cDNA Clone WNK1 (C.elegans)

• Plasmid: pGEX-WNK1 (C.elegans) 2KB 130-796
• Parent Plasmid: pGEX6P1
• Expressed: GST-WNK1 (C.elegans) 2KB 130-796
• Genbank: NM_182275
• Tag: GST
• Species: Caenorhabditis elegans

Sequence of Insert

GAATTCGCCCTTGGATCCTCGTCAGCTGCAACATCGACAGCTTCTGCCATAATCCCATTTACCTCAAATGTAGCTACAAATCATCCACATCTTAATCACCACGTGTCGAGAATACCACAGGCGATTGTAACAGGAGGGACAAATGGTTCATTACCTCCATTATTAATATCACCGACGTCAGCAGCAGCCGCAACGCCGTTGATTTCAGGAAAAGCGGGTCCAATGTCACCATCAACGGGAAGTCCAATTAATGTGGCTGCTACAGTATTACAGAATGCAGTTTCAAGTCCACAACATAGTGTTTTTGATAGATCTAGGTTAAATAAAATTCCACCAAACACATCATTAGCATCGTCTTCGTCTCCGAGTGATGCTGCTAACAATGATAAACCTATACAACAACGTCATAGTATTTTATCGAATGTTCGAACTCTCACTCAAGCAATGGTAAACGATGGACCACGAACGTTGACTGGAGATGATATGGACAAGATGGTCAGTGAAGAGGAAAGAGCACGAAAAGAGCAAGAGAAGCGAGAAGAAGAAGAGAAAGCAGCACGGAGAATTGATGTTGAAGATGATTTTGATGCGCAAGAAAAACCCATTGATAAGAGTAAAAATGGTCGATTCTTAAAGTTCGACGAAGAACTTGGTCGCGGATCGTTCAAGACAGTGTTTCGAGGGTTGGACACTGAAACCGGAGTGGCCGTTGCGTGGTGTGAGCTTCAGGAGAGTAAACTGAATAAGACTGAAAGGCAACGTTTTCGAGAAGAAGCTGAAATGCTCAAGGATCTGCAACATCCGAACATCGTACGATTCTACGATTATTGGGAGAGTGCGGATTTGTGTGGAAAACGGAAATATATTGTGCTTGTAACCGAATTGATGACTTCAGGAACATTAAAAATGTATTTGAAGCGATTTAAACGGATAAACATGAAGGTATTGAAATCTTGGTGCAGACAAATTCTGAAAGGGCTTTCATTTCTTCACACACGTAATCCACCTGTAATTCATCGTGATCTCAAGTGTGATAATATCTTTATCACTGGTACAACTGGTAGTGTAAAGATTGGAGATCTTGGATTGGCAACTCTTAAAAATAAATCATTCGCGAAATCTGTCATTGGAACTCCAGAATTCATGGCACCGGAAATGTACGAAGAGATGTATGATGAGAGTGTAGATGTCTACGCGTTTGGAATGTGTCTTCTTGAAATGGTTACCGGAGAATATCCATATTCTGAGTGTATGAATCCAGCGACAATCTACAGAAAAGTGATATCAGGTGTCAAACCAGAATGTTTCTCAAGAATTCCTGCACAATATCCTGAAATACGTGAGATAATCGATCGGTGTATTCGTGTGAGACGAGAAGAGAGAAGTACCGTGAAGCAATTGCTCGTTGACGATTTCTTCACTCCAGAAGATCTCATCGGAATTCGTGTTGAAATTAAGAATCGGGATGCTGATCTCAATGATCTTAACGTGGAAATTCAAATGCAACTCCGAGTTTACGATGAGAAAAAGAGAAAACAATATCGGTTCAAGGAGAACGAAGGACTTCAATTTGCATTTGATATTGAAAATGACAGCCCAGATGAAGTTGTTCAACAGATGATCGAGCAACAACATATTCCAGATGAGGATACTCGAATGATAACAAAGTTGATAAAAGACAAAGTGGATGCGTTCAGAAGAGATCGAGATCATCGTTTATTGGAGATTAAAAGAGCAAAAGAGGAGGAAGAAAGGATACGAGAAGAGGCTGAGATTAAAGAGGAATTGAGGTTGAGAGCTGAAGCTAAAGAAAAGGAAAAGGAGAGGTTGGAGAAAGAACGATTAGAGAAAAAAGCTGCAGCTGCGGCCGCAGCCAATCCGAATCCTACACCAATTCCACCAACTCCAGCAACACCACACTCTTCTGCCCAACAACAGCCTATTCCACCACCACTATCAACTCAAACTTCGGCTGAAATACAGCAGTCTGCTCAACAGCCATCTGTACCTGTAACTAAGGGCGAATTC

Amino Acid Sequence

EFALGSSSAATSTASAIIPFTSNVATNHPHLNHHVSRIPQAIVTGGTNGSLPPLLISPTSAAAATPLISGKAGPMSPSTGSPINVAATVLQNAVSSPQHSVFDRSRLNKIPPNTSLASSSSPSDAANNDKPIQQRHSILSNVRTLTQAMVNDGPRTLTGDDMDKMVSEEERARKEQEKREEEEKAARRIDVEDDFDAQEKPIDKSKNGRFLKFDEELGRGSFKTVFRGLDTETGVAVAWCELQESKLNKTERQRFREEAEMLKDLQHPNIVRFYDYWESADLCGKRKYIVLVTELMTSGTLKMYLKRFKRINMKVLKSWCRQILKGLSFLHTRNPPVIHRDLKCDNIFITGTTGSVKIGDLGLATLKNKSFAKSVIGTPEFMAPEMYEEMYDESVDVYAFGMCLLEMVTGEYPYSECMNPATIYRKVISGVKPECFSRIPAQYPEIREIIDRCIRVRREERSTVKQLLVDDFFTPEDLIGIRVEIKNRDADLNDLNVEIQMQLRVYDEKKRKQYRFKENEGLQFAFDIENDSPDEVVQQMIEQQHIPDEDTRMITKLIKDKVDAFRRDRDHRLLEIKRAKEEEERIREEAEIKEELRLRAEAKEKEKERLEKERLEKKAAAAAAANPNPTPIPPTPATPHSSAQQQPIPPPLSTQTSAEIQQSAQQPSVPVTKGEF

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU6371
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: AMPLIFIED FROM T. GARTNER CDNA; ; COMPARED TO NCBI NM_182275 V437 IS MISSING AND I224 IS V AND I436 IS M IN OUR CLONE – V/IS IS NOT CONSERVED, AND THE 224 POSITION IS M IN BOTH WNK1 AND WNK4 SO I ACCEPT IT AS CORRECT VERSION.; ; All WNK plasmids MUST be grown at 30 degrees C or less to prevent recombination.