cDNA Clone USP9X

• Plasmid: GFP-TAP USP9X N-Term 1-800
• Parent Plasmid: pEGFP TAP
• Expressed: GFP-TAP-USP9X N-Term 1-800
• Genbank: NM_021906, (NM_004652)
• Tag: GFP
• Tag: TAP
• Species: Human

Sequence of Insert

GGATCCCCGGAATTCGCCCTTATGACAGCCACGACTCGTGGCTCTCCGGTCGGAGGGAATGACAACCAGGGCCAGGCTCCTGATGGACAGTCTCAGCCCCCCCTCCAACAGAATCAGACTTCATCGCCTGATTCTTCCAATGAAAATTCCCCGGCAACTCCCCCAGATGAGCAAGGTCAAGGTGATGCCCCACCACAGCTTGAAGATGAGGAACCTGCATTTCCACATACTGACTTGGCCAAGTTGGATGACATGATCAACAGGCCTCGATGGGTGGTTCCAGTTTTGCCGAAAGGGGAATTAGAAGTGCTTTTAGAAGCTGCTATTGATCTTAGTAAAAAGGGCCTTGATGTTAAAAGTGAAGCATGTCAGCGATTTTTCCGTGATGGGCTAACAATATCATTCACTAAAATTCTTACAGATGAAGCAGTGAGTGGCTGGAAGTTTGAAATTCATAGGTGTATTATTAACAATACTCATCGTCTGGTGGAGCTATGTGTGGCCAAGTTGTCCCAAGACTGGTTTCCACTTTTAGAACTTCTTGCCATGGCCTTAAATCCTCATTGCAAATTCCATATCTACAATGGTACACGTCCATGTGAATCAGTTTCCTCAAGTGTTCAGTTGCCTGAAGATGAACTCTTTGCTCGTTCTCCAGATCCTCGATCACCAAAGGGTTGGCTAGTGGATCTTCTCAACAAATTTGGCACTTTAAATGGGTTCCAGATTTTGCATGATCGTTTTATTAATGGATCAGCATTAAACGTTCAAATAATTGCAGCCCTTATTAAACCATTTGGGCAATGCTATGAGTTTCTCACTCTTCATACAGTGAAAAAGTACTTTCTTCCAATAATAGAAATGGTTCCACAGTTTCTAGAAAACTTAACTGATGAAGAACTGAAAAAAGAAGCAAAGAATGAAGCCAAAAATGATGCTCTTTCAATGATTATTAAATCTTTGAAGAATTTAGCTTCAAGGGTTCCAGGACAAGAAGAAACTGTTAAAAACTTAGAAATATTTAGGTTAAAAATGATACTTAGATTATTGCAAATTTCTTCTTTCAATGGAAAGATGAATGCACTGAATGAAGTTAATAAGGTGATATCTAGTGTATCATACTATACTCATCGACATGGTAATCCTGAGGAGGAAGAGTGGCTCACAGCTGAACGAATGGCAGAATGGATACAGCAGAACAATATCTTATCCATAGTGTTGCGAGATAGTCTTCATCAGCCACAGTATGTAGAAAAGTTAGAGAAGATTCTTCGTTTTGTCATCAAAGAAAAAGCTCTGACCTTACAGGATCTTGATAATATCTGGGCAGCACAGGCAGGGAAACATGAAGCCATTGTGAAGAATGTACATGATCTCCTGGCAAAATTGGCATGGGATTTTTCTCCTGAACAACTTGATCATCTTTTTGATTGTTTTAAGGCCAGTTGGACAAATGCGAGTAAAAAGCAACGTGAAAAGCTACTTGAGCTGATACGTCGTCTTGCAGAAGATGATAAAGATGGTGTGATGGCACACAAAGTGTTGAACCTTCTGTGGAATCTGGCTCACAGTGATGATGTGCCTGTAGATATCATGGACCTGGCTCTCAGTGCCCACATAAAAATACTAGATTACAGTTGCTCCCAGGACCGTGATACACAAAAGATCCAATGGATAGATCGCTTTATAGAAGAACTTCGCACAAATGACAAATGGGTTATTCCCGCACTGAAACAAATTAGAGAAATTTGTAGTTTGTTTGGTGAAGCGCCTCAAAATTTGAGTCAAACTCAGCGAAGTCCCCATGTGTTTTATCGCCATGACTTAATCAATCAACTTCAACACAATCATGCCCTAGTTACTTTGGTAGCAGAAAACCTTGCAACTTACATGGAAAGCATGAGACTATATGCTAGAGACCATGAAGATTATGACCCACAAACTGTGAGGCTGGGAAGTAGATATAGTCATGTTCAAGAAGTTCAAGAACGGCTTAACTTCCTTAGATTTTTATTGAAGGATGGTCAGCTGTGGCTATGTGCTCCTCAGGCAAAACAAATATGGAAATGCTTAGCTGAGAATGCAGTTTACCTTTGTGATCGTGAAGCCTGTTTTAAGTGGTATTCCAAGTTGATGGGGGATGAACCAGACTTAGATCCTGATATTAATAAGGACTTCTTTGAAAGTAATGTGCTTCAGCTTGATCCTTCTCTGTTAACTGAAAATGGAATGAAGTGTTTTGAGCGATTCTTCAAAGCTGTGAATTGTCGAGAAGGAAAACTAGTAGCAAAAAGGAGAGCCTATATGATGGATGACTTGGAGTTAATAGGATTAGATTACCTTTGGAGGGTCGTGATTCAGAGTAATGATGATATTGCCAGCAGAGCTATAGATCTCCTCAAAGAAGGGCGAATTCCCGGGTCGACTCGAGCGGCCGCAAGGGCGAATTCTGCAGTCGACGGTACCGCGGGCCCGGTATCGACCGGATCTAGATAACTGATCATAATCAGCCATACCACA

Amino Acid Sequence

GSPEFALMTATTRGSPVGGNDNQGQAPDGQSQPPLQQNQTSSPDSSNENSPATPPDEQGQGDAPPQLEDEEPAFPHTDLAKLDDMINRPRWVVPVLPKGELEVLLEAAIDLSKKGLDVKSEACQRFFRDGLTISFTKILTDEAVSGWKFEIHRCIINNTHRLVELCVAKLSQDWFPLLELLAMALNPHCKFHIYNGTRPCESVSSSVQLPEDELFARSPDPRSPKGWLVDLLNKFGTLNGFQILHDRFINGSALNVQIIAALIKPFGQCYEFLTLHTVKKYFLPIIEMVPQFLENLTDEELKKEAKNEAKNDALSMIIKSLKNLASRVPGQEETVKNLEIFRLKMILRLLQISSFNGKMNALNEVNKVISSVSYYTHRHGNPEEEEWLTAERMAEWIQQNNILSIVLRDSLHQPQYVEKLEKILRFVIKEKALTLQDLDNIWAAQAGKHEAIVKNVHDLLAKLAWDFSPEQLDHLFDCFKASWTNASKKQREKLLELIRRLAEDDKDGVMAHKVLNLLWNLAHSDDVPVDIMDLALSAHIKILDYSCSQDRDTQKIQWIDRFIEELRTNDKWVIPALKQIREICSLFGEAPQNLSQTQRSPHVFYRHDLINQLQHNHALVTLVAENLATYMESMRLYARDHEDYDPQTVRLGSRYSHVQEVQERLNFLRFLLKDGQLWLCAPQAKQIWKCLAENAVYLCDREACFKWYSKLMGDEPDLDPDINKDFFESNVLQLDPSLLTENGMKCFERFFKAVNCREGKLVAKRRAYMMDDLELIGLDYLWRVVIQSNDDIASRAIDLLKEGRIPGSTRAAARANSAVDGTAGPVSTGSR

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU10132
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All USP9X plasmids MUST be grown at 30 degrees C or less to prevent recombination.