cDNA Clone AS160

• Synonyms: TBC1D4
• Plasmid: pCMV5-FLAG AS160 (B)
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG-AS160(B)
• Genbank: NM_014832
• Tag: FLAG
• Species: Human

Sequence of Insert

ggatccATGGAGCCGCCCAGCTGCATTCAGGATGAGCCGTTCCCGCACCC
CCTGGAGCCCGAGCCGGGCGTCTCAGCTCAGCCCGGCCCCGGGAAGCCAA
GCGATAAGCGGTTCCGGCTGTGGTACGTTGGGGGGTCGTGCCTGGACCAC
AGGACCACGCTGCCTATGCTGCCCTGGCTCATGGCCGAGATCCGCAGGCG
CAGCCAGAAGCCCGAGGCGGGCGGCTGCGGGGCGCCGGCGGCCCGAGAGG
TGATCCTGGTGCTCAGCGCGCCCTTCCTGCGTTGCGTCCCCGCGCCGGGC
GCTGGGGCCTCGGGGGGCACTAGTCCGTCGGCCACGCAGCCCAACCCGGC
GGTATTCATCTTCGAGCACAAGGCGCAGCATATCTCGCGCTTCATCCACA
ACAGCCACGACCTCACCTACTTTGCCTACCTGATCAAGGCGCAGCCCGAC
GACCCCGAGTCGCAGATGGCCTGCCACGTTTTCCGCGCCACAGACCCCAG
CCAGGTTCCTGATGTTATTAGCAGCATAAGGCAATTATCTAAAGCGGCCA
TGAAAGAGGATGCCAAACCCAGCAAAGATAATGAGGACGCCTTTTACAAC
TCTCAGAAGTTCGAAGTCCTGTACTGTGGAAAGGTGACCGTGACCCACAA
GAAGGCCCCCTCAAGCCTCATCGATGACTGCATGGAGAAGTTCAGCCTGC
ACGAACAGCAGCGCCTGAAGATCCAAGGCGAGCAGCGCGGTCCGGACCCA
GGAGAGGACCTGGCTGACTTGGAGGTGGTGGTGCCCGGGTCCCCCGGAGA
CTGCCTGCCGGAGGAGGCTGACGGCACCGACACCCACCTTGGCTTACCTG
CCGGGGCCAGCCAGCCTGCCCTGACCAGCTCTCGGGTCTGCTTCCCTGAG
CGGATTTTGGAAGATTCTGGCTTTGATGAGCAGCAGGAGTTTCGGTCTCG
GTGCAGCAGTGTCACCGGCGTGCAACGGAGAGTTCACGAGGGCAGCCAGA
AATCCCAGCCGCGACGGAGACACGCGAGCGCACCCAGTCACGTCCAGCCC
TCGGACTCGGAGAAGAACAGGACCATGCTCTTCCAGGTTGGGCGATTTGA
GATTAACCTTATCAGTCCAGACACTAAATCAGTTGTGCTAGAAAAGAATT
TTAAAGATATCTCCCCTTGTTCTCAGGGTATAAAGCATGTGGATCACTTT
GGCTTTATCTGCCGGGAGTCTCCAGAGCCTGGACTTAGCCAGTATATTTG
TTATGTATTCCAGTGTGCCAGCGAATCTCTGGTTGATGAGGTAATGCTGA
CTCTGAAACAGGCCTTCAGTACGGCGGCTGCCCTGCAGAGTGCCAAGACG
CAGATTAAACTGTGTGAGGCCTGCCCGATGCACTCTTTGCATAAGCTCTG
TGAAAGGATTGAAGGTCTCTACCCACCAAGAGCCAAGCTGGTGATACAGA
GGCATCTCTCATCACTGACAGATAATGAGCAAGCTGACATCTTTGAAAGA
GTTCAGAAAATGAAGCCAGTCAGTGACCAGGAAGAAAATGAACTTGTGAT
TTTACACCTGAGGCAGCTGTGTGAAGCCAAGCAGAAGACACACGTGCACA
TCGGGGAAGGCCCTGCTACTATTTCAAATAGTACAATCCCAGAAAATGCA
ACAAGCAGTGGAAGGTTCAAACTTGACATTCTGAAAAATAAAGCTAAGAG
ATCCTTAACTAGCTCCCTGGAAAATATCTTCTCAAGGGGAGCTAACAGAA
TGAGAGGTCGGCTTGGAAGTGTGGACAGTTTTGAACGGTCCAACAGTCTT
GCTTCAGAGAAGGACTACTCACCAGGGGATTCTCCACCAGGGACACCGCC
AGCGTCCCCACCGTCCTCAGCTTGGCAAACGTTTCCCGAAGAGGATTCCG
ACTCCCCGCAGTTTCGAAGACGGGCACACACGTTCAGCCACCCACCTTCA
AGCACAAAGAGAAAGCTGAATTTGCAGGATGGGAGGGCTCAGGGTGTGCG
TTCCCCTCTGCTGAGGCAGAGCTCCAGTGAACAGTGCAGCAATCTTTCGT
CAGTTCGACGCATGTACAAGGAGAGTAATTCTTCCTCCAGTCTTCCAAGT
CTTCACACTTCCTTCTCTGCCCCTTCCTTCACTGCCCCCTCTTTCCTGAA
AAGCTTTTACCAGAATTCAGGTAGACTGTCCCCACAGTATGAAAATGAAA
TCAGTGATGGAGAAGGGAGAAAAAGGACCTCATCTACCTGCAGCAATGAG
TCCCTAAGTGTGGGAGGAACCTCTGTCACTCCTCGCCGGATCTCCTGGCG
GCAGCGCATTTTCCTCAGGGTTGCTTCTCCCATGAACAAATCTCCCTCAG
CAATGCAACAGCAAGATGGATTGGACAGGAACGAGCTGCTGCCACTGTCC
CCCCTCTCTCCAACCATGGAGGAGGAACCGCTGGTTATATTCCTGTCTGG
GGAGGATGACCCAGAAAAGATTGAAGAAAGAAAGAAATCAAAAGAACTGA
GGAGCTTGTGGAGAAAAGCTATACACCAACAAATCTTGTTACTTCGAATG
GAAAAAGAAAACCAGAAACTTGAAGCAAGCAGAGATGAACTCCAGTCCAG
AAAAGTTAAATTAGACTATGAAGAAGTTGGTGCATGTCAGAAAGAGGTCT
TAATAACTTGGGATAAGAAGTTGTTAAACTGCAGAGCTAAAATCAGATGT
GATATGGAAGATATTCATACTCTTCTTAAAGAAGGAGTTCCCAAAAGTCG
ACGAGGAGAAATTTGGCAGTTTCTGGCTTTACAGTACCGACTCAGACACA
GATTGCCTAATAAACAACAGCCTCCTGACATATCCTATAAGGAACTTTTG
AGGCAGCTCACTGCTCAGCAGCATGCGATTCTTGTGGATTTAGGAAGGAC
GTTTCCTACTCACCCTTACTTTTCAGTACAGCTTGGGCCAGGACAGCTGT
CACTGTTTAACCTCCTGAAAGCCTATTCTTTGCTGGACAAAGAAGTGGGA
TACTGTCAGGGGATCAGCTTTGTGGCTGGAGTCCTGCTTCTGCACATGAG
TGAAGAGCAAGCCTTTGAAATGCTGAAATTCCTCATGTATGACCTCGGCT
TCCGCAAGCAGTACAGACCTGACATGATGTCGCTGCAGATTCAAATGTAC
CAGCTGTCCAGGCTCCTTCATGACTATCACAGAGATCTCTACAATCACCT
TGAAGAAAATGAAATCAGCCCCAGTCTTTATGCTGCCCCCTGGTTCCTCA
CATTGTTTGCCTCTCAGTTTTCATTAGGATTTGTAGCCAGAGTTTTTGAT
ATTATTTTTCTTCAGGGAACTGAAGTTATATTCAAGGTTGCACTCAGCCT
ACTGAGCAGCCAAGAGACACTTATAATGGAATGTGAGAGCTTTGAAAATA
TTGTTGAGTTTCTTAAAAACACGCTACCTGATATGAATACCTCTGAAATG
GAAAAAATTATTACCCAGGTTTTTGAGATGGATATTTCTAAGCAGTTGCA
TGCCTATGAGGTGGAATATCATGTGCTACAGGATGAGCTTCAGGAATCTT
CATATTCCTGTGAGGATAGTGAAACTTTGGAGAAGCTGGAGAGGGCCAAT
AGCCAACTGAAAAGACAAAACATGGACCTCCTAGAAAAATTACAGGTAGC
TCATACTAAAATCCAGGCCTTGGAATCAAACCTGGAAAATCTTTTGACGA
GAGAGACCAAAATGAAGTCTTTAATCCGGACCCTGGAACAAGAAAAAATG
GCTTATCAAAAGACAGTGGAGCAACTCCGGAAGCTGCTGCCCGCGGATGC
TCTAGCCAATTGTGACCTGTTGCTGAGAGACCTAAACTGCAACCCTAACA
ACAAAGCCAAGATAGGAAATAAGCCATAAggatcc

Amino Acid Sequence

MDYKDDDDKGSMEPPSCIQDEPFPHPLEPEPGVSAQPGPGKPSDKRFRLW
YVGGSCLDHRTTLPMLPWLMAEIRRRSQKPEAGGCGAPAAREVILVLSAP
FLRCVPAPGAGASGGTSPSATQPNPAVFIFEHKAQHISRFIHNSHDLTYF
AYLIKAQPDDPESQMACHVFRATDPSQVPDVISSIRQLSKAAMKEDAKPS
KDNEDAFYNSQKFEVLYCGKVTVTHKKAPSSLIDDCMEKFSLHEQQRLKI
QGEQRGPDPGEDLADLEVVVPGSPGDCLPEEADGTDTHLGLPAGASQPAL
TSSRVCFPERILEDSGFDEQQEFRSRCSSVTGVQRRVHEGSQKSQPRRRH
ASAPSHVQPSDSEKNRTMLFQVGRFEINLISPDTKSVVLEKNFKDISPCS
QGIKHVDHFGFICRESPEPGLSQYICYVFQCASESLVDEVMLTLKQAFST
AAALQSAKTQIKLCEACPMHSLHKLCERIEGLYPPRAKLVIQRHLSSLTD
NEQADIFERVQKMKPVSDQEENELVILHLRQLCEAKQKTHVHIGEGPATI
SNSTIPENATSSGRFKLDILKNKAKRSLTSSLENIFSRGANRMRGRLGSV
DSFERSNSLASEKDYSPGDSPPGTPPASPPSSAWQTFPEEDSDSPQFRRR
AHTFSHPPSSTKRKLNLQDGRAQGVRSPLLRQSSSEQCSNLSSVRRMYKE
SNSSSSLPSLHTSFSAPSFTAPSFLKSFYQNSGRLSPQYENEISDGEGRK
RTSSTCSNESLSVGGTSVTPRRISWRQRIFLRVASPMNKSPSAMQQQDGL
DRNELLPLSPLSPTMEEEPLVIFLSGEDDPEKIEERKKSKELRSLWRKAI
HQQILLLRMEKENQKLEASRDELQSRKVKLDYEEVGACQKEVLITWDKKL
LNCRAKIRCDMEDIHTLLKEGVPKSRRGEIWQFLALQYRLRHRLPNKQQP
PDISYKELLRQLTAQQHAILVDLGRTFPTHPYFSVQLGPGQLSLFNLLKA
YSLLDKEVGYCQGISFVAGVLLLHMSEEQAFEMLKFLMYDLGFRKQYRPD
MMSLQIQMYQLSRLLHDYHRDLYNHLEENEISPSLYAAPWFLTLFASQFS
LGFVARVFDIIFLQGTEVIFKVALSLLSSQETLIMECESFENIVEFLKNT
LPDMNTSEMEKIITQVFEMDISKQLHAYEVEYHVLQDELQESSYSCEDSE
TLEKLERANSQLKRQNMDLLEKLQVAHTKIQALESNLENLLTRETKMKSL
IRTLEQEKMAYQKTVEQLRKLLPADALANCDLLLRDLNCNPNNKAKIGNK
P*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11223
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence differences in cloned AS160 (B) (long form) compared with NM_014832: ; S387P, S537A, K958R, delta R733-S740, delta G865. These differences are present in all AS160 (long form) DU clones.; ; Estimated MW of expressed protein : 146962 Daltons