cDNA Clone ALMS1

• Plasmid: pCMV5-HA ALMS1 G2401-V3851
• Parent Plasmid: pCMV5 HA3
• Expressed: HA ALMS1 G2401-V3851
• Genbank: AAH50330 (Has some residue differences compared with NM_015120.4)
• Tag: HA
• Species: Human

Sequence of Insert

gtcgacgggaataaaattattatccctatgatgactgtcataaaaagtgattcaagtagtgatgccagtgatggaaatggttcctgctcgtgggacagtaatttaccagagtctttggaatcagtttctgatgttcttctaaacttctttccatatgtttcacccaagacaagtataacagatagcagggaggaagagggtgtgtcagagagtgaggatggtggtggtagcagtgtagattcactggctgcacatgtgaaaaaccttctgcaatgtgaatcctcactgaatcatgctaaagaaata
ctcagaaatgcagaggaagaggaaagccgggtacgagcacatgcctggaa
tatgaagttcaatttagcacatgattgtggatactccatttcagaattaa
atgaagatgacaggaggaaagtagaagagatcaaggcagagttatttggt
catggaagaacaactgacttgtccaagggtttacagagtccacggggaat
gggatgcaagccagaagctgtatgtaAtcacattattattgagagccatg
aaaagggatgtttccggactctaacttctgaacatccacaactagataga
cacccttgtgctttcagatctgctggaccctcagaaatgaccagaggacg
gcagaacccatcatcatgcagagccaagcatgtcaacctttctgcatcct
tagaccagaacaactcccatttcaaagtttggaattccttgcagttaaaa
agtcattccccatttcagaactttatacctgatgaattcaaaattagcaa
aggtcttcgaatgccattcCatgaaaagatggacccttggctgtcagaat
tagtagaacctgcttttgtgccacctaaagaagtggattttcattcttca
tcacaaatgccgtccccagaacccatgaaaaagtttactacctccatcac
tttttcatctcaccgacattctaaatgcatttccaattcctctgttgtta
aggttggtgttactgaaggtagccagtgtactggagcatctgtgggggta
tttaattctcatttcactgaagaacaaaatcctcccagagatcttaaaca
gaaaacctcttccccttcatcatttaaaatgcatagtaattcacaagata
aagaagtgactattttagcagaaggtagaaggcaaagccaaaaattacct
gttgattttgagcgttcttttcaagaagaaaaacccttagaaagatcaga
ttttacaggcagtcattctgagcccagtaccagggcaaattgtagcaatt
tcaaggaaattcagatttctgataaccatacccttattagcatgggcaga
ccaagttccaccctaggagtaaacagatcgagttccagactaggagtaaa
agagaagaatgtaactataactccagatcttccttcttgcatttttcttg
aacaacgagagctctttgaacaaagcaaagccccacgtgcagatgaccat
gtgaggaaacaccattctccctctcctcaacatcaggattatgtagctcc
agaccttccttcttgcatttttcttgaacaacgagaactctttgaacagt
gcaaagccccatatgtagatcatcaaatgagagaaaaccattctcccctt
cctcaaggtcaggattctatagcttcagaccttccgtctcccatttctct
tgaacaatgccaaagcaaagcgccaggtgtagatgaccaaatgaataaac
accattttccccttcctcaaggtcaggattgtgtagtggaaaagaataat
caacataagcctaaatcacacatttctaatataaatgttgaagccaagtt
caatactgtggtctcccagtcagccccaaatcactgtacattagcagcat
ctgcatctactcctccttcaaatagaaaagcactttcttgtgttcatata
actctttgtcccaagacttcttccaagttggatagtggaactttagatga
aagattccattcattggatgctgcttctaaagcgaggatgaatagtgagt
ttaactttgacttacatactgtatcttcgagatcactggaaccaacctcc
aaattattgaccagtaaacctgtagcacaggatcaagaatctttaggttt
tctaggacctaaatcttcactggatttccaagtcgtacagccttctcttc
cagacagtaacactattactcaggacttgaaaaccataccttctcagaat
agccagatagtaacctccaggcaaatacaagtgaacatttcagatttcga
aggacattccaatccagaggggaccccagtatttgcagatcgattaccag
agaagatgaagaccccactttctgctttctctgaaaaattgtcatctgat
gcagtcactcagataacaacagaaagtccagaaaagaccctattttcatc
tgagatttttattaatgctgaagatcgtggacatgaaattatagagcctg
gtaaccagaagctacgcaaagctcctgtcaagtttgcctcatcatcttca
gtccaacaggttactttttctcgcggcacagatggccagcctttattatt
gccatataagccttctggtagtaccaagatgtattatgttccacaattaa
gacaaattcctccatctccggattccaaatcagataccaccgttgaaagc
tcccattcaggatccaatgatgccattgctccagacttcccagctcaggt
gctaggcacaagagatgatgacctctcagccactgttaacattaaacata
aagaaggaatctacagtaagagggtagtgactaaggcatccttgccagtg
ggagaaaaacccttgcagaatgaaaatgcagatgcctcagttcaagtgct
aatcactggggatgagaacctctcagacaaaaaacagcaagagattcaca
gtacaagggcagtgactgaggctgcccaggctaaagaaaaagaatctttg
cagaaagatactgcagattccagtgctgctgctgctgcagagcactcagc
tcTagtaggagacccagaaatgaagaacttgccagacactaaagccatta
cacagaaagaggagatccataggaagaagacagttcccgaggaagcctgg
ccaaacaataaagaatccctacagatcaatattgaagagtccgaatgtca
ttcagaatttgaaaatactacccgttctgtcttcaggtcagcaaagtttt
acattcatcatcccgtacacctaccaagtgatcaagatatttgccatgaa
tctttgggaaagagtgttttcatgagacattcttggaaagatttctttca
gcatcatccagacaaacatagagaacacatgtgtcttcctcttccttatc
aaaacatggacaagactaagacagattataccagaataaagagcctcagc
atcaatgtgaatttgggaaacaaagaagtgatggatactactaaaagtca
agttagagattatccaaaacataatggacaaattagtgatccacaaaggg
atcagaaggtcaccccagagcaaacaactcagcacactgtgagtttgaat
gaactgtggaacaagtatcgggagcgacagaggcaacagagacagcctga
gttgggtgacaggaaagaactgtccttggtggaccgacttgatcgtttgg
ctaaaattcttcagaatccaatcacacattctctccaggtctcagaaagt
acacatgatgatagcagaggggaacgaagtgtgaaggaatggagtggtag
acaacagcagagaaataagcttcagaaaaagaagcggtttaaaagcctag
agaaaagccataaaaatacaggcgagcttaaaaaaagcaaggtgctttct
catcatcgagctgggaggtctaatcaaattaaaattgaacagattaaatt
tgataaatatattctgagtaaacagccaggttttaattatataagcaaca
cttcttcggattgtcggccctcagaggagagtgagctgctcacagatact
accaccaacatcctttccggcaccacttctactgtcgaatcagatatatt
gacccaaacagatagagaggtggctctgcacgaaaggagtagctctgttt
ccactattgacactgcccggctgattcaagcttttggccatgaaagagta
tgcttgtcacccagacgaattaaattatatagcagcatcaccaaccaaca
gaggagataccttgagaagcggagcaaacacagcaagaaagtgctgaata
caggtcatcccctagtgacttctgagcacaccagaaggagacacatccag
gtataagcggccgc

Amino Acid Sequence

MYPYDVPDYAGSPEFPVDGNKIIIPMMTVIKSDSSSDASDGNGSCSWDSN
LPESLESVSDVLLNFFPYVSPKTSITDSREEEGVSESEDGGGSSVDSLAA
HVKNLLQCESSLNHAKEILRNAEEEESRVRAHAWNMKFNLAHDCGYSISE
LNEDDRRKVEEIKAELFGHGRTTDLSKGLQSPRGMGCKPEAVCNHIIIES
HEKGCFRTLTSEHPQLDRHPCAFRSAGPSEMTRGRQNPSSCRAKHVNLSA
SLDQNNSHFKVWNSLQLKSHSPFQNFIPDEFKISKGLRMPFHEKMDPWLS
ELVEPAFVPPKEVDFHSSSQMPSPEPMKKFTTSITFSSHRHSKCISNSSV
VKVGVTEGSQCTGASVGVFNSHFTEEQNPPRDLKQKTSSPSSFKMHSNSQ
DKEVTILAEGRRQSQKLPVDFERSFQEEKPLERSDFTGSHSEPSTRANCS
NFKEIQISDNHTLISMGRPSSTLGVNRSSSRLGVKEKNVTITPDLPSCIF
LEQRELFEQSKAPRADDHVRKHHSPSPQHQDYVAPDLPSCIFLEQRELFE
QCKAPYVDHQMRENHSPLPQGQDSIASDLPSPISLEQCQSKAPGVDDQMN
KHHFPLPQGQDCVVEKNNQHKPKSHISNINVEAKFNTVVSQSAPNHCTLA
ASASTPPSNRKALSCVHITLCPKTSSKLDSGTLDERFHSLDAASKARMNS
EFNFDLHTVSSRSLEPTSKLLTSKPVAQDQESLGFLGPKSSLDFQVVQPS
LPDSNTITQDLKTIPSQNSQIVTSRQIQVNISDFEGHSNPEGTPVFADRL
PEKMKTPLSAFSEKLSSDAVTQITTESPEKTLFSSEIFINAEDRGHEIIE
PGNQKLRKAPVKFASSSSVQQVTFSRGTDGQPLLLPYKPSGSTKMYYVPQ
LRQIPPSPDSKSDTTVESSHSGSNDAIAPDFPAQVLGTRDDDLSATVNIK
HKEGIYSKRVVTKASLPVGEKPLQNENADASVQVLITGDENLSDKKQQEI
HSTRAVTEAAQAKEKESLQKDTADSSAAAAAEHSALVGDPEMKNLPDTKA
ITQKEEIHRKKTVPEEAWPNNKESLQINIEESECHSEFENTTRSVFRSAK
FYIHHPVHLPSDQDICHESLGKSVFMRHSWKDFFQHHPDKHREHMCLPLP
YQNMDKTKTDYTRIKSLSINVNLGNKEVMDTTKSQVRDYPKHNGQISDPQ
RDQKVTPEQTTQHTVSLNELWNKYRERQRQQRQPELGDRKELSLVDRLDR
LAKILQNPITHSLQVSESTHDDSRGERSVKEWSGRQQQRNKLQKKKRFKS
LEKSHKNTGELKKSKVLSHHRAGRSNQIKIEQIKFDKYILSKQPGFNYIS
NTSSDCRPSEESELLTDTTTNILSGTTSTVESDILTQTDREVALHERSSS
VSTIDTARLIQAFGHERVCLSPRRIKLYSSITNQQRRYLEKRSKHSKKVL
NTGHPLVTSEHTRRRHIQV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11515
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 164802 Daltons