cDNA Clone WNK1 (C.elegans)

• Plasmid: pGEX-6-WNK1 (C.elegans) 285-660
• Parent Plasmid: pGEX6P1
• Expressed: GST-WNK1 (C.elegans) 285-660
• Genbank: NM_182275
• Tag: GST
• Species: Caenorhabditis elegans

Sequence of Insert

GGATCCATGGTCAGTGAAGAGGAAAGAGCACGAAAAGAGCAAGAGAAGCGAGAAGAAGAAGAGAAAGCAGCACGGAGAATTGATGTTGAAGATGATTTTGATGCGCAAGAAAAACCCATTGATAAGAGTAAAAATGGTCGATTCTTAAAGTTCGACGAAGAACTTGGTCGCGGATCGTTCAAGACAGTGTTTCGAGGGTTGGACACTGAAACCGGAGTGGCCGTTGCGTGGTGTGAGCTTCAGGAGAGTAAACTGAATAAGACTGAAAGGCAACGTTTTCGAGAAGAAGCTGAAATGCTCAAGGATCTGCAACATCCGAACATCGTACGATTCTACGATTATTGGGAGAGTGCGGATTTGTGTGGAAAACGGAAATATATTGTGCTTGTAACCGAATTGATGACTTCAGGAACATTAAAAATGTATTTGAAGCGATTTAAACGGATAAACATAAAGGTATTGAAATCTTGGCGCAGACAAATTCTGAAAGGGCTTTCATTTCTTCACACACGTAATCCACCTGTAATTCATCGTGATCTCAAGTGTGATAATATCTTTATCACTGGTACAACTGGTAGTGTAAAGATTGGAGATCTTGGATTGGCAACTCTTAAAAATAAATCATTCGCGAAATCTGTCATTGGAACTCCAGAATTCATGGCACCGGAAATGTACGAAGAGATGTATGATGAGAGTGTAGATGTCTACGCGTTTGGAATGTGTCTTCTTGAAATGGTTACCGGAGAATATCCATATTCTGAGTGTATGAATCCAGCGACAATCTACAGAAAAGTGATATCAGGTGTCAAACCAGAATGTTTCTCAAGAATTCCTGCACAATATCCTGAAATACGTGAGATAATCGATCGGTGTATTCGTGTGAGACGAGAAGAGAGAAGTACCGTGAAGCAATTGCTCGTTGACGATTTCTTCACTCCAGAAGATCTCATCGGAATTCGTGTTGAAATTAAGAATCGGGATGCTGATCTCAATGATCTTAACGTGGAAATTCAAATGCAACTCCGAGTTTACGATGAGAAAAAGAGAAAACAATATCGGTTCAAGGAGAACGAAGGACTTCAATTTGCATTTGATATTGAAAATGACAGCCCAGATGAAGTTGTTCAATAGAAGGGCGAATTATGCGAGATATCCATCACACTGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMVSEEERARKEQEKREEEEKAARRIDVEDDFDAQEKPIDKSKNGRFLKFDEELGRGSFKTVFRGLDTETGVAVAWCELQESKLNKTERQRFREEAEMLKDLQHPNIVRFYDYWESADLCGKRKYIVLVTELMTSGTLKMYLKRFKRINIKVLKSWRRQILKGLSFLHTRNPPVIHRDLKCDNIFITGTTGSVKIGDLGLATLKNKSFAKSVIGTPEFMAPEMYEEMYDESVDVYAFGMCLLEMVTGEYPYSECMNPATIYRKVISGVKPECFSRIPAQYPEIREIIDRCIRVRREERSTVKQLLVDDFFTPEDLIGIRVEIKNRDADLNDLNVEIQMQLRVYDEKKRKQYRFKENEGLQFAFDIENDSPDEVVQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU6330
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: AMPLIFIED FROM T. GARTNER CDNA; ; COMPARED TO NCBI NM_182275 V437 IS MISSING AND C442 IS R IN OUR CLONE.; ; All WNK plasmids MUST be grown at 30 degrees C or less to prevent recombination.