cDNA Clone WNK4

• Plasmid: pCMV5 WNK4-H1145Q1156/AA
• Parent Plasmid: pCMV5
• Expressed: FLAG-WNK4 H1145Q1156/AA
• Genbank: NM_032387.4
• Tag: FLAG
• Species: Human

Sequence of Insert

AGATCCGCCACCATGGACTACAAGGACGACGATGACAAGTTGGCATCCCC
GGCCACGGAGACCACCGTCCTCATGTCCCAGACTGAGGCCGACCTGGCCC
TGCGGCCCCCGCCTCCTCTTGGCACCGCGGGGCAGCCCCGCCTCGGGCCC
CCTCCTCGCCGAGCGCGCCGCTTCTCCGGGAAGGCTGAGCCCCGGCCGCG
CTCTTCTCGTCTCAGCCGCCGTAGCTCAGTCGACTTGGGGCTGCTGAGCT
CTTGGTCCCTGCCAGCCTCACCCGCTCCGGACCCCCCCGATCCTCCGGAC
TCCGCTGGTCCTGGCCCCGCGAGGAGCCCACCGCCTAGCTCCAAAGAACC
CCCCGAGGGCACGTGGACCGAGGGAGCCCCTGTGAAGGCTGCGGAAGACT
CCGCGCGTCCCGAGCTCCCGGACTCTGCAGTGGGCCCGGGGTCCAGGGAG
CCGCTAAGGGTCCCTGAAGCTGTGGCCCTAGAGCGGCGGCGGGAGCAGGA
AGAAAAGGAGGACATGGAGACCCAGGCTGTGGCAACGTCCCCCGATGGCC
GATACCTCAAGTTTGACATCGAGATTGGACGTGGCTCCTTCAAGACGGTG
TATCGAGGGCTAGACACCGACACCACAGTGGAGGTGGCCTGGTGTGAGCT
GCAGACTCGGAAACTGTCTAGAGCTGAGCGGCAGCGCTTCTCAGAGGAGG
TGGAGATGCTCAAGGGGCTGCAGCACCCCAACATCGTCCGCTTCTATGAT
TCGTGGAAGTCGGTGCTGAGGGGCCAGGTTTGCATCGTGCTGGTCACCGA
ACTCATGACCTCGGGCACGCTCAAGACGTACCTGAGGCGGTTCCGGGAGA
TGAAGCCGCGGGTCCTTCAGCGCTGGAGCCGCCAAATCCTGCGGGGACTT
CATTTCCTACACTCCCGGGTTCCTCCCATCCTGCACCGGGATCTCAAGTG
CGACAATGTCTTTATCACGGGACCTACTGGCTCTGTCAAAATCGGGGACC
TGGGCCTGGCCACGCTCAAGCGCGCCTCCTTTGCCAAGAGTGTCATCGGG
ACCCCGGAATTCATGGCCCCCGAGATGTACGAGGAAAAGTACGATGAGGC
CGTGGACGTGTACGCGTTCGGCATGTGCATGCTGGAGATGGCCACCTCTG
AGTACCCGTACTCCGAGTGCCAGAATGCCGCGCAAATCTACCGCAAGGTC
ACTTCGGGCAGAAAGCCGAACAGCTTCCACAAGGTGAAGATACCCGAGGT
GAAGGAGATCATTGAAGGCTGCATCCGCACGGATAAGAACGAGAGGTTCA
CCATCCAGGACCTCCTGGCCCACGCCTTCTTCCGCGAGGAGCGCGGTGTG
CACGTGGAACTAGCGGAGGAGGACGACGGCGAGAAGCCGGGCCTCAAGCT
CTGGCTGCGCATGGAGGACGCGCGGCGCGGGGGGCGCCCACGGGACAACC
AGGCCATCGAGTTCCTGTTCCAGCTGGGCCGGGACGCGGCCGAGGAGGTG
GCACAGGAGATGGTGGCTCTGGGCTTGGTCTGTGAAGCCGATTACCAGCC
AGTGGCCCGTGCAGTACGTGAACGGGTTGCTGCCATCCAGCGAAAGCGTG
AGAAGCTGCGTAAAGCAAGGGAATTGGAGGCACTCCCACCAGAGCCAGGA
CCTCCACCAGCAACTGTGCCCATGGCCCCCGGTCCCCCCAGTGTCTTCCC
CCCTGAGCCTGAGGAGCCAGAGGCAGACCAGCACCAGCCCTTCCTTTTCC
GCCACGCCAGCTACTCATCTACCACTTCGGATTGCGAGACTGATGGCTAC
CTCAGCTCCTCCGGCTTCCTGGATGCCTCAGACCCTGCCCTTCAGCCCCC
TGGGGGGGTGCCATCCAGCCTGGCTGAGTCCCATCTCTGCCTGCCCTCGG
CTTTTGCCCTATCCATTCCACGTTCTGGCCCTGGAAGTGACTTTTCCCCC
GGGGACAGCTATGCCTCAGATGCAGCTTCAGGCCTTAGCGATGTGGGAGA
AGGGATGGGACAAATGAGGAGACCCCCAGGGAGGAATCTCCGGCGCAGAC
CCCGATCCCGGCTGCGGGTCACTAGTGTCTCAGACCAGAATGACAGAGTG
GTTGAGTGCCAGCTACAGACCCATAACAGCAAGATGGTGACCTTCCGATT
TGATCTGGATGGGGACAGCCCGGAAGAGATTGCAGCTGCCATGGTATATA
ACGAGTTCATTCTGCCTTCGGAGCGAGATGGATTTCTCAGACGGATTCGG
GAGATTATCCAGCGAGTGGAGACCCTGTTGAAGAGAGACACTGGCCCCAT
GGAGGCTGCTGAAGACACCCTAAGCCCCCAGGAGGAGCCAGCACCATTAC
CTGCCCTGCCCGTCCCCCTCCCAGACCCATCCAATGAAGAGCTCCAGAGC
AGCACCTCCCTGGAGCACAGGAGCTGGACAGCCTTCTCCACCTCCTCATC
TTCTCCTGGAACTCCTTTGTCTCCTGGAAACCCATTTTCCCCTGGAACCC
CCATTTCCCCAGGTCCCATCTTCCCCATCACTTCTCCCCCATGTCATCCC
AGCCCCTCCCCATTCTCCCCCATTTCTTCCCAGGTCTCCTCAAATCCCTC
TCCACACCCCACCAGCTCTCCACTTCCATTCTCCTCCAGCACACCCGAGT
TTCCGGTCCCACTCTCTCAGTGTCCCTGGAGTTCTCTCCCCACGACTTCT
CCACCTACGTTCTCTCCCACTTGTTCTCAGGTCACTCTTAGTTCCCCTTT
CTTTCCTCCGTGCCCCTCCACTTCTTCCTTCCCCTCCACCACAGCAGCCC
CTCTCCTTTCTCTGGCTAGTGCCTTCTCACTGGCTGTGATGACTGTGGCC
CAGTCCCTGCTGTCCCCCTCACCTGGGCTCCTTTCCCAGTCTCCTCCAGC
CCCTCCTAGTCCCCTCCCTAGCCTGCCCCTTCCCCCTCCCGTTGCTCCTG
GTGGCCAGGAAAGCCCTTCACCCCACACAGCTGAGGTGGAGAGTGAGGCC
TCACCACCTCCTGCTCGGCCCCTCCCAGGGGAAGCCAGGCTGGCGCCCAT
CTCTGAAGAGGGAAAGCCGCAGCTTGTTGGGCGTTTCCAAGTGACTTCAT
CCAAGGAACCGGCTGAGCCTCTTCCCTTGCAGCCAACATCCCCCACTCTC
TCTGGTTCTCCAAAACCTTCAACCCCTCAGCTCACTTCAGAGAGCTCAGA
TACAGAGGACAGTGCTGGAGGCGGGCCAGAGACCAGGGAAGCTCTGGCTG
AGAGCGACCGTGCAGCTGAGGGTCTGGGGGCTGGAGTTGAGGAGGAAGGA
GATGATGGGAAGGAACCCCAAGTTGGGGGCAGCCCCCAACCCCTGAGCCA
TCCCAGCCCAGTGTGGATGAACTACTCCTACAGCAGCCTGTGTTTGAGCA
GCGAGGAGTCAGAAAGCAGTGGGGAAGATGAGGAGTTCTGGGCTGAGCTG
CAGAGTCTTCGGCAGAAGGCCTTGTCAGAGGTGGAAACACTACAGACACT
AGCGAAAAAAGAAATTGAAGATTTGTACAGCCGGCTGGGGAAGCAGCCCC
CACCGGGTATTGTGGCCCCAGCTGCTATGCTGTCCAGCCGCCAGCGCCGC
CTCTCCAAGGGCAGCTTCCCCACCTCCCGCCGCAACAGCCTACAGCGCTC
TGAGCCCCCAGGCCCTGGCATCATGCGAAGGAACTCTCTGAGTGGCAGCA
GCACCGGCTCCCAGGAGCAGCGGGCAAGCAAGGGGGTGACATTCGCCGGG
GATGTTGGCAGGATGTGAAGCGGCCGCGACTCTAGAGGATCC

Amino Acid Sequence

MDYKDDDDKLASPATETTVLMSQTEADLALRPPPPLGTAGQPRLGPPPRR
ARRFSGKAEPRPRSSRLSRRSSVDLGLLSSWSLPASPAPDPPDPPDSAGP
GPARSPPPSSKEPPEGTWTEGAPVKAAEDSARPELPDSAVGPGSREPLRV
PEAVALERRREQEEKEDMETQAVATSPDGRYLKFDIEIGRGSFKTVYRGL
DTDTTVEVAWCELQTRKLSRAERQRFSEEVEMLKGLQHPNIVRFYDSWKS
VLRGQVCIVLVTELMTSGTLKTYLRRFREMKPRVLQRWSRQILRGLHFLH
SRVPPILHRDLKCDNVFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEF
MAPEMYEEKYDEAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGR
KPNSFHKVKIPEVKEIIEGCIRTDKNERFTIQDLLAHAFFREERGVHVEL
AEEDDGEKPGLKLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQEM
VALGLVCEADYQPVARAVRERVAAIQRKREKLRKARELEALPPEPGPPPA
TVPMAPGPPSVFPPEPEEPEADQHQPFLFRHASYSSTTSDCETDGYLSSS
GFLDASDPALQPPGGVPSSLAESHLCLPSAFALSIPRSGPGSDFSPGDSY
ASDAASGLSDVGEGMGQMRRPPGRNLRRRPRSRLRVTSVSDQNDRVVECQ
LQTHNSKMVTFRFDLDGDSPEEIAAAMVYNEFILPSERDGFLRRIREIIQ
RVETLLKRDTGPMEAAEDTLSPQEEPAPLPALPVPLPDPSNEELQSSTSL
EHRSWTAFSTSSSSPGTPLSPGNPFSPGTPISPGPIFPITSPPCHPSPSP
FSPISSQVSSNPSPHPTSSPLPFSSSTPEFPVPLSQCPWSSLPTTSPPTF
SPTCSQVTLSSPFFPPCPSTSSFPSTTAAPLLSLASAFSLAVMTVAQSLL
SPSPGLLSQSPPAPPSPLPSLPLPPPVAPGGQESPSPHTAEVESEASPPP
ARPLPGEARLAPISEEGKPQLVGRFQVTSSKEPAEPLPLQPTSPTLSGSP
KPSTPQLTSESSDTEDSAGGGPETREALAESDRAAEGLGAGVEEEGDDGK
EPQVGGSPQPLSHPSPVWMNYSYSSLCLSSEESESSGEDEEFWAELQSLR
QKALSEVETLQTLAKKEIEDLYSRLGKQPPPGIVAPAAMLSSRQRRLSKG
SFPTSRRNSLQRSEPPGPGIMRRNSLSGSSTGSQEQRASKGVTFAGDVGR
M*

• Vector Type: Mammalian
• DU Number: DU2812
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All WNK plasmids MUST be grown at 30 degrees C or less to prevent recombination.