cDNA Clone USP9X

• Plasmid: pCMV-FLAG USP9X M1-E795
• Parent Plasmid: pCMV5 FLAG
• Expressed: FLAG-USP9X M1-E795 plus extra residues
• Genbank: NM_001039591.2
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCCCGGAATTCGCCCTTATGACAGCCACGACTCGTGGCTCTCCGGTCGGAGGGAATGACAACCAGGGCCAGGCTCCTGATGGACAGTCTCAGCCCCCCCTCCAACAGAATCAGACTTCATCGCCTGATTCTTCCAATGAAAATTCCCCGGCAACTCCCCCAGATGAGCAAGGTCAAGGTGATGCCCCACCACAGCTTGAAGATGAGGAACCTGCATTTCCACATACTGACTTGGCCAAGTTGGATGACATGATCAACAGGCCTCGATGGGTGGTTCCAGTTTTGCCGAAAGGGGAATTAGAAGTGCTTTTAGAAGCTGCTATTGATCTTAGTAAAAAGGGCCTTGATGTTAAAAGTGAAGCATGTCAGCGATTTTTCCGTGATGGGCTAACAATATCATTCACTAAAATTCTTACAGATGAAGCAGTGAGTGGCTGGAAGTTTGAAATTCATAGGTGTATTATTAACAATACTCATCGTCTGGTGGAGCTATGTGTGGCCAAGTTGTCCCAAGACTGGTTTCCACTTTTAGAACTTCTTGCCATGGCCTTAAATCCTCATTGCAAATTCCATATCTACAATGGTACACGTCCATGTGAATCAGTTTCCTCAAGTGTTCAGTTGCCTGAAGATGAACTCTTTGCTCGTTCTCCAGATCCTCGATCACCAAAGGGTTGGCTAGTGGATCTTCTCAACAAATTTGGCACTTTAAATGGGTTCCAGATTTTGCATGATCGTTTTATTAATGGATCAGCATTAAACGTTCAAATAATTGCAGCCCTTATTAAACCATTTGGGCAATGCTATGAGTTTCTCACTCTTCATACAGTGAAAAAGTACTTTCTTCCAATAATAGAAATGGTTCCACAGTTTCTAGAAAACTTAACTGATGAAGAACTGAAAAAAGAAGCAAAGAATGAAGCCAAAAATGATGCTCTTTCAATGATTATTAAATCTTTGAAGAATTTAGCTTCAAGGGTTCCAGGACAAGAAGAAACTGTTAAAAACTTAGAAATATTTAGGTTAAAAATGATACTTAGATTATTGCAAATTTCTTCTTTCAATGGAAAGATGAATGCACTGAATGAAGTTAATAAGGTGATATCTAGTGTATCATACTATACTCATCGACATGGTAATCCTGAGGAGGAAGAGTGGCTCACAGCTGAACGAATGGCAGAATGGATACAGCAGAACAATATCTTATCCATAGTGTTGCGAGATAGTCTTCATCAGCCACAGTATGTAGAAAAGTTAGAGAAGATTCTTCGTTTTGTCATCAAAGAAAAAGCTCTGACCTTACAGGATCTTGATAATATCTGGGCAGCACAGGCAGGGAAACATGAAGCCATTGTGAAGAATGTACATGATCTCCTGGCAAAATTGGCATGGGATTTTTCTCCTGAACAACTTGATCATCTTTTTGATTGTTTTAAGGCCAGTTGGACAAATGCGAGTAAAAAGCAACGTGAAAAGCTACTTGAGCTGATACGTCGTCTTGCAGAAGATGATAAAGATGGTGTGATGGCACACAAAGTGTTGAACCTTCTGTGGAATCTGGCTCACAGTGATGATGTGCCTGTAGATATCATGGACCTGGCTCTCAGTGCCCACATAAAAATACTAGATTACAGTTGCTCCCAGGACCGTGATACACAAAAGATCCAATGGATAGATCGCTTTATAGAAGAACTTCGCACAAATGACAAATGGGTTATTCCCGCACTGAAACAAATTAGAGAAATTTGTAGTTTGTTTGGTGAAGCGCCTCAAAATTTGAGTCAAACTCAGCGAAGTCCCCATGTGTTTTATCGCCATGACTTAATCAATCAACTTCAACACAATCATGCCCTAGTTACTTTGGTAGCAGAAAACCTTGCAACTTACATGGAAAGCATGAGACTATATGCTAGAGACCATGAAGATTATGACCCACAAACTGTGAGGCTGGGAAGTAGATATAGTCATGTTCAAGAAGTTCAAGAACGGCTTAACTTCCTTAGATTTTTATTGAAGGATGGTCAGCTGTGGCTATGTGCTCCTCAGGCAAAACAAATATGGAAATGCTTAGCTGAGAATGCAGTTTACCTTTGTGATCGTGAAGCCTGTTTTAAGTGGTATTCCAAGTTGATGGGGGATGAACCAGACTTAGATCCTGATATTAATAAGGACTTCTTTGAAAGTAATGTGCTTCAGCTTGATCCTTCTCTGTTAACTGAAAATGGAATGAAGTGTTTTGAGCGATTCTTCAAAGCTGTGAATTGTCGAGAAGGAAAACTAGTAGCAAAAAGGAGAGCCTATATGATGGATGACTTGGAGTTAATAGGATTAGATTACCTTTGGAGGGTCGTGATTCAGAGTAATGATGATATTGCCAGCAGAGCTATAGATCTCCTCAAAGAAGgGGCGAATTCCCGGGTCGACTCGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSPEFALMTATTRGSPVGGNDNQGQAPDGQSQPPLQQNQTSSPDSSNENSPATPPDEQGQGDAPPQLEDEEPAFPHTDLAKLDDMINRPRW
VVPVLPKGELEVLLEAAIDLSKKGLDVKSEACQRFFRDGLTISFTKILTDEAVSGWKFEIHRCIINNTHRLVELCVAKLSQDWFPLLELLAMALNPHCKF
HIYNGTRPCESVSSSVQLPEDELFARSPDPRSPKGWLVDLLNKFGTLNGFQILHDRFINGSALNVQIIAALIKPFGQCYEFLTLHTVKKYFLPIIEMVPQ
FLENLTDEELKKEAKNEAKNDALSMIIKSLKNLASRVPGQEETVKNLEIFRLKMILRLLQISSFNGKMNALNEVNKVISSVSYYTHRHGNPEEEEWLTAE
RMAEWIQQNNILSIVLRDSLHQPQYVEKLEKILRFVIKEKALTLQDLDNIWAAQAGKHEAIVKNVHDLLAKLAWDFSPEQLDHLFDCFKASWTNASKKQR
EKLLELIRRLAEDDKDGVMAHKVLNLLWNLAHSDDVPVDIMDLALSAHIKILDYSCSQDRDTQKIQWIDRFIEELRTNDKWVIPALKQIREICSLFGEAP
QNLSQTQRSPHVFYRHDLINQLQHNHALVTLVAENLATYMESMRLYARDHEDYDPQTVRLGSRYSHVQEVQERLNFLRFLLKDGQLWLCAPQAKQIWKCL
AENAVYLCDREACFKWYSKLMGDEPDLDPDINKDFFESNVLQLDPSLLTENGMKCFERFFKAVNCREGKLVAKRRAYMMDDLELIGLDYLWRVVIQSNDD
IASRAIDLLKEGANSRVDSSGRDPGWHPCDPSPVPLLALEVATPVPTSLVLIKLSCIILSD*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCMV-FLAG USP9X M1-E795606.86 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU6815
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: To obtain clean a PCR run, no extra nt was added to the primers (restriction site or STOP codon).; Suitable for combination to create full USP cDNA.; ; Changes compared to NM_021906:; Compared to NCBI seq. Insert between AA 147-148:IINNTHR; ; As the above insert was present in every PCR product, and also from HELA RNA, I consider it the correct choice.; ; All USP9X plasmids MUST be grown at 30 degrees C or less to prevent recombination.
• Low Temp: No