cDNA Clone USE1

• Plasmid: pFastBacHTb USE1
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-USE1
• Genbank: NM_023079.3 GI:150417998
• Tag: 6His
• Species: Human

Sequence of Insert

ggatccatggcggagagtccgactgaggaggcggcaacggcgggcgccggggcggcgggccccggggcgagcagcgttgctggtgttgttggcgttagcggcagcggcggcgggttcgggccgcctttcctgccggatgtgtgggcggcggcggcggcagcgggcggggccgggggcccggggagcggcctggctccgctgcccgggctcccgccctcagccgctgcccacggggccgcgctgcttagccactgggaccccacgctcagctccgactgggacggcgagcgcaccgcgccgcagtgt
ctactccggatcaagcgggatatcatgtccatttataaggagcctcctcc
aggaatgttcgttgtacctgatactgttgacatgactaagattcatgcat
tgatcacaggcccatttgacactccttatgaagggggtttcttcctgttc
gtgtttcggtgtccgcccgactatcccatccacccacctcgggtcaaact
gatgacaacgggcaataacacagtgaggtttaaccccaacttctaccgca
atgggaaagtctgcttgagtattctaggtacatggactggacctgcctgg
agcccagcccagagcatctcctcagtgctcatctctatccagtccctgat
gactgagaacccctatcacaatgagcccggctttgaacaggagagacatc
caggagacagcaaaaactataatgaatgtatccggcacgagaccatcaga
gttgcagtctgtgacatgatggaaggaaagtgtccctgtcctgaacccct
acgaggggtgatggagaagtcctttctggagtattacgacttctatgagg
tggcctgcaaagatcgcctgcaccttcaaggccaaactatgcaggaccct
tttggagagaagcggggccactttgactaccagtccctcttgatgcgcct
gggactgatacgtcagaaagtgctggagaggctccataatgagaatgcag
aaatggactctgatagcagttcatctgggacagagacagaccttcatggg
agcctgagggtttagcggccgc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMAESPTEEAATAGAGAAGPGAS
SVAGVVGVSGSGGGFGPPFLPDVWAAAAAAGGAGGPGSGLAPLPGLPPSA
AAHGAALLSHWDPTLSSDWDGERTAPQCLLRIKRDIMSIYKEPPPGMFVV
PDTVDMTKIHALITGPFDTPYEGGFFLFVFRCPPDYPIHPPRVKLMTTGN
NTVRFNPNFYRNGKVCLSILGTWTGPAWSPAQSISSVLISIQSLMTENPY
HNEPGFEQERHPGDSKNYNECIRHETIRVAVCDMMEGKCPCPEPLRGVME
KSFLEYYDFYEVACKDRLHLQGQTMQDPFGEKRGHFDYQSLLMRLGLIRQ
KVLERLHNENAEMDSDSSSSGTETDLHGSLRV*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU11586
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; Sequence difference compared with NM_023079.3 CDS: ; c846t (silent); ; Estimated MW of expressed protein : 41580 Daltons