cDNA Clone CHK1

• Synonyms: CHEK1
• Plasmid: pAS2.6 CHK1 D148A
• Parent Plasmid: pAS2.6
• Expressed: GAL4-DNA-BD CHK1 D148A
• Genbank: BC004202
• Tag: GAL4 DNA-BD
• Species: Human

Sequence of Insert

GAATTCATGGCAGTGCCCTTTGTGGAAGACTGGGACTTGGTGCAAACCCTGGGAGAAGGTGCCTATGGAGAAGTTCAACTTGCTGTGAATAGAGTAACTGAAGAAGCAGTCGCAGTGAAGATTGTAGATATGAAGCGTGCCGTAGACTGTCCAGAAAATATTAAGAAAGAGATCTGTATCAATAAAATGCTAAATCATGAAAATGTAGTAAAATTCTATGGTCACAGGAGAGAAGGCAATATCCAATATTTATTTCTGGAGTACTGTAGTGGAGGAGAGCTTTTTGACAGAATAGAGCCAGACATA
GGCATGCCTGAACCAGATGCTCAGAGATTCTTCCATCAACTCATGGCAGG
GGTGGTTTATCTGCATGGTATTGGAATAACTCACAGGGATATTAAACCAG
AAAATCTTCTGTTGGATGAAAGGGATAACCTCAAAATCTCAGCCTTTGGC
TTGGCAACAGTATTTCGGTATAATAATCGTGAGCGTTTGTTGAACAAGAT
GTGTGGTACTTTACCATATGTTGCTCCAGAACTTCTGAAGAGAAGAGAAT
TTCATGCGGAACCAGTTGATGTTTGGTCCTGTGGAATAGTACTTACTGCA
ATGCTCGCTGGAGAATTGCCATGGGACCAACCCAGTGACAGCTGTCAGGA
GTATTCTGACTGGAAAGAAAAAAAAACATACCTCAACCCTTGGAAAAAAA
TCGATTCTGCTCCTCTAGCTCTGCTGCATAAAATCTTAGTTGAGAATCCA
TCAGCAAGAATTACCATTCCAGACATCAAAAAAGATAGATGGTACAACAA
ACCCCTCAAGAAAGGGGCAAAAAGGCCCCGAGTCACTTCAGGTGGTGTGT
CAGAGTCTCCCAGTGGATTTTCTAAGCACATTCAATCCAATTTGGACTTC
TCTCCAGTAAACAGTGCTTCTAGTGAAGAAAATGTGAAGTACTCCAGTTC
TCAGCCAGAACCCCGCACAGGTCTTTCCTTATGGGATACCAGCCCCTCAT
ACATTGATAAATTGGTACAAGGGATCAGCTTTTCCCAGCCCACATGTCCT
GATCATATGCTTTTGAATAGTCAGTTACTTGGCACCCCAGGATCCTCACA
GAACCCCTGGCAGCGGTTGGTCAAAAGAATGACACGATTCTTTACCAAAT
TGGATGCAGACAAATCTTATCAATGCCTGAAAGAGACTTGTGAGAAGTTG
GGCTATCAATGGAAGAAAAGTTGTATGAATCAGGTTACTATATCAACAAC
TGATAGGAGAAACAATAAACTCATTTTCAAAGTGAATTTGTTAGAAATGG
ATGATAAAATATTGGTTGACTTCCGGCTTTCTAAGGGTGATGGATTGGAG
TTCAAGAGACACTTCCTGAAGATTAAAGGGAAGCTGATTGATATTGTGAG
CAGCCAGAAGGTTTGGCTTCCTGCCACATGAGCGGCCGC

Amino Acid Sequence

MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKRSPLT
RAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQD
NVNKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPVL
QYPALTHMGSPEFMAVPFVEDWDLVQTLGEGAYGEVQLAVNRVTEEAVAVKIVDMKRAVDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEPDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDERDNLKISAFGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRPRVTSGGVSESPSGFSKHIQSNLDFSPVNSASSEENVKYSSSQPEPRTGLSLWDTSPSYIDKLVQGISFSQPTCPDHMLLNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETCEKLGYQWKKSCMNQVTISTTDRRNNKLIFKVNLLEMDDKILVDFRLSKGDGLEFKRHFLKIKGKLIDIVSSQKVWLPAT*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11265
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Sequence difference compared with BC004202 CDS: a558g (silent)