cDNA Clone TTBK1

• Plasmid: pCMV-FLAG TTBK1 wt full
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG-TTBK1 wt
• Genbank: NP_115927.1
• Tag: FLAG

Sequence of Insert

ATGCAGTGCCTAGCGGCAGCTCTTAAGGACGAAACCAACATGAGTGGGGGAGGGGAGCAGGCCGACATCCTGCCGGCCAACTACGTGGTCAAAGATCGCTGGAAGGTGCTGAAAAAGATCGGGGGCGGGGGCTTCGGTGAGATCTATGAGGCCATGGACCTGCTGACCAGGGAGAACGTGGCTCTCAAGGTGGAGTCAGCCCAACAGCCTAAGCAGGTCCTCAAGATGGAGGTGGCTGTGCTCAAAAAGCTTCAAGGCAAGGACCACGTGTGCAGGTTCATTGGCTGTGGCCGTAACGAGAAGTTTAACTATGTGGTGATGCAGCTCCAGGGCCGGAACCTGGCTGACCTGCGCCGCAGCCAGCCAAGGGGCACTTTCACGTTGAGTACCACACTGCGCCTGGGCAAGCAGATCCTGGAGTCCATTGAAGCCATTCACTCCGTGGGCTTCCTGCACCGTGACATCAAGCCGTCCAACTTTGCCATGGGCCGGCTGCCCTCGACCTACAGGAAGTGCTACATGTTGGACTTTGGGCTAGCCAGGCAGTACACTAACACCACTGGGGACGTCCGGCCTCCTCGGAATGTGGCCGGGTTCCGGGGGACTGTCCGCTACGCCTCGGTCAATGCTCACAAGAACCGGGAGATGGGCCGCCATGATGACCTGTGGTCCCTCTTCTACATGCTGGTGGAGTTTGCTGTGGGCCAGCTGCCCTGGAGGAAGATCAAAGACAAGGAGCAGGTGGGGATGATCAAAGAGAAATACGAGCACCGGATGCTACTGAAGCACATGCCATCCGAGTTTCATCTCTTCCTGGACCACATTGCCAGCCTCGACTACTTCACCAAGCCCGATTACCAGTTGATCATGTCAGTGTTTGAGAACAGCATGAAGGAGCGGGGCATTGCCGAGAACGAGGCCTTTGACTGGGAGAAGGCAGGCACTGATGCCCTCCTGTCCACGAGCACCTCCACCCCACCCCAGCAGAACACGCGACAGACCGCAGCCATGTTTGGAGTGGTCAATGTGACACCAGTGCCAGGGGATCTGCTGAGGGAAAATACCGAAGACGTGCTCCAGGGGGAACATCTGTCCGACCAGGAAAACGCTCCACCAATCCTGCCTGGAAGACCATCTGAGGGCCTCGGACCTAGTCCACACCTGGTGCCACATCCTGGAGGACCAGAAGCAGAAGTGTGGGAGGAAACCGACGTGAACCGAAATAAGCTGAGGATCAACATTGGCAAAAGCCCTTGCGTGGAGGAAGAGCAGTCCAGGGGAATGGGTGTGCCAAGCTCCCCTGTCAGAGCTCCCCCTGATAGCCCAACCACACCCGTGCGCTCCCTGCGATACAGGAGAGTCAATTCTCCTGAAAGTGAGCGACTCTCTACAGCAGACGGAAGGGTGGAACTGCCAGAGCGGCGCAGCCGAATGGATCTCCCTGGCAGTCCATCACGACAGGCCTGTTCTAGTCAGCCTGCTCAGATGCTGTCCGTGGACACTGGTCACGCCGATAGGCAGGCTTCTGGCAGAATGGACGTGAGCGCCTCCGTCGAACAGGAGGCACTGTCAAACGCCTTCAGAAGCGTGCCACTCGCCGAAGAGGAAGACTTTGATAGCAAGGAGTGGGTGATCATTGACAAGGAAACCGAGCTGAAAGATTTCCCACCAGGAGCAGAGCCATCTACAAGTGGAACTACCGATGAGGAACCTGAGGAACTGCGGCCACTCCCAGAGGAAGGAGAGGAACGAAGGAGACTGGGTGCAGAGCCTACAGTGCGCCCACGAGGCAGGAGCATGCAGGCACTCGCCGAGGAAGACCTGCAGCACCTCCCTCCACAGCCACTCCCACCTCAGCTGTCCCAGGGAGATGGACGCTCAGAGACCAGCCAGCCACCCACACCCGGTTCACCTAGCCACTCCCCACTGCATTCAGGACCTCGGCCACGGCGCCGAGAGTCCGACCCAACCGGACCACAGCGACAGGTGTTCTCCGTCGCTCCTCCATTTGAGGTCAATGGACTGCCAAGGGCAGTCCCTCTCAGCCTGCCATACCAGGATTTCAAGAGAGACCTGTCCGATTATAGAGAGCGAGCACGACTGCTCAACCGGGTGAGGAGAGTCGGATTTTCTCATATGCTGCTCACAACTCCTCAGGTGCCACTGGCACCCGTCCAGCCTCAGGCCAATGGGAAAGAGGAGGAAGAAGAGGAAGAGGAAGACGAGGAAGAGGAAGAGGAAGATGAAGAGGAAGAGGAAGAGGAGGAGGAGGAGGAGGAAGAAGAAGAAGAGGAGGAGGAGGAAGAGGAGGAGGCCGCTGCAGCCGTGGCCCTCGGAGAGGTCCTGGGGCCTAGAAGCGGTTCAAGCTCCGAAGGCAGCGAGAGATCTACTGACAGGAGCCAGGAGGGAGCTCCAAGCACCCTGCTCGCAGACGATCAGAAGGAGTCCCGAGGCAGGGCCTCTATGGCTGACGGAGATCTGGAGCCTGAAGAGGGGTCTAAAACTCTCGTGCTGGTCAGTCCAGGCGACATGAAGAAATCACCCGTGACCGCAGAGCTCGCACCCGACCCTGATCTGGGAACTCTCGCTGCACTGACCCCACAGCACGAGAGGCCACAGCCAACAGGTAGTCAGCTGGACGTGAGCGAACCTGGCACTCTCTCTAGTGTCCTGAAGTCCGAGCCAAAACCACCTGGACCTGGTGCTGGACTGGGAGCAGGGACTGTGACCACAGGAGTCGGGGGTGTGGCTGTCACATCAAGCCCTTTCACTAAGGTGGAACGCACCTTTGTCCACATCGCCGAGAAAACCCATCTGAACGTGATGTCCTCTGGCGGACAGGCTCTCAGGAGCGAAGAGTTTTCCGCAGGAGGTGAACTCGGACTGGAGCTCGCTTCCGACGGAGGAGCAGTGGAAGAGGGAGCTAGAGCACCCCTGGAGAACGGGCTGGCCCTCTCTGGACTCAATGGGGCTGAAATTGAGGGCAGTGCCCTGTCAGGAGCTCCACGAGAAACACCTTCCGAGATGGCTACTAACTCTCTCCCAAATGGACCCGCCCTGGCTGATGGACCTGCCCCAGTGAGCCCACTGGAACCATCCCCTGAGAAGGTCGCAACCATCAGCCCTCGGCGCCATGCTATGCCAGGCTCTAGACCCCGGAGTCGCATTCCTGTGCTGCTCAGTGAAGAGGACACAGGGTCAGAACCATCTGGTAGTCTGTCAGCCAAGGAGCGGTGGTCTAAACGAGCAAGGCCTCAGCAGGATCTCGCCCGCCTGGTGATGGAGAAGAGGCAGGGAAGACTGCTCCTGAGGCTGGCTAGCGGGGCAAGTTCAAGCTCCTCTGAAGAGCAGCGAAGGGCTTCAGAGACACTGAGCGGTACTGGCTCCGAAGAGGACACACCTGCATCCGAGCCAGCCGCTGCACTGCCACGAAAATCTGGCCGAGCCGCTGCAACTAGAAGTAGAATCCCCCGGCCCATTGGACTGCGCATGCCTATGCCAGTGGCCGCTCAGCAGCCTGCTAGCAGATCCCACGGAGCAGCACCAGCACTGGACACTGCCATCACCTCTCGACTGCAGCTCCAGACCCCACCAGGCAGTGCCACAGCTGCAGATCTGAGGCCAAAGCAGCCTCCAGGACGAGGACTCGGTCCAGGAAGGGCACAGGCAGGAGCTAGACCCCCTGCACCTCGGTCCCCACGACTGCCAGCATCTACAAGTGCCGCTAGAAACGCATCAGCCAGCCCACGGTCCCAGTCTCTGAGTAGACGGGAGTCACCCAGCCCTTCCCATCAGGCACGACCAGGAGTGCCACCACCTCGAGGTGTCCCACCCGCTCGAGCACAGCCAGACGGAACCCCATCTCCAGGAGGTAGTAAGAAAGGTCCCAGGGGCAAGCTGCAGGCCCAGAGGGCAACTACTAAAGGTCGGGCAGGAGGTGCTGAAGGAAGGGCAGGGGCAAGGTGA

Amino Acid Sequence

MQCLAAALKDETNMSGGGEQADILPANYVVKDRWKVLKKIGGGGFGEIYEAMDLLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNEKFNYVVMQLQGRNLADLRRSQPRGTFTLSTTLRLGKQILESIEAIHSVGFLHRDIKPSNFAMGRLPSTYRKCYMLDFGLARQYTNTTGDVRPPRNVAGFRGTVRYASVNAHKNREMGRHDDLWSLFYMLVEFAVGQLPWRKIKDKEQVGMIKEKYEHRMLLKHMPSEFHLFLDHIASLDYFTKPDYQLIMSVFENSMKERGIAENEAFDWEKAGTDALLSTSTSTPPQQNTRQTAAMFGVVNVTPVPGDLLRENTEDVLQGEHLSDQENAPPILPGRPSEGLGPSPHLVPHPGGPEAEVWEETDVNRNKLRINIGKSPCVEEEQSRGMGVPSSPVRAPPDSPTTPVRSLRYRRVNSPESERLSTADGRVELPERRSRMDLPGSPSRQACSSQPAQMLSVDTGHADRQASGRMDVSASVEQEALSNAFRSVPLAEEEDFDSKEWVIIDKETELKDFPPGAEPSTSGTTDEEPEELRPLPEEGEERRRLGAEPTVRPRGRSMQALAEEDLQHLPPQPLPPQLSQGDGRSETSQPPTPGSPSHSPLHSGPRPRRRESDPTGPQRQVFSVAPPFEVNGLPRAVPLSLPYQDFKRDLSDYRERARLLNRVRRVGFSHMLLTTPQVPLAPVQPQANGKEEEEEEEEDEEEEEEDEEEEEEEEEEEEEEEEEEEEEEEAAAAVALGEVLGPRSGSSSEGSERSTDRSQEGAPSTLLADDQKESRGRASMADGDLEPEEGSKTLVLVSPGDMKKSPVTAELAPDPDLGTLAALTPQHERPQPTGSQLDVSEPGTLSSVLKSEPKPPGPGAGLGAGTVTTGVGGVAVTSSPFTKVERTFVHIAEKTHLNVMSSGGQALRSEEFSAGGELGLELASDGGAVEEGARAPLENGLALSGLNGAEIEGSALSGAPRETPSEMATNSLPNGPALADGPAPVSPLEPSPEKVATISPRRHAMPGSRPRSRIPVLLSEEDTGSEPSGSLSAKERWSKRARPQQDLARLVMEKRQGRLLLRLASGASSSSSEEQRRASETLSGTGSEEDTPASEPAAALPRKSGRAAATRSRIPRPIGLRMPMPVAAQQPASRSHGAAPALDTAITSRLQLQTPPGSATAADLRPKQPPGRGLGPGRAQAGARPPAPRSPRLPASTSAARNASASPRSQSLSRRESPSPSHQARPGVPPPRGVPPARAQPDGTPSPGGSKKGPRGKLQAQRATTKGRAGGAEGRAGAR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU34395
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: partly synthesised hence the difference to NM_032539.1