cDNA Clone WNK1

• Plasmid: pGEX-6-FLAG WNK1-2-661 S382/A
• Parent Plasmid: pGEX6P1
• Expressed: GST-WNK1-2-661 S382/A
• Tag: GST

Sequence of Insert

GGATCCACTAGTGGTACCATCGATATTGCCACCATGGACTACAAGGACGA
CGATGACAAgtctggcggcgccgcagagaagcagagcagcactcccggtt
ccctgttcctctcgccgccggctcctgcccccaagaatggctccagctcc
gattcctccgtgggggagaaactgggagccgcggccgccgacgctgtgac
cggcaggaccgaggagtacaggcgccgccgccacactatggacaaggaca
gccgtggggcggccgcgaccactaccaccactgagcaccgcttcttccgc
cggagcgtcatctgtgactccaatgccactgcactggagcttcccggcct
tcctctttccctgccccagcccagcatccccgcggctgtcccgcagagtg
ctccaccggagccccaccgggaagagaccgtgaccgccaccgccacttcc
caggtagcccagcagcctccagccgctgccgcccctggggaacaggccgt
cgcgggccctgccccctcgactgtccccagcagtaccagcaaagaccgcc
cagtgtcccagcctagccttgtggggagcaaagaggagccgccgccggcg
agaagtggcagcggcggcggcagcgccaaggagccacaggaggaacggag
ccagcagcaggatgatatcgaagagctggagaccaaggccgtgggaatgt
ctaacgatggccgctttctcaagtttgacatcgaaatcggcagaggctcc
tttaagacggtctacaaaggtctggacactgaaaccaccgtggaagtcgc
ctggtgtgaactgcaggatcgaaaattaacaaagtctgagaggcagagat
ttaaagaagaagctgaaatgttaaaaggtcttcagcatcccaatattgtt
agattttatgattcctgggaatccacagtaaaaggaaagaagtgcattgt
tttggtgactgaacttatgacgtctggaacacttaaaacgtatctgaaaa
ggtttaaagtgatgaagatcaaagttctaagaagctggtgccgtcagatc
cttaaaggtcttcagtttcttcatactcgaactccacctatcattcaccg
cgatcttaaatgtgacaacatctttatcaccggccctactggctcagtca
agattggagacctcggtctggcaaccctgaagcgggcttcttttgccaag
GCtgtgataggtaccccagagttcatggcccctgagatgtatgaggagaa
atatgatgaatccgttgacgtttatgcttttgggatgtgcatgcttgaga
tggctacatctgaatatccttactcggagtgccaaaatgctgcacagatc
taccgtcgcgtgaccagtggggtgaagccagccagttttgacaaagtagc
aattcctgaagtgaaggaaattattgaaggatgcatacgacaaaacaaag
atgaaagatattccatcaaagaccttttgaaccatgccttcttccaagag
gaaacaggagtacgggtagaattagcagaagaagatgatggagaaaaaat
agccataaaattatggctacgtattgaagatattaagaaattaaagggaa
aatacaaagataatgaagctattgagttttcttttgatttagagagagat
gtcccagaagatgttgcacaagaaatggtagagtctgggtatgtctgtga
aggtgatcacaagaccatggctaaagctatcaaagacagagtatcattaa
ttaagaggaaacgagagcagcggcagttggtacgggaggagcaagaaaaa
aaaaagcaggaagagagcagtctcaaacagcaggtagaacaatccagtgc
ttcccagacaggaatcaagcagctcccttctgctagcaccggcataccta
ctgcttctaccacttcagcttcagtttctacacaagtagaacctgaagaa
cctgaggcagatcaacatcaccaactacagtaccagcaacccagtatatc
tgtgttatctgatgggacggttgacagtggtcagggatccccggaattcC
CGGGTCGACTCGAGCGGCCGCATCGTGA

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSGSTSGTIDIATMDYKDDDD
KSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGR
TEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPL
SLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAG
PAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQ
QDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWC
ELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLV
TELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDL
KCDNIFITGPTGSVKIGDLGLATLKRASFAKAVIGTPEFMAPEMYEEKYD
ESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIP
EVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAI
KLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGD
HKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQ
TGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHHQLQYQQPSISVL
SDGTVDSGQGSPEFPGRLERPHR

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU10048
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Has N terminal FLAG after the Precission, Q638H cahnge cf wt and extra seq at C terminus (PEFPGRLERPHR); ; All WNK plasmids MUST be grown at 30 degrees C or less to prevent recombination.