cDNA Clone CD133

• Plasmid: pCMV5-HA CD133
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-CD133
• Genbank: NM_006017
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGGCCCTCGTACTCGGCTCCCTGTTGCTGCTGGGGCTGTGCGGGAACTCCTTTTCAGGAGGGCAGCCTTCATCCACAGATGCTCCTAAGGCTTGGAATTATGAATTGCCTGCAACAAATTATGAGACCCAAGACTCCCATAAAGCTGGACCCATTGGCATTCTCTTTGAACTAGTGCATATCTTTCTCTATGTGGTACAGCCGCGTGATTTCCCAGAAGATACTTTGAGAAAATTCTTACAGAAGGCATATGAATCCAAAATTGATTATGACAAGCCAGAAACTGTAATCTTAGGTCTA
AAGATTGTCTACTATGAAGCAGGGATTATTCTATGCTGTGTCCTGGGGCT
GCTGTTTATTATTCTGATGCCTCTGGTGGGGTATTTCTTTTGTATGTGTC
GTTGCTGTAACAAATGTGGTGGAGAAATGCACCAGCGACAGAAGGAAAAT
GGGCCCTTCCTGAGGAAATGCTTTGCAATCTCCCTGTTGGTGATTTGTAT
AATAATAAGCATTGGCATCTTCTATGGTTTTGTGGCAAATCACCAGGTAA
GAACCCGGATCAAAAGGAGTCGGAAACTGGCAGATAGCAATTTCAAGGAC
TTGCGAACTCTCTTGAATGAAACTCCAGAGCAAATCAAATATATATTGGC
CCAGTACAACACTACCAAGGACAAGGCGTTCACAGATCTGAACAGTATCA
ATTCAGTGCTAGGAGGCGGAATTCTTGACCGACTGAGACCCAACATCATC
CCTGTTCTTGATGAGATTAAGTCCATGGCAACAGCGATCAAGGAGACCAA
AGAGGCGTTGGAGAACATGAACAGCACCTTGAAGAGCTTGCACCAACAAA
GTACACAGCTTAGCAGCAGTCTGACCAGCGTGAAAACTAGCCTGCGGTCA
TCTCTCAATGACCCTCTGTGCTTGGTGCATCCATCAAGTGAAACCTGCAA
CAGCATCAGATTGTCTCTAAGCCAGCTGAATAGCAACCCTGAACTGAGGC
AGCTTCCACCCGTGGATGCAGAACTTGACAACGTTAATAACGTTCTTAGG
ACAGATTTGGATGGCCTGGTCCAACAGGGCTATCAATCCCTTAATGATAT
ACCTGACAGAGTACAACGCCAAACCACGACTGTCGTAGCAGGTATCAAAA
GGGTCTTGAATTCCATTGGTTCAGATATCGACAATGTAACTCAGCGTCTT
CCTATTCAGGATATACTCTCAGCATTCTCTGTTTATGTTAATAACACTGA
AAGTTACATCCACAGAAATTTACCTACATTGGAAGAGTATGATTCATACT
GGTGGCTGGGTGGCCTGGTCATCTGCTCTCTGCTGACCCTCATCGTGATT
TTTTACTACCTGGGCTTACTGTGTGGCGTGTGCGGCTATGACAGGCATGC
CACCCCGACCACCCGAGGCTGTGTCTCCAACACCGGAGGCGTCTTCCTCA
TGGTTGGAGTTGGATTAAGTTTCCTCTTTTGCTGGATATTGATGATCATT
GTGGTTCTTACCTTTGTCTTTGGTGCAAATGTGGAAAAACTGATCTGTGA
ACCTTACACGAGCAAGGAATTATTCCGGGTTTTGGATACACCCTACTTAC
TAAATGAAGACTGGGAATACTATCTCTCTGGGAAGCTATTTAATAAATCA
AAAATGAAGCTCACTTTTGAACAAGTTTACAGTGACTGCAAAAAAAATAG
AGGCACTTACGGCACTCTTCACCTGCAGAACAGCTTCAATATCAGTGAAC
ATCTCAACATTAATGAGCATACTGGAAGCATAAGCAGTGAATTGGAAAGT
CTGAAGGTAAATCTTAATATCTTTCTGTTGGGTGCAGCAGGAAGAAAAAA
CCTTCAGGATTTTGCTGCTTGTGGAATAGACAGAATGAATTATGACAGCT
ACTTGGCTCAGACTGGTAAATCCCCCGCAGGAGTGAATCTTTTATCATTT
GCATATGATCTAGAAGCAAAAGCAAACAGTTTGCCCCCAGGAAATTTGAG
GAACTCCCTGAAAAGAGATGCACAAACTATTAAAACAATTCACCAGCAAC
GAGTCCTTCCTATAGAACAATCACTGAGCACTCTATACCAAAGCGTCAAG
ATACTTCAACGCACAGGGAATGGATTGTTGGAGAGAGTAACTAGGATTCT
AGCTTCTCTGGATTTTGCTCAGAACTTCATCACAAACAATACTTCCTCTG
TTATTATTGAGGAAACTAAGAAGTATGGGAGAACAATAATAGGATATTTT
GAACATTATCTGCAGTGGATCGAGTTCTCTATCAGTGAGAAAGTGGCATC
GTGCAAACCTGTGGCCACCGCTCTAGATACTGCTGTTGATGTCTTTCTGT
GTAGCTACATTATCGACCCCTTGAATTTGTTTTGGTTTGGCATAGGAAAA
GCTACTGTATTTTTACTTCCGGCTCTAATTTTTGCGGTAAAACTGGCTAA
GTACTATCGTCGAATGGATTCGGAGGACGTGTACGATGATGTTGAAACTA
TACCCATGAAAAATATGGAAAATGGTAATAATGGTTATCATAAAGATCAT
GTATATGGTATTCACAATCCTGTTATGACAAGCCCATCACAACATTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMALVLGSLLLLGLCGNSFSGGQPSSTDAPKAWNYELPA
TNYETQDSHKAGPIGILFELVHIFLYVVQPRDFPEDTLRKFLQKAYESKI
DYDKPETVILGLKIVYYEAGIILCCVLGLLFIILMPLVGYFFCMCRCCNK
CGGEMHQRQKENGPFLRKCFAISLLVICIIISIGIFYGFVANHQVRTRIK
RSRKLADSNFKDLRTLLNETPEQIKYILAQYNTTKDKAFTDLNSINSVLG
GGILDRLRPNIIPVLDEIKSMATAIKETKEALENMNSTLKSLHQQSTQLS
SSLTSVKTSLRSSLNDPLCLVHPSSETCNSIRLSLSQLNSNPELRQLPPV
DAELDNVNNVLRTDLDGLVQQGYQSLNDIPDRVQRQTTTVVAGIKRVLNS
IGSDIDNVTQRLPIQDILSAFSVYVNNTESYIHRNLPTLEEYDSYWWLGG
LVICSLLTLIVIFYYLGLLCGVCGYDRHATPTTRGCVSNTGGVFLMVGVG
LSFLFCWILMIIVVLTFVFGANVEKLICEPYTSKELFRVLDTPYLLNEDW
EYYLSGKLFNKSKMKLTFEQVYSDCKKNRGTYGTLHLQNSFNISEHLNIN
EHTGSISSELESLKVNLNIFLLGAAGRKNLQDFAACGIDRMNYDSYLAQT
GKSPAGVNLLSFAYDLEAKANSLPPGNLRNSLKRDAQTIKTIHQQRVLPI
EQSLSTLYQSVKILQRTGNGLLERVTRILASLDFAQNFITNNTSSVIIEE
TKKYGRTIIGYFEHYLQWIEFSISEKVASCKPVATALDTAVDVFLCSYII
DPLNLFWFGIGKATVFLLPALIFAVKLAKYYRRMDSEDVYDDVETIPMKN
MENGNNGYHKDHVYGIHNPVMTSPSQH*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11215
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 98559 Daltons