cDNA Clone WNK4

• Plasmid: GEM-JUEL-Kdead WNK4
• Parent Plasmid: PGem-He-Juel-
• Expressed: GEM-Jue-Kdead-WNK4 in oocyte vector
• Genbank: AF390018
• Species: Human

Sequence of Insert

GGATCCGCCACCATGGACTACAAGGACGACGATGACAAGTTGGCATCCCCGGCCACGGAGACCACCGTCCTCATGTCCCAGACTGAGGCCGACCTGGCCCTGCGGCCCCCGCCTCCTCTTGGCACCGCGGGGCAGCCCCGCCTCGGGCCCCCTCCTCGCCGAGCGCGCCGCTTCTCCGGGAAGGCTGAGCCCCGGCCGCGCTCTTCTCGTCTCAGCCGCCGTAGCTCAGTCGACTTGGGGCTGCTGAGCTCTTGGTCCCTGCCAGCCTCACCCGCTCCGGACCCCCCCGATCCTCCGGACTCCGCTGGTCCTGGCCCCGCGAGGAGCCCACCGCCTAGCTCCAAAGAACCCCCCGAGGGCACGTGGACCGAGGGAGCCCCTGTGAAGGCTGCGGAAGACTCCGCGCGTCCCGAGCTCCCGGACTCTGCAGTGGGCCCGGGGTCCAGGGAGCCGCTAAGGGTCCCTGAAGCTGTGGCCCTAGAGCGGCGGCGGGAGCAGGAAGAAAAGGAGGACATGGAGACCCAGGCTGTGGCAACGTCCCCCGATGGCCGATACCTCAAGTTTGACATCGAGATTGGACGTGGCTCCTTCGCGACGGTGTATCGAGGGCTAGACACCGACACCACAGTGGAGGTGGCCTGGTGTGAGCTGCAGACTCGGAAACTGTCTAGAGCTGAGCGGCAGCGCTTCTCAGAGGAGGTGGAGATGCTCAAGGGGCTGCAGCACCCCAACATCGTCCGCTTCTATGATTCGTGGAAGTCGGTGCTGAGGGGCCAGGTTTGCATCGTGCTGGTCACCGAACTCATGACCTCGGGCACGCTCAAGACGTACCTGAGGCGGTTCCGGGAGATGAAGCCGCGGGTCCTTCAGCGCTGGAGCCGCCAAATCCTGCGGGGACTTCATTTCCTACACTCCCGGGTTCCTCCCATCCTGCACCGGGATCTCAAGTGCGACAATGTCTTTATCACGGGACCTACTGGCTCTGTCAAAATCGGGGCCCTGGGCCTGGCCACGCTCAAGCGCGCCTCCTTTGCCAAGAGTGTCATCGGGACCCCGGAATTCATGGCCCCCGAGATGTACGAGGAAAAGTACGATGAGGCCGTGGACGTGTACGCGTTCGGCATGTGCATGCTGGAGATGGCCACCTCTGAGTACCCGTACTCCGAGTGCCAGAATGCCGCGCAAATCTACCGCAAGGTCACTTCGGGCAGAAAGCCGAACAGCTTCCACAAGGTGAAGATACCCGAGGTGAAGGAGATCATTGAAGGCTGCATCCGCACGGATAAGAACGAGAGGTTCACCATCCAGGACCTCCTGGCCCACGCCTTCTTCCGCGAGGAGCGCGGTGTGCACGTGGAACTAGCGGAGGAGGACGACGGCGAGAAGCCGGGCCTCAAGCTCTGGCTGCGCATGGAGGACGCGCGGCGCGGGGGGCGCCCACGGGACAACCAGGCCATCGAGTTCCTGTTCCAGCTGGGCCGGGACGCGGCCGAGGAGGTGGCACAGGAGATGGTGGCTCTGGGCTTGGTCTGTGAAGCCGATTACCAGCCAGTGGCCCGTGCAGTACGTGAACGGGTTGCTGCCATCCAGCGAAAGCGTGAGAAGCTGCGTAAAGCAAGGGAATTGGAGGCACTCCCACCAGAGCCAGGACCTCCACCAGCAACTGTGCCCATGGCCCCCGGTCCCCCCAGTGTCTTCCCCCCTGAGCCTGAGGAGCCAGAGGCAGACCAGCACCAGCCCTTCCTTTTCCGCCACGCCAGCTACTCATCTACCACTTCGGATTGCGAGACTGATGGCTACCTCAGCTCCTCCGGCTTCCTGGATGCCTCAGACCCTGCCCTTCAGCCCCCTGGGGGGGTGCCATCCAGCCTGGCTGAGTCCCATCTCTGCCTGCCCTCGGCTTTTGCCCTATCCATTCCACGTTCTGGCCCTGGAAGTGACTTTTCCCCCGGGGACAGCTATGCCTCAGATGCAGCTTCAGGCCTTAGCGATGTGGGAGAAGGGATGGGACAAATGAGGAGACCCCCAGGGAGGAATCTCCGGCGCAGACCCCGATCCCGGCTGCGGGTCACTAGTGTCTCAGACCAGAATGACAGAGTGGTTGAGTGCCAGCTACAGACCCATAACAGCAAGATGGTGACCTTCCGATTTGATCTGGATGGGGACAGCCCGGAAGAGATTGCAGCTGCCATGGTATATAACGAGTTCATTCTGCCTTCGGAGCGAGATGGATTTCTCAGACGGATTCGGGAGATTATCCAGCGAGTGGAGACCCTGTTGAAGAGAGACACTGGCCCCATGGAGGCTGCTGAAGACACCCTAAGCCCCCAGGAGGAGCCAGCACCATTACCTGCCCTGCCCGTCCCCCTCCCAGACCCATCCAATGAAGAGCTCCAGAGCAGCACCTCCCTGGAGCACAGGAGCTGGACAGCCTTCTCCACCTCCTCATCTTCTCCTGGAACTCCTTTGTCTCCTGGAAACCCATTTTCCCCTGGAACCCCCATTTCCCCAGGTCCCATCTTCCCCATCACTTCTCCCCCATGTCATCCCAGCCCCTCCCCATTCTCCCCCATTTCTTCCCAGGTCTCCTCAAATCCCTCTCCACACCCCACCAGCTCTCCACTTCCATTCTCCTCCAGCACACCCGAGTTTCCGGTCCCACTCTCTCAGTGTCCCTGGAGTTCTCTCCCCACGACTTCTCCACCTACGTTCTCTCCCACTTGTTCTCAGGTCACTCTTAGTTCCCCTTTCTTTCCTCCGTGCCCCTCCACTTCTTCCTTCCCCTCCACCACAGCAGCCCCTCTCCTTTCTCTGGCTAGTGCCTTCTCACTGGCTGTGATGACTGTGGCCCAGTCCCTGCTGTCCCCCTCACCTGGGCTCCTTTCCCAGTCTCCTCCAGCCCCTCCTAGTCCCCTCCCTAGCCTGCCCCTTCCCCCTCCCGTTGCTCCTGGTGGCCAGGAAAGCCCTTCACCCCACACAGCTGAGGTGGAGAGTGAGGCCTCACCACCTCCTGCTCGGCCCCTCCCAGGGGAAGCCAGGCTGGCGCCCATCTCTGAAGAGGGAAAGCCGCAGCTTGTTGGGCGTTTCCAAGTGACTTCATCCAAGGAACCGGCTGAGCCTCTTCCCTTGCAGCCAACATCCCCCACTCTCTCTGGTTCTCCAAAACCTTCAACCCCTCAGCTCACTTCAGAGAGCTCAGATACAGAGGACAGTGCTGGAGGCGGGCCAGAGACCAGGGAAGCTCTGGCTGAGAGCGACCGTGCAGCTGAGGGTCTGGGGGCTGGAGTTGAGGAGGAAGGAGATGATGGGAAGGAACCCCAAGTTGGGGGCAGCCCCCAACCCCTGAGCCATCCCAGCCCAGTGTGGATGAACTACTCCTACAGCAGCCTGTGTTTGAGCAGCGAGGAGTCAGAAAGCAGTGGGGAAGATGAGGAGTTCTGGGCTGAGCTGCAGAGTCTTCGGCAGAAGCACTTGTCAGAGGTGGAAACACTACAGACACTACAGAAAAAAGAAATTGAAGATTTGTACAGCCGGCTGGGGAAGCAGCCCCCACCGGGTATTGTGGCCCCAGCTGCTATGCTGTCCAGCCGCCAGCGCCGCCTCTCCAAGGGCAGCTTCCCCACCTCCCGCCGCAACAGCCTACAGCGCTCTGAGCCCCCAGGCCCTGGCATCATGCGAAGGAACTCTCTGAGTGGCAGCAGCACCGGCTCCCAGGAGCAGCGGGCAAGCAAGGGGGTGACATTCGCCGGGGATGTTGGCAGGATGTGAGCGGCCGCGACTCTAGA

Amino Acid Sequence

MDYKDDDDKLASPATETTVLMSQTEADLALRPPPPLGTAGQPRLGPPPRRARRFSGKAEPRPRSSRLSRRSSVDLGLLSSWSLPASPAPDPPDPPDSAGPGPARSPPPSSKEPPEGTWTEGAPVKAAEDSARPELPDSAVGPGSREPLRVPEAVALERRREQEEKEDMETQAVATSPDGRYLKFDIEIGRGSFATVYRGLDTDTTVEVAWCELQTRKLSRAERQRFSEEVEMLKGLQHPNIVRFYDSWKSVLRGQVCIVLVTELMTSGTLKTYLRRFREMKPRVLQRWSRQILRGLHFLHSRVPPILHRDLKCDNVFITGPTGSVKIGALGLATLKRASFAKSVIGTPEFMAPEMYEEKYDEAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGRKPNSFHKVKIPEVKEIIEGCIRTDKNERFTIQDLLAHAFFREERGVHVELAEEDDGEKPGLKLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQEMVALGLVCEADYQPVARAVRERVAAIQRKREKLRKARELEALPPEPGPPPATVPMAPGPPSVFPPEPEEPEADQHQPFLFRHASYSSTTSDCETDGYLSSSGFLDASDPALQPPGGVPSSLAESHLCLPSAFALSIPRSGPGSDFSPGDSYASDAASGLSDVGEGMGQMRRPPGRNLRRRPRSRLRVTSVSDQNDRVVECQLQTHNSKMVTFRFDLDGDSPEEIAAAMVYNEFILPSERDGFLRRIREIIQRVETLLKRDTGPMEAAEDTLSPQEEPAPLPALPVPLPDPSNEELQSSTSLEHRSWTAFSTSSSSPGTPLSPGNPFSPGTPISPGPIFPITSPPCHPSPSPFSPISSQVSSNPSPHPTSSPLPFSSSTPEFPVPLSQCPWSSLPTTSPPTFSPTCSQVTLSSPFFPPCPSTSSFPSTTAAPLLSLASAFSLAVMTVAQSLLSPSPGLLSQSPPAPPSPLPSLPLPPPVAPGGQESPSPHTAEVESEASPPPARPLPGEARLAPISEEGKPQLVGRFQVTSSKEPAEPLPLQPTSPTLSGSPKPSTPQLTSESSDTEDSAGGGPETREALAESDRAAEGLGAGVEEEGDDGKEPQVGGSPQPLSHPSPVWMNYSYSSLCLSSEESESSGEDEEFWAELQSLRQKHLSEVETLQTLQKKEIEDLYSRLGKQPPPGIVAPAAMLSSRQRRLSKGSFPTSRRNSLQRSEPPGPGIMRRNSLSGSSTGSQEQRASKGVTFAGDVGRM*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU6124
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: DOES NOT CUT INSERT (LINEARISE): Nhe1.; ; All WNK plasmids MUST be grown at 30 degrees C or less to prevent recombination.