cDNA Clone TSC2

• Plasmid: pCMV5-HA TSC2
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-TSC2
• Genbank: NM_000548.3
• Tag: HA
• Species: Human

Sequence of Insert

ggatccATGgccaaaccaacaagcaaagattcaggcttgaaggagaagtttaagattctgttgggactgggaacaccgaggccaaatcccaggtctgcagagggtaaacagacggagtttatcatcaccgcggaaatactgagagaactgagcatggaatgtggcctcaacaatcgcatccggatgatagggcagatttgtgaagtcgcaaaaaccaagaaatttgaagagcacgcagtggaagcactctggaaggcggtcgcggatctgttgcagccggagcggccgctggaggcccggcacgcggtgctggctctgctgaaggccatcgtgcaggggcagggcgagcgtttgggggtcctcagagccctcttctttaaggtcatcaaggattacccttccaacgaagaccttcacgaaaggctggaggttttcaaggccctcacagacaatgggagacacatcacctacttggaggaagagctggctgactttgtcctgcagtggatggatgttggcttgtcctcggaattccttctggtgctggtgaacttggtcaaattcaatagctgttacctcgacgagtacatcgcaaggatggttcagatgatctgtctgctgtgcgtccggaccgcgtcctctgtggacatagaggtctccctgcaggtgctggacgccgtggtctgctacaactgcctgccggctgagagcctcccgctgttcatcgttaccctctgtcgcaccatcaacgtcaaggagctctgcgagccttgctggaagctgatgcggaacctccttggcacccacctgggccacagcgccatctacaacatgtgccacctcatggaggacagagcctacatggaggacgcgcccctgctgagaggagccgtgttttttgtgggcatggctctctggggagcccaccggctctattctctcaggaactcgccgacatctgtgttgccatcattttaccaggccatggcatgtccgaacgaggtggtgtcctatgagatcgtcctgtccatcaccaggctcatcaagaagtataggaaggagctccaggtggtggcgtgggacattctgctgaacatcatcgaacggctccttcagcagctccagaccttggacagcccggagctcaggaccatcgtccatgacctgttgaccacggtggaggagctgtgtgaccagaacgagttccacgggtctcaggagagatactttgaactggtggagagatgtgcggaccagaggcctgagtcctccctcctgaacctgatctcctatagagcgcagtccatccacccggccaaggacggc
tggattcagaacctgcaggcgctgatggagagattcttcaggagcgagtc
ccgaggcgccgtgcgcatcaaggtgctggacgtgctgtcctttgtgctgc
tcatcaacaggcagttctatgaggaggagctgattaactcagtggtcatc
tcgcagctctcccacatccccgaggataaagaccaccaggtccgaaagct
ggccacccagttgctggtggacctggcagagggctgccacacacaccact
tcaacagcctgctggacatcatcgagaaggtgatggcccgctccctctcc
ccacccccggagctggaagaaagggatgtggccgcatactcggcctcctt
ggaggatgtgaagacagccgtcctggggcttctggtcatccttcagacca
agttgtacaccctgcctgcaagccacgccacgcgtgtgtatgagatgctg
gtcagccacattcagctccactacaagcacagctacaccctgccaatcgc
gagcagcatccggctgcaggcctttgacttcctgttgctgctgcgggccg
actcactgcaccgcctgggcctgcccaacaaggatggagtcgtgcggttc
agcccctactgcgtctgcgactacatggagccagagagaggctctgagaa
gaagaccagcggccccctttctcctcccacagggcctcctggcccggcgc
ctgcaggccccgccgtgcggctggggtccgtgccctactccctgctcttc
cgcgtcctgctgcagtgcttgaagcaggagtctgactggaaggtgctgaa
gctggttctgggcaggctgcctgagtccctgcgctataaagtgctcatct
ttacttccccttgcagtgtggaccagctgtgctctgctctctgctccatg
ctttcaggcccaaagacactggagcggctccgaggcgccccagaaggctt
ctccagaactgacttgcacctggccgtggttccagtgctgacagcattaa
tctcttaccataactacctggacaaaaccaaacagcgcgagatggtctac
tgcctggagcagggcctcatccaccgctgtgccagccagtgcgtcgtggc
cttgtccatctgcagcgtggagatgcctgacatcatcatcaaggcgctgc
ctgttctggtggtgaagctcacgcacatctcagccacagccagcatggcc
gtcccactgctggagttcctgtccactctggccaggctgccgcacctcta
caggaactttgccgcggagcagtatgccagtgtgttcgccatctccctgc
cgtacaccaacccctccaagtttaatcagtacatcgtgtgtctggcccat
cacgtcatagccatgtggttcatcaggtgccgcctgcccttccggaagga
ttttgtccctttcatcactaagggcctgcggtccaatgtcctcttgtctt
ttgatgacacccccgagaaggacagcttcagggcccggagtactagtctc
aacgagagacccaagagtctgaggatagccagaccccccaaacaaggctt
gaataactctccacccgtgaaagaattcaaggagagctctgcagccgagg
ccttccggtgccgcagcatcagtgtgtctgaacatgtggtccgcagcagg
atacagacgtccctcaccagtgccagcttggggtctgcagatgagaactc
cgtggcccaggctgacgatagcctgaaaaacctccacctggagctcacgg
aaacctgtctggacatgatggctcgatacgtcttctccaacttcacggct
gtcccgaagaggtctcctgtgggcgagttcctcctagcgggtggcaggac
caaaacctggctggttgggaacaagcttgtcactgtgacgacaagcgtgg
gaaccgggacccggtcgttactaggcctggactcgggggagctgcagtcc
ggcccggagtcgagctccagccccggggtgcatgtaagacagaccaagga
ggcgccggccaagctggagtcccaggctgggcagcaggtgtcccgtgggg
cccgggatcgggtccgttccatgtcggggggccatggtcttcgagttggc
gccctggacgtgccggcctcccagttcctgggcagtgccacttctccagg
accacggactgcaccagccgcgaaacctgagaaggcctcagctggcaccc
gggttcctgtgcaggagaagacgaacctggcggcctatgtgcccctgctg
acccagggctgggcggagatcctggtccggaggcccacagggaacaccag
ctggctgatgagcctggagaacccgctcagccctttctcctcggacatca
acaacatgcccctgcaggagctgtctaacgccctcatggcggctgagcgc
ttcaaggagcaccgggacacagccctgtacaagtcactgtcggtgccggc
agccagcacggccaaaccccctcctctgcctcgctccaacacagactccg
ccgtggtcatggaggagggaagtccgggcgaggttcctgtgctggtggag
cccccagggttggaggacgttgaggcagcgctaggcatggacaggcgcac
ggatgcctacagcaggtcgtcctcagtctccagccaggaggagaagtcgc
tccacgcggaggagctggttggcaggggcatccccatcgagcgagtcgtc
tcctcggagggtggccggccctctgtggacctctccttccagccctcgca
gcccctgagcaagtccagctcctctcccgagctgcagactctgcaggaca
tcctcggggaccctggggacaaggccgacgtgggccggctgagccctgag
gttaaggcccggtcacagtcagggaccctggacggggaaagtgctgcctg
gtcggcctcgggcgaagacagtcggggccagcccgagggtcccttgcctt
ccagctccccccgctcgcccagtggcctccggccccgaggttacaccatc
tccgactcggccccatcacgcaggggcaagagagtagagagggacgcctt
aaagagcagagccacagcctccaatgcagagaaagtgccaggcatcaacc
ccagtttcgtgttcctgcagctctaccattcccccttctttggcgacgag
tcaaacaagccaatcctgctgcccaatgagtcacagtcctttgagcggtc
ggtgcagctcctcgaccagatcccatcatacgacacccacaagatcgccg
tcctgtatgttggagaaggccagagcaacagcgagctcgccatcctgtcc
aatgagcatggctcctacaggtacacggagttcctgacgggcctgggccg
gctcatcgagctgaaggactgccagccggacaaggtgtacctgggaggcc
tggacgtgtgtggtgaggacggccagttcacctactgctggcacgatgac
atcatgcaagccgtcttccacatcgccaccctgatgcccaccaaggacgt
ggacaagcaccgctgcgacaagaagcgccacctgggcaacgactttgtgt
ccattgtctacaatgactccggtgaggacttcaagcttggcaccatcaag
ggccagttcaactttgtccacgtgatcgtcaccccgctggactacgagtg
caacctggtgtccctgcagtgcaggaaagacatggagggccttgtggaca
ccagcgtggccaagatcgtgtctgaccgcaacctgcccttcgtggcccgc
cagatggccctgcacgcaaatatggcctcacaggtgcatcatagccgctc
caaccccaccgatatctacccctccaagtggattgcccggctccgccaca
tcaagcggctccgccagcggatctgcgaggaagccgcctactccaacccc
agcctacctctggtgcaccctccgtcccatagcaaagcccctgcacagac
tccagccgagcccacacctggctatgaggtgggccagcggaagcgcctca
tctcctcggtggaggacttcaccgagtttgtgtgagtcgac

Amino Acid Sequence

MYPYDVPDYAGSMAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEF
IITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADL
LQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHE
RLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNS
CYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPL
FIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMED
APLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEI
VLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLL
TTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAK
DGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSV
VISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARS
LSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYE
MLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVV
RFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSL
LFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALC
SMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREM
VYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATAS
MAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCL
AHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARST
SLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVR
SRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNF
TAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGEL
QSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLR
VGALDVPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVP
LLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAA
ERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTDSAVVMEEGSPGEVPVL
VEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIER
VVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLS
PEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGY
TISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFG
DESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAI
LSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWH
DDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGT
IKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFV
ARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYS
NPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11440
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Derived from DU11409 (pRK7 FLAG TSC2) obtained from Blenis lab.; Sequence differences compared with NM_000548.3 CDS:; c1723t (L575sil), g3306a (V1102sil), delta t3815-g3883 (delta V1272-A1294 - also deleted in some published sequences e.g. AAC342101, EAW85559); ; Estimated MW of expressed protein : 199449 Daltons