGAGACGGAGCTAAGAGATATCTGCAATGATGTACTGTCTCTT
TTGGAAAAGTTCTTGATCCCCAATGCTTCACAAGCAGAGAGCAAAGTCTT
CTATTTGAAAATGAAAGGAGATTACTACCGTTACTTGGCTGAGGTTGCCG
CTGGTGATGACAAGAAAGGGATTGTCGATCAGTCACAACAAGCATACCAA
GAAGCTTTTGAAATCAGCAAAAAGGAAATGCAACCAACACATCCTATCAG
ACTGGGTCTGGCCCTTAACTTCTCTGTGTTCTATTATGAGATTCTGAACT
CCCCAGAGAAAGCCTGCTCTCTTGCAAAGACAGCTTTTGATGAAGCCATT
GCTGAACTTGATACATTAAGTGAAGAGTCATACAAAGACAGCACGCTAAT
AATGCAATTACTGAGAGACAACTTGACATTGTGGACATCGGATACCCAAG
GAGACGAAGCTGAAGCAGGAGAAGGAGGGGAAAATTAAgaattc

Amino Acid Sequence

MGHHHHHHLGSDKNELVQKAKLAEQAERYDDMAACMKSVTEQGAELSNEE
RNLLSVAYKNVVGARASSWAVVSSIEQKTEGAEKKQQMAREYREKIETEL
RDICNDVLSLLEKFLIPNASQAESKVFYLKMKGDYYRYLAEVAAGDDKKG
IVDQSQQAYQEAFEISKKEMQPTHPIRLGLALNFSVFYYEILNSPEKACS
LAKTAFDEAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDTQGDEAEAG
EGGEN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5829
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Construct for bacterial expression of N-terminal 6His-tagged protein.